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Fabrication and evaluation of microgrooved polymers as peripheral nerve conduits

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Abstract

Cell alignment plays an important role in the repair of damaged peripheral nerves. The aligned Schwann cells could direct the axonal outgrowth during nerve reconstruction. One way of aligning Schwann cells is to use surface grooves in micrometric dimensions. In this study, microgrooves on chitosan or poly(d,l-lactide) (PLA) were fabricated and the behaviors of Schwann cells and glial cell line C6 on these surfaces were examined. It was found that Schwann cells and C6 cells could be successfully aligned by the microgrooves, and express the genes related to the production of neurotrophic factors. The polymer conduits with microgrooves on the inner surface were implanted in rats to repair the damaged sciatic nerve. The microgrooved conduits were demonstrated to enhance peripheral nerve regeneration as compared to the smooth conduits.

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Acknowledgments

This work was supported by grants from National Chung Hsing University-Taichung Veterans General Hospital, National Science Council and National Health Research Institutes. The study was conducted in the Center of Tissue Engineering and Stem Cells Research, National Chung Hsing University. The corresponding author is jointly appointed by the Center of Nanoscience and Nanotechnology of the university. Part of the silicon photolithography was kindly provided by Professor C-S. Lai of Chang Gung University.

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Authors and Affiliations

  1. Department of Chemical Engineering, National Chung Hsing University, 250 Kuo Kuang Road, Taichung, 402, Taiwan, Republic of China

    Shan-hui Hsu, Po Seng Lu, Hsiao-Chiang Ni & Chien-Hsiang Su

  2. Institute of Biomedical Engineering, National Chung Hsing University, Taichung, Taiwan, Republic of China

    Shan-hui Hsu

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  1. Shan-hui Hsu
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  2. Po Seng Lu
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  3. Hsiao-Chiang Ni
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  4. Chien-Hsiang Su
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Correspondence to Shan-hui Hsu.

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Hsu, Sh., Lu, P.S., Ni, HC. et al. Fabrication and evaluation of microgrooved polymers as peripheral nerve conduits. Biomed Microdevices 9, 665–674 (2007). https://doi.org/10.1007/s10544-007-9068-0

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