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Transcription Factor GATA-3 Is Involved in Repression of Promoter Activity of the Human Interleukin-4 Gene

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Abstract

GATA-3 was shown to bind to two sites of the IL-4 gene promoter in human T-cell lines PER-117 and Jurkat. A motif located in the region of position-860 and responsible for GATA-3 binding was detected for the first time. Mutation or deletion of this site increased the promoter activity. The findings suggest a direct involvement of GATA-3 in regulation of the human IL-4 gene transcription as a repressor of the promoter activity.

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Abbreviations

PMA:

phorbol 12-myristate 13-acetate

CaI:

calcium ionophore

αCD28:

antibodies to CD28 (α-Leu-CD28)

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Authors and Affiliations

  1. Curtin University School of Biomedical Sciences and the Western Australian Institute of Medical Research, Molecular Immunology Group, Level 5 Rear 50 Murray Street, Perth, 6000, Western Australia

    G. T. F. Schwenger & C. J. Sanderson

  2. Institute of Cytology and Genetics, Siberian Division, Russian Academy of Sciences, pr. Lavrentieva 10, 630090, Novosibirsk, Russia

    V. A. Mordvinov

Authors
  1. G. T. F. Schwenger
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  2. V. A. Mordvinov
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  3. C. J. Sanderson
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Corresponding author

Correspondence to V. A. Mordvinov.

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__________

Translated from Biokhimiya, Vol. 70, No. 9, 2005, pp. 1289–1294.

Original Russian Text Copyright © 2005 by Schwenger, Mordvinov, Sanderson.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM05-092, July 24, 2005.

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Schwenger, G.T.F., Mordvinov, V.A. & Sanderson, C.J. Transcription Factor GATA-3 Is Involved in Repression of Promoter Activity of the Human Interleukin-4 Gene. Biochemistry (Moscow) 70, 1065–1069 (2005). https://doi.org/10.1007/s10541-005-0225-x

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  • DOI: https://doi.org/10.1007/s10541-005-0225-x

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