Biomedical Microdevices

, Volume 12, Issue 3, pp 555–568 | Cite as

Hydrogel cell patterning incorporating photocaged RGDS peptides

  • Catherine A. Goubko
  • Swapan Majumdar
  • Ajoy Basak
  • Xudong Cao
Article

Abstract

As we aim towards enhancing our knowledge of complex cell behaviors and developing intricate cell-based devices and improved therapeutics, it becomes imperative that we be able to control and manipulate the spatial localization of cells. Here we have developed a novel strategy to pattern cells using a hyaluronic acid hydrogel material and photocaged RGDS (Arg-Gly-Asp-Ser) peptides. In this report, we discuss the chemical synthesis and photoactive properties of the caged peptides as well as the subsequent binding of these peptides to our hydrogel base. We further demonstrate the ability of this modified hydrogel material to pattern fibroblast cells on the micron scale using near-UV light exposure through a patterned photomask to selectively switch areas of the hydrogel surface from cell non-adhesive to cell adhesive. The cells are found to adhere and proliferate along the developed line patterns for at least 2.5 days, demonstrating significantly enhanced pattern longevity in comparison with previously reported studies.

Keywords

Cell adhesion Extracellular matrix Micropatterning Hyaluronic acid Peptide 

Supplementary material

10544_2010_9412_MOESM1_ESM.doc (10.4 mb)
Supplemental Fig. 1S1H NMR spectrum of caged-R[G]DS tetrapeptide in D2O with expanded portions within the insets (DOC 10628 kb)
10544_2010_9412_MOESM2_ESM.doc (10.4 mb)
Supplemental Fig. 2S1H NMR spectrum of caged R[G]DS tetrapeptide in D2O following UV-irradiation with expanded portions within the insets (DOC 10663 kb)

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Catherine A. Goubko
    • 1
  • Swapan Majumdar
    • 2
  • Ajoy Basak
    • 2
  • Xudong Cao
    • 1
    • 3
  1. 1.Department of Chemical and Biological EngineeringUniversity of OttawaOttawaCanada
  2. 2.Ottawa Health Research Institute, Loeb Building, Chronic Diseases ProgramThe Ottawa HospitalOttawaCanada
  3. 3.Ottawa-Carleton Institute for Biomedical EngineeringUniversity of OttawaOttawaCanada

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