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Microdevice-based delivery of gene products using sonoporation

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Abstract

This paper presents a proof-of-concept miniature device for delivery of antisense oligonucleotides (ASO). A piezoelectric, lead zirconate titanate (PZT) plate (0.5 cm2 × 0.75 mm) is used to transfect cells using cavitation-induced sonoporation. Both human umbilical vein endothelial cells (HUVEC) and human prostate cancer cells (PC3) are investigated in vitro. Preliminary results show that after sonication, the transfection rate for HUVEC increases by 96% compared to controls (p < 0.01). For PC3, the transfection rate increases by 31% compared to controls (p < 0.02). This research can potentially be applied in realizing a microelectromechanical system (MEMS)-based device for gene therapy in cancer treatment.

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Authors and Affiliations

  1. Department of Mechanical Engineering, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada

    Tung Siu & Mu Chiao

  2. Department of Mechanical Engineering and Department of Electrical and Computer Engineering, University of British Columbia, Vancouver, BC V6T 1Z4, Canada

    Robert Rohling

Authors
  1. Tung Siu
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  2. Robert Rohling
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  3. Mu Chiao
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Corresponding author

Correspondence to Mu Chiao.

Additional information

Part of this paper was presented at the 13th International Conference on Solid-State Sensors and Actuators (Transducers’ 05), June, 2005.

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Siu, T., Rohling, R. & Chiao, M. Microdevice-based delivery of gene products using sonoporation. Biomed Microdevices 9, 295–300 (2007). https://doi.org/10.1007/s10544-006-9028-0

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  • DOI: https://doi.org/10.1007/s10544-006-9028-0

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