Biomedical Microdevices

, Volume 12, Issue 1, pp 63–70 | Cite as

Nanostructured conducting polymer based reagentless capacitive immunosensor

  • Amay Jairaj Bandodkar
  • Chetna Dhand
  • Sunil K. Arya
  • M. K. Pandey
  • Bansi D. Malhotra


Nanostructured polyaniline (PANI) film electrophoretically fabricated onto indium-tin-oxide (ITO) coated glass plate has been utilized for development of an immunosensor based on capacitance change of a parallel plate capacitor (PPC) by covalently immobilizing anti-human IgG (Anti-HIgG) using N-ethyl-N’-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide chemistry. These fabricated PANI/ITO and Anti-HIgG/PANI/ITO plates have been characterized using scanning electron microscopy, cyclic voltammetry, differential pulse voltammetry and Fourier transform infra-red studies. The capacitance measurements indicate that dielectric medium of this biologically modified PPC (Anti-HIgG/PANI/ITO) is sensitive to HIgG in 5 − 5 × 105 ng mL−1 range and has lower detection limit of 1.87 ng mL−1. The observed results reveal that this Anti-HIgG modified PPC can be used as a robust, easy-to-use, reagentless, sensitive and selective immunosensor for estimation of human IgG.


Polyaniline Electrophoretic deposition Parallel plate capacitor Immunosensor 


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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Amay Jairaj Bandodkar
    • 1
  • Chetna Dhand
    • 2
  • Sunil K. Arya
    • 2
  • M. K. Pandey
    • 2
  • Bansi D. Malhotra
    • 2
    • 3
  1. 1.Department of Applied Chemistry, Institute of TechnologyBanaras Hindu UniversityVaranasiIndia
  2. 2.Department of Science and Technology Centre on Biomolecular ElectronicsNational Physical LaboratoryNew DelhiIndia
  3. 3.Centre for NanoBioEngineering and SpintronicsChungnam National UniversityDaejeonSouth Korea

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