Abstract
Thermostable dimeric malate dehydrogenase (MDH) was isolated from the microorganism of hydrothermal vents Vulcanithermus medioatlanticus. The enzyme was electrophoretically homogeneous and possessed the specific activity of 6.9 U/mg. The large molecular weight of the subunits (55 kD) is likely to provide the rigidity of the enzyme structure (the activation energy of the enzymatic reaction is 32.6 kJ/mol). The thermophilic MDH differs little from the mesophilic enzyme in terms of kinetic and regulatory characteristics.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Vieille, C., Burdette, D. S., and Zeikus, J. G. (1996) Biotechnol. Annu. Rev., 2, 1–83.
Gromiha, M. M. (2001) Biophys. Chem., 91, 71–77.
Fukuchi, S., and Nishikawa, K. (2001) J. Mol. Biol., 309, 835–843.
Scandurra, R., Consalvi, V., Chiaraluce, R., Politi, L., and Engel, P. C. (1998) Biochimie, 80, 933–941.
Sterner, R., and Liebl, W. (2001) Crit. Rev. Biochem. Mol. Biol., 36, 39–106.
Hochachka, P., and Somero, G. (1988) in Biochemical Adaptation [Russian translation], Mir, Moscow.
Dalhus, B., Saarinen, M., Sauer, U. H., Eklund, P., Jahansson, K., Karlsson, A., Ramaswamy, S., Bjork, A., Syndtas, B., Naterstad, K., Sirevag, R., and Eklund, H. (2002) J. Mol. Biol., 318, 707–721.
Sundarum, T. K., Wright, I. P., and Wilkinson, A. E. (1980) Biochemistry, 19, 2017–2022.
Wynne, S. A., Nicholls, D. J., Scawen, M. D., and Sundaram, T. K. (1996) Biochem. J., 317, 235–245.
Smith, K., and Sundaram, T. K. (1988) Biochim. Biophys. Acta, 955, 203–213.
Eprintsev, A. T., Falaleeva, M. I., Stepanova, I. Yu., and Parfyonova, N. V. (2003) Biochemistry (Moscow), 68, 172–176.
Eprintsev, A. T., Falaleeva, M. I., Grabovich, M. Yu., Parfyonova, N. V., Kashirskaya, N. N., and Dubinina, G. A. (2004) Mikrobiologiya, 73, 1–6.
Miroschnichenko, M. L., Haridon, S. L., Nercessian, O., Antipov, A. N., Kostrikina, N. A., Tindall, B. J., Schumann, P., Spring, S., Stackebrandt, E., Bonch-Osmolovskaya, E. A., and Jeanthion, C. (2003) Int. J. Syst. Evol. Microbiol., 53, 1143–1148.
Lowry, O. H., Roserbrough, H. J., Farr, A. L., and Randall, R. J. (1951) J. Biol. Chem., 193, 265–275.
Eprintsev, A. T., Falaleeva, M. I., Stepanova, I. Yu., Parfyonova, N. V., and Zuzu, M. (2003) Izv. Ros. Akad. Nauk. Ser. Biol., 3, 307–311.
Honka, E., Fabry, S., Niermann, T., Palm, P., and Hensel, R. (1990) Eur. J. Biochem., 188, 623–632.
Labrou, N. E., and Clonis, Y. D. (1977) Arch. Biochem. Biophys., 337, 103–114.
Grossebuter, W., Hartl, T., Gorisch, H., and Stezowski, J. J. (1986) Biol. Chem. Hoppe-Seyler, 367, 457–463.
Pineiru de Korval'iu, M. A. A., Zemlyanukchin, A. A., and Eprintsev, A. T. (1991) VGU Publisher, Voronezh.
Tayeh, M. A., and Madigan, M. T. (1988) Biochem. J., 252, 595–600.
Tyagi, A. K., Siddiqui, F. A., and Vtnkitasubramanian, T. A. (1977) Biochim. Biophys. Acta, 485, 255–267.
Irimia, A., Vellieux, F. M., Madern, D., Zaccai, G., Karshikoff, A., Tibbelin, G., Ladenstein, R., Lien, T., and Birkeland, N. K. (2004) J. Mol. Biol., 335, 343–356.
Rolstand, A. K., Howland, E., and Sirenag, R. (1988) J. Bacteriol., 170, 2947–2953.
Ohshima, T., and Tanaka, S. (1993) Eur. J. Biochem, 214, 37–42.
Kristjansson, H., and Ponnamperuma, C. (1980) Orig. Life, 10, 185–192.
Yueh, A. Y., Chung, C. S., and Lai, Y. K. (1989) Biochem. J., 258, 221–228.
Author information
Authors and Affiliations
Corresponding author
Additional information
__________
Translated from Biokhimiya, Vol. 70, No. 9, 2005, pp. 1245–1249.
Original Russian Text Copyright © 2005 by Eprintsev, Falaleeva, Parfyonova.
Rights and permissions
About this article
Cite this article
Eprintsev, A.T., Falaleeva, M.I. & Parfyonova, N.V. Malate Dehydrogenase from the Thermophilic Bacterium Vulcanithermus medioatlanticus . Biochemistry (Moscow) 70, 1027–1030 (2005). https://doi.org/10.1007/s10541-005-0220-2
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/s10541-005-0220-2