Biomedical Microdevices

, Volume 12, Issue 1, pp 49–54 | Cite as

Fabrication of three-dimensional microarray structures by controlling the thickness and elasticity of poly(dimethylsiloxane) membrane

  • Dae Ho Lee
  • Joong Yull Park
  • Eun-Joong Lee
  • Yoon Young Choi
  • Gu Han Kwon
  • Beop-Min Kim
  • Sang-Hoon Lee
Article

Abstract

In this paper, we propose a method to construct three-dimensional curved microstructures with easy control of the size, position and shape, by exploiting the elasticity of poly(dimethylsiloxane) (PDMS) membranes and basic physics. For this end, we developed the method to handle thin PDMS membrane safely, and to replicate PDMS microstructure from the PDMS mold. Using this method, we demonstrated two potential applications: (1) the use of concave well for the formation of embryoid body (EB) to differentiate into neuronal cells, and (2) the fabrication of SU-8 and hydrogel microparticles having diverse curved shapes. The curved structures were successfully fabricated with simple process, and EBs were formed in the concave well and differentiated into the neuronal cells. Microparticles with diverse shapes were fabricated from a range of materials for potential use as drug carrier and pH responsive micro-actuator elements.

Keywords

PDMS membrane Curved microstructure Elasticity of PDMS Embryoid body 

Supplementary material

10544_2009_9357_MOESM1_ESM.pdf (673 kb)
ESM1(PDF 672 kb)

References

  1. C.F. Chen, S.D. Tzeng, H.Y. Chen, S. Gwo, Opt Lett 30, 652–654 (2005)CrossRefGoogle Scholar
  2. S.M. Dang, M. Kyba, R. Perlingeiro, G.Q. Daley, P.W. Zandstra, Biotechnol Bioeng 78, 442–453 (2002)CrossRefGoogle Scholar
  3. M. Di Giovanni, in Flat and corrugated diaphragm design handbook, ed. by M. Dekker. (New York, 1982)Google Scholar
  4. U.B. Giang, D. Lee, M.R. King, L.A. DeLouise, Lab Chip 7, 1660–1662 (2007)CrossRefGoogle Scholar
  5. Y. Hongbin, Z. Guangya, C.F. Siong, W. Shouhua, L. Feiwen, Sens Actuators B Chem 137, 754–761 (2009)CrossRefGoogle Scholar
  6. C.M. Hwang, A. Khademhosseini, Y. Park, K. Sun, S.H. Lee, Langmuir 24, 6845–6851 (2008)CrossRefGoogle Scholar
  7. K.H. Jeong, J. Kim, L.P. Lee, Science 312, 557–561 (2006)CrossRefGoogle Scholar
  8. J.M. Karp, J. Yeh, G. Eng, J. Fukuda, J. Blumling, K.Y. Suh, J. Cheng, A. Mahdavi, J. Borenstein, R. Langer, A. Khademhosseini, Lab Chip 7, 786–794 (2007)CrossRefGoogle Scholar
  9. A. Khademhosseini, J. Yeh, S. Jon, G. Eng, K.Y. Suh, J.A. Burdick, R. Langer, Lab Chip 4, 425–430 (2004)CrossRefGoogle Scholar
  10. J. Kim, J. Baek, K. Lee, Y. Park, K. Sun, T. Lee, S. Lee, Lab Chip 6, 1091–1094 (2006)CrossRefGoogle Scholar
  11. M.J. Madou, Fundamentals of Microfabrication: The Science of Miniaturization, 2nd edn. (CRC Press, Florida 2002)Google Scholar
  12. J.Y. Park, C.M. Hwang, S.H. Lee, Biomed Microdevices 11, 129–133 (2009)CrossRefGoogle Scholar
  13. M.M. Storms, R. Kormelink, D. Peters, J.W. Van Lent, R.W. Goldbach, Virology 214, 485–493 (1995)CrossRefGoogle Scholar
  14. S.L. Tao, K. Popat, T.A. Desai, Nat Protoc 1, 3153–3158 (2006)CrossRefGoogle Scholar
  15. Y.S. Torisawa, B.H. Chueh, D. Huh, P. Ramamurthy, T.M. Roth, K.F. Barald, S. Takayama, Lab Chip 7, 770–776 (2007)CrossRefGoogle Scholar
  16. M.J. Vasile, Z. Niu, R. Nassar, W. Zhang, S. Liu, J Vac Sci Technol B 15, 2350 (1997)CrossRefGoogle Scholar
  17. M.H. Wu, S.B. Huang, Z. Cui, G.B. Lee, Biomed Microdevices 10, 309–319 (2008)CrossRefGoogle Scholar
  18. Y. Xia, G.M. Whitesides, Annu Rev Mater Sci 28, 153 (1998)CrossRefGoogle Scholar
  19. S.H. Yun, C. Boudoux, G.J. Tearney, B.E. Bouma, Opt Lett 28, 1981–1983 (2003)CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Dae Ho Lee
    • 1
  • Joong Yull Park
    • 1
    • 2
  • Eun-Joong Lee
    • 1
  • Yoon Young Choi
    • 1
  • Gu Han Kwon
    • 1
  • Beop-Min Kim
    • 1
  • Sang-Hoon Lee
    • 1
  1. 1.Department of Biomedical Engineering, College of Health ScienceKorea UniversitySeoulRepublic of Korea
  2. 2.Department of Biomedical Engineering, College of EngineeringUniversity of MichiganAnn ArborUSA

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