Biomedical Microdevices

, 11:313 | Cite as

Nanoscale patterning of kinesin motor proteins and its role in guiding microtubule motility

  • Vivek Verma
  • William O. Hancock
  • Jeffrey M. Catchmark
Article

Abstract

Biomolecular motor proteins have the potential to be used as ‘nano-engines’ for controlled bioseparations and powering nano- and microelectromechanical systems. In order to engineer such systems, biocompatible nanofabrication processes are needed. In this work, we demonstrate an electron beam nanolithography process for patterning kinesin motor proteins. This process was then used to fabricate discontinuous kinesin tracks to study the directionality of microtubule movement under the exclusive influence of surface bound patterned kinesin. Microtubules moved much farther than predicted from a model assuming infinite microtubule stiffness on tracks with discontinuities of 3 μm or less, consistent with a free-end searching mechanism. As the track discontinuities exceeded 3 μm, the measured and predicted propagation distances converged. Observations of partially fixed microtubules suggest that this behavior results from the interaction of the microtubules with the surface and is not governed predominately by the microtubule flexural rigidity.

Keywords

Electron beam lithography Kinesin patterning Microtubule flexural rigidity 

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Vivek Verma
    • 1
  • William O. Hancock
    • 2
  • Jeffrey M. Catchmark
    • 3
    • 4
  1. 1.Department of Engineering Science and MechanicsPennsylvania State UniversityUniversity ParkUSA
  2. 2.Bioengineering DepartmentPennsylvania State UniversityUniversity ParkUSA
  3. 3.Agricultural and Biological Engineering Department, College of Agricultural SciencesPennsylvania State UniversityUniversity ParkUSA
  4. 4.Center for NanoCellulosics, Pennsylvania Ben Franklin Technology Partners, Center of ExcellenceThe Pennsylvania State UniversityUniversity ParkUSA

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