Abstract
With an advanced computational fluid dynamics (CFD) technique, we have numerically developed and examined a micro-biolistic system for delivering particles to murine target sites. The micro-particles are accelerated by a high speed flow initiated by a traveling shock wave, so that they can attain a sufficient momentum to penetrate in to the cells of interest within murine skin (or mucosa). In immunization application, powdered vaccines are directly delivered into the antigen presenting cells (APCs) within the epidermis/dermis of the murine skin with a narrow and highly controllable velocity range (e.g., 699±5.6 m/s for 1.8 μm modeled gold particles) and a uniform spatial distribution over a diameter of ~ 4 mm target area. Key features of gas dynamics and gas-particle interaction are presented. Importantly, the particle impact velocity conditions are quantified as a function of: stand-off distance (2–15 mm), driver gas species (air/helium mixtures), particle density (1,050 kg/m3 and 19,320 kg/m3) and particle size (1–5 μm for gold particles and 10–50 μm for less dense particles, respectively). The influential parameters—representative of immunotherapeutic (e.g., DNA vaccination) and protein (e.g., lidocaine) biolistic applications—are studied in detail.
Similar content being viewed by others
References
B.J. Bellhouse, D.F. Sarphie, J.C. Greenford, Int. Patent WO94/24263, 1994
A.M. Bennett, R.J. Phillpotts, S.D., Perkins, S.C. Jacobs, E.D. Williamson, Vaccines 18(7–8), 588–596 (1999)
Bio-Rad Laboratories, Hercules, CA 94547, USA. (also see, http://www.bio-rad.com/)
D. Chen , Y. Maa, J.R. Haynes, Expert Review of Vaccines, 1(3), 89–100 (2002)
R.F. Chisnell, J. Fluid Mech. 2, 286–298 (1957)
J. Dileo, T.E. Miller Jr., L. Huang, Hum. Gene Ther. 14(1), 79–87 (2003)
Fluent Inc., Fluent user’s guide volume, Lebanon, NH 03766, USA (also see, http://www.fluent.com/)
R. Han , X. Peng, C.A. Reed, N.M. Cladel, L.R. Budgeon, M.D. Pickel, N.D. Christensen, Cancer Detect. Prev. 26(6), 458–467 (2002)
M.P. Hardy, M.A.F. Kendall, Phys. Med. Biol. 50(19), 4567–4580 (2005)
O. Igra, K. Takayama, Proc. R. Soc. A: Math. Phys. Eng. Sci. 442, 231–2467 (1993)
S. Kamstrup, A.M. Barfoed, T.H. Frimann, A. Ladekjær-Mikkelsen, A. Btner, Vaccines 22, 1358–1361 (2004)
M.A.F. Kendall, Shock Waves J. 12(1), 23–30 (2002)
M.A.F. Kendall, T.J. Mitchell, P. Wrighton-Smith, J. Biomech. 37(11), 1733–1741 (2004a)
M.A.F. Kendall, S. Rishworth, F.V. Carter, T.J. Mitchell, J. Invest. Dermatol. 122(3), 739–746 (2004b)
M.A.F. Kendall, N.J. Quinlan, S.J. Thorpe, R.W. Ainsworth, B.J. Bellhouse, Exp. Fluids 37(1), 128–136 (2004c)
T.M. Klein, E.D. Wolf, R. Wu, J.C. Sanford, Nature 327, 70–73 (1987)
M.T. Lin, L. Pulkkinen, J. Uitto, K. Yoon, J. Invest. Dermatol. 39(3), 161–70 (2000)
Y. Liu, M.A.F. Kendall, Proc. Inst. Mech. Eng., Part C-J. Mech. Eng. Sci. 218(11), 1373–1383 (2004)
Y. Liu, B.J. Bellhouse, Int. J. Numer. Methods Fluids 47(10–11), 1147–1155 (2005)
Y. Liu, Med. Biol. Eng. Comput. 44(7), 551–559 (2006a)
Y. Liu, M.A.F. Kendall, Biomed. Microdevices 8(4), 341–351 (2006b)
Y. Liu, Med. Eng. Phys. 29(3), 390–397 (2007a)
Y. Liu, M.A.F. Kendall, Comput. Meth. Prog. Bio. 85(2), 124–128 (2007b)
M.C. Marrion, M.A.F. Kendall, Y. Liu, Exp. Fluids 28(3), 319–327 (2005)
T.J. Mitchell, M.A.F. Kendall, B.J. Bellhouse, Int. J. Impact Eng. 28, 581–599 (2003)
W.J. Mulholland, M.A.F. Kendall, N. White, B.J. Bellhouse, Phys. Med. Biol. 49, 5043–5058 (2004)
H. Qin, C. Zhou, D. Wang, W. Ma, X. Liang, C. Lin, Y. Zhang, S. Zhang, Immunol. Lett. 99(1), 85–93 (2005)
P.A. Raju, N. McSloy, N.K. Truong, M.A.F. Kendall, Vaccines 24(21), 4644–4647 (2006)
D. Rinberg, C. Simonnet, A. Groisman, Appl. Phys. Lett. 87(1), 014103 (2005)
C.E. Smith, J. Fluid Mech. 24, 625–641 (1966)
C. Trimble, C. Lin, C. Hung, S. Pai, J. Juang, L. He, M. Gillison, D. Pardoll, L. Wu, T.C. Wu, Vaccines 21(25–26), 4036–4042 (2003)
N. K. Truong, Y. Liu, M.A.F. Kendall, Shock Waves J. 15(3–4), 149–164 (2006)
V.B. Vassilev, L.H.V.G. Gil, R.O. Donis, Vaccines 19(15–16), 2012–2019 (2001)
A.K. Zuber, A. Bråve, G. Engström, B. Zuber, K. Ljungberg, M. Fredriksson, R. Benthin, M.G. Isaguliants, E. Sandström, J. Hinkula, B. Wahren, Vaccines 22(13–14), 1791–1798 (2004)
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Liu, Y., Truong, N.K., Kendall, M.A.F. et al. Characteristics of a micro-biolistic system for murine immunological studies. Biomed Microdevices 9, 465–474 (2007). https://doi.org/10.1007/s10544-007-9053-7
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10544-007-9053-7