An on-chip whole blood/plasma separator with bead-packed microchannel on COC polymer
- 989 Downloads
A disposable on-chip whole blood/plasma separator, which is able to separate plasma from whole human blood by capillary force through a bead-packed microchannel, has been designed, fabricated and characterized in this work. Various sizes of silica beads were slurry-packed through a microchannel using a bump structure which held beads in a defined region. The bead-packed microchannel induces a capillary force which allows plasma to move forward through the bead-packed column more rapidly than red blood cells (RBCs). The blood/plasma separator with bead-packed microchannel has successfully separated plasma from the whole blood without haemolysis of RBCs. The separation method developed in this work can be applied to various on-chip stationary filtrations of RBC for point-of-care clinical diagnostics.
KeywordsBead-packed microchannel Blood/plasma separator On-chip filtration Lab on a chip Slurry-packed microcolumn
The authors would like to acknowledge Mr. Matthew Sperling in University of Cincinnati Department of Emergency Medicine for procuring human blood samples.
- C.H. Ahn, J. Choi, G. Beaucage, J.H. Nevin, J. Lee, A. Puntambeker, J.Y. Lee, Proceedings of the IEEE, Special Issue on Biomedical Applications for MEMS and Microfluidics, 92, 154–173 (2004).Google Scholar
- J. Han, S. Lee, Y. Heo, C. Hwang, C.H. Ahn, Proceedings of 13th International Conference on Solid-State Sensors, Actuators and Microsystems (Transducers’05), 1688 (2005).Google Scholar
- E. Oosterbroek, A.v.d. Berg. Lab-on-a-Chip: Miniaturized Systems for (Bio)Chemical Analysis and Synthesis, 2nd edn (Elsevier Science, 2003).Google Scholar