Use of nanoporous alumina surface for desorption electrospray ionization mass spectrometry in proteomic analysis

Abstract

This paper presents use of a nanoporous alumina surface for desorption electrospray ionization mass spectrometry (DESI MS). The DESI MS performance of the nanoporous alumina surface is compared with that of polymethylmethacrylate (PMMA), polytetrafluroethylene (PTFE) and glass, which are popular surfaces in DESI MS experiments. Optimized operating conditions were determined for each of these surfaces by studying the effects of flow rate, tip to surface and surface to MS capillary distance, and spray angle on the DESI MS performance. The analytes (reserpine and BSA tryptic digest) were analyzed on all the surfaces. The results show that the nanoporous alumina surface offers higher ion intensity and increased peptide detection as compared to the other surfaces. Additionally, comparison of ion intensities obtained from the nanoporus alumina and an alumina film confirms that improved performance is due to the inherent nature of the nanostructured surface. Limits of detection (LODs) were determined for the analytes on all the surfaces. It was observed that the nanoporous alumina surface offers improved limits of detection as compared to other surfaces. Another advantage of the nanoporous alumina surface is that it provides to faster analysis associated with rapid drying of liquid samples on the surface. Additionally, porous alumina surface can be used as a dual ionization platform for combined DESI/LDI analysis for further improved peptide detection in proteomic analysis.

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References

  1. A. Benninghoven, F.G. Rudenauer, H.W. Werner, Series of Monographs on Analytical Chemistry and Its Applications, Vol. 86 (Wiley, New York, 1987)

  2. G. Berkel, M.J. Ford, M.A. Deibel, Anal. Chem. 77, 1207–1215 (2005)

    Article  Google Scholar 

  3. I. Cotte-Rodriguez, Z. Takats, N. Talaty, H. Chen, B. Gologan, R.G. Cooks, Anal. Chem. 77, 6755 (2005)

    Article  Google Scholar 

  4. K.L. Busch, Desorption ionization mass spectrometry J. Mass Spectrom. 30, 233 (1995)

    Article  Google Scholar 

  5. R.B. Cody, J.A. Laramee, H.D. Durst, Anal. Chem. 77, 2297 (2005)

    Article  Google Scholar 

  6. R.G. Cooks, S.C. Jo, J. Green, Collisions of organic ions at surfaces Appl. Surf. Sci. 13, 231 (2004)

    Google Scholar 

  7. J.B. Fenn, M. Mann, C.K. Meng, S.F. Wong, C.M. Whitehouse, Science 246, 64 (1989)

    Article  Google Scholar 

  8. J.B. Fenn, M. Mann, C.K. Meng, S.F. Wong, C.M. Whitehouse, Mass Spectrom. Rev. 9, 37 (1990)

    Article  Google Scholar 

  9. T. Kauppila, N. Talaty, P.K. Salo, T. Kotiaho, R. Kostiainen, R.G. Cooks, Rapid Commun. Mass Spectrom. 20, 2143 (2006)

    Article  Google Scholar 

  10. L.A. Leuthold, J.F. Mandscheff, M. Fathi, C. Giroud, M. Augsburger, E. Varesio, G. Hopfgartner, Rapid Commun. Mass Spectrom. 20, 103 (2006)

    Article  Google Scholar 

  11. R. Nayak, D.R. Knapp, Anal. Chem. 79, 4950 (2007)

    Article  Google Scholar 

  12. R. Nayak, A.K. Sen, D.R. Knapp, J. Liu, J. Am. Soc. Mass Spectrom. (2008) (in press).

  13. M. Nefliu, A. Venter, R.G. Cooks, C. Moore, J. Am. Soc. Mass Spectrom., 17, 1091 (2006)

    Article  Google Scholar 

  14. N. Talaty, Z. Takats, R.G. Cooks, Analyst, 130, 1624 (2005)

    Article  Google Scholar 

  15. S.E. Rodriguez-Cruz, Rapid Commun. Mass Spectrom. 20, 53 (2006)

    Article  Google Scholar 

  16. Z. Takats, J.M. Wiseman, B. Golagan, R.G. Cooks, Science 306, 471 (2004)

    Article  Google Scholar 

  17. Z. Takats, I. Cotte-Rodriguez, N. Talaty, H. Chen, R.G. Cooks, Chem. Commun. 15, 1950 (2005)

    Article  Google Scholar 

  18. D.J. Weston, R. Bateman, I.D. Wilson, T.R. Wood, C.S. Creaser, Anal. Chemistry, 77. (2005)

  19. J.P. Williams, J.H. Scrivens, Rapid Commun. Mass Spectrom. 19(3), 364 (2005)

    Google Scholar 

  20. N. Winograd, Appl. Surf. Sci. 203, 13 (2003)

    Article  Google Scholar 

  21. J.M. Wiseman, S.M. Puolitaival, Z. Takats, R.G. Cooks, R.M. Caprioli, Angew. Chem. Int. Ed. 41, 7094 (2005)

    Article  Google Scholar 

Download references

Acknowledgement

This work was supported in part by NIH-NCI grant CA 86285 and the NIH NHLBI Proteomics Initiative via contract N01-HV-28181.

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Correspondence to J. Darabi.

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Sen, A.K., Nayak, R., Darabi, J. et al. Use of nanoporous alumina surface for desorption electrospray ionization mass spectrometry in proteomic analysis. Biomed Microdevices 10, 531–538 (2008). https://doi.org/10.1007/s10544-007-9162-3

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Keywords

  • Nanoporous alumina
  • Desorption electrospray ionization
  • Polymethylmethacrylate
  • Polytetrafluroethylene
  • Limits of detection