Biomedical Microdevices

, Volume 13, Issue 3, pp 533–538 | Cite as

Development and characterization of a disposable plastic microarray printhead

  • Matthias Grießner
  • Dave Hartig
  • Alexander Christmann
  • Carsten Pohl
  • Michaela Schellhase
  • Eva Ehrentreich-Förster
Article

Abstract

During the last decade microarrays have become a powerful analytical tool. Commonly microarrays are produced in a non-contact manner using silicone printheads. However, silicone printheads are expensive and not able to be used as a disposable. Here, we show the development and functional characterization of 8-channel plastic microarray printheads that overcome both disadvantages of their conventional silicone counterparts. A combination of injection-molding and laser processing allows us to produce a high quantity of cheap, customizable and disposable microarray printheads. The use of plastics (e.g., polystyrene) minimizes the need for surface modifications required previously for proper printing results. Time-consuming regeneration processes, cleaning procedures and contaminations caused by residual samples are avoided. The utilization of plastic printheads for viscous liquids, such as cell suspensions or whole blood, is possible. Furthermore, functional parts within the plastic printhead (e.g., particle filters) can be included. Our printhead is compatible with commercially available TopSpot devices but provides additional economic and technical benefits as compared to conventional TopSpot printheads, while fulfilling all requirements demanded on the latter. All in all, this work describes how the field of traditional microarray spotting can be extended significantly by low cost plastic printheads.

Keywords

Microarray Printhead Disposable Plastics Injection-molding 

Supplementary material

ESM 1

(MPG 19092 kb)

ESM 2

(MPG 9220 kb)

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Matthias Grießner
    • 1
    • 2
  • Dave Hartig
    • 1
  • Alexander Christmann
    • 1
  • Carsten Pohl
    • 1
  • Michaela Schellhase
    • 1
  • Eva Ehrentreich-Förster
    • 1
  1. 1.Fraunhofer Institute for Biomedical Engineering (IBMT)PotsdamGermany
  2. 2.Institute of Biochemistry and Biology, iPOC Research GroupUniversity of PotsdamGolmGermany

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