Abstract
A rapid and sensitive chemiluminescence microfluidic immunoassay system based on super-paramagnetic microbeads for determination of alpha-fetoprotein (AFP) is described. In this system we use CO2 laser to fabricate microfluidic chip, and use super-paramagnetic microbeads as solid carrier of antibody, chemiluminescence as detection signal. With this system we can perform AFP analysis within 20 min, and the linear range of AFP concentration is 1∼800 ng/mL, the detection limit is 0.23 ng/mL. By this method no separation or preconcentration steps are needed. Most of all, the chip can be reused.
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This work was supported by the National Natural Science Foundation of China (No. 30270407).
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Huang, H., Zheng, X.L., Zheng, J.S. et al. Rapid analysis of alpha-fetoprotein by chemiluminescence microfluidic immunoassay system based on super-paramagnetic microbeads. Biomed Microdevices 11, 213–216 (2009). https://doi.org/10.1007/s10544-008-9226-z
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DOI: https://doi.org/10.1007/s10544-008-9226-z