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Metabolic Activity of Macrophages Infected with Hantavirus, an Agent of Hemorrhagic Fever with Renal Syndrome

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Abstract

Monocytes/macrophages are thought to play an important role in pathogenesis of viral infections. These cells are involved in distribution and persistence of viruses in the organism and also influence the regulation of immune reactions. The functional and enzymatic activities of macrophages infected with an agent of hemorrhagic fever with renal syndrome were analyzed for the first time. This disease is caused by a virus of the Hantavirus genus, the Bunyaviridae family. Activities of ectoenzymes 5′-nucleotidase and ATPase of the plasma membrane of the hantavirus infected macrophages decreased along with the antigen accumulation in the infected cells. The contact of phagocytes with hantavirus-resulted in activation in the cells of the oxygen-dependent metabolism and NO-synthase. The NO-synthase-dependent system of the infected macrophages was activated earlier than their oxygen-dependent system. The intracellular contents of acid and alkaline phosphatases increased within the first hours after the infection. The bactericidal activity of the hantavirus-infected macrophages relatively to Staphylococcus aureus increased during the specific antigen accumulation in the phagocytes. Thus, the infection of macrophages with hantavirus was associated with intracellular metabolic changes.

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REFERENCES

  1. Cotter, R. L., Zheng, J., Che, M., Niemann, D., Liu, Y., He, J., Thomas, E., and Gendelman, H. E. (2001) J. Virol., 75, 4308–4320.

    Article  PubMed  Google Scholar 

  2. Totolyan, A. A., and Freidlin, I. S. (2000) The Immune System Cells [in Russian], Nauka, St. Petersburg.

    Google Scholar 

  3. Shevak, I. M. (1987) in Immunology (Pol, U., ed.) Vol. 1, Mir, Moscow, pp. 164–166.

  4. Diakonova, M., Bokoch, G., and Swanson, J. A. (2002) Mol. Biol. Cell., 13, 402–411.

    Article  PubMed  Google Scholar 

  5. Schimmel, S. D., Kent, C., Bischoff, R., and Vagelos, P. R. (1973) Proc. Natl. Acad. Sci. USA, 70, 3195–3199.

    PubMed  Google Scholar 

  6. Misumi, Y., Ogata, S., Hicose, S., and Ikehara, Y. (1990) J. Biol. Chem., 265, 2178–2183.

    PubMed  Google Scholar 

  7. Zimmermann, H. (1992) Biochem. J., 285, 345–365.

    PubMed  Google Scholar 

  8. Kirilicheva, G. B., Naumov, A. V., Taranenko, T. M., Solov'eva, M. S., and Andreeva, I. P. (1992) Byull. Eksp. Biol. Med., 3, 297–299.

    Google Scholar 

  9. Mit'kin, V. V., Volkov, N. I., Pshenichnikova, T. Ya., and Sukhikh, T. G. (1992) Byull. Eksp. Biol. Med., 2, 51–54.

    Google Scholar 

  10. Dawson, R. M. C., Elliott, D. C., Elliot, W. H., and Jones, K. M. (1986) Data for Biochemical Research, Oxford.

  11. Dubinina, E. E. (1989) Usp. Sovr. Biol., 108, 3–18.

    Google Scholar 

  12. Pokrovskii, V. I., and Nagoev, B. S. (1983) The NBT-Test for Neutrophil Leukocytes and Its Clinical Significance, Nal'chik.

  13. Nathan, C. (1997) J. Clin. Invest., 100, 2417–2423.

    PubMed  Google Scholar 

  14. Webb, J. L., Harvey, M. W., Holden, D. W., and Evans, T. J. (2001) Infect. Immun., 69, 6391–6400.

    Article  PubMed  Google Scholar 

  15. Gorren, A. C. F., and Mayer, B. (1998) Biochemistry (Moscow), 63, 734–743.

    Google Scholar 

  16. Loida, Z., Gossrau, P., and Shibler, T. (1982) Enzyme Histochemistry. Laboratory Methods [Russian translation], Mir, Moscow.

  17. Savard, M., Belanger, C., Tardif, M., Gourde, P., Flamand, L., and Gosselin, J. (2000) J. Virol., 74, 2612–2619.

    Article  PubMed  Google Scholar 

  18. Thomas, C. A., Weinberger, O. K., Ziegler, B. L., Greenberg, S., Schieren, I., Silverstein, S. C., and Khoury, J. El. (1997) Blood, 90, 3760–3765.

    PubMed  Google Scholar 

  19. Li, Kh. V. (2003) in Hantaviruses and Hantaviral Infections (Slonov, R. A., and Ivanis, V. A., eds.) [in Russian], Vladivostok, pp. 42–56.

  20. Plyusnin, A., Vapalahti, O., and Vaheri, A. (1996) J. Gen. Virol., 77, 2677–2687.

    PubMed  Google Scholar 

  21. Marcotic, A., Rabatic, S., Gagro, A., Sabioncello, A., Kuzman, I., and Dekaris, D. (1999) in Factors in Emergence and Control of Rodent Borne Viral Diseases (Hantaviral and Arenal Diseases) (Saluzzo, J. F., and Dodet, B., eds.) pp. 125–131.

  22. Yao, J. S., Arikawa, J., Kariva, H., Yoshimatsu, K., Takashima, I., and Hashimoto, N. (1992) Jpn. J. Vet. Res., 40, 87–97.

    PubMed  Google Scholar 

  23. Nagai, T., Tanishita, O., Takahashi, Y., Yamanouchi, T., Domae, K., Kondo, K., Dantas, J. R., Takahashi, Jr., M., and Yamanishi, K. (1985) J. Gen. Virol., 66, 1271–1278.

    PubMed  Google Scholar 

  24. Laboratory Diagnosis of Hemorrhagic Fever with Renal Syndrome. Methodical Recommendations (1982), Moscow.

  25. Takenaka, A., Gibbs, C. J., and Gajdusek, D. C. (1985) Arch. Virol., 84, 197–206.

    Article  PubMed  Google Scholar 

  26. Kirillicheva, G. B., Kirillichev, A. A., and Tumanyan, M. A. (1988) in Immunomodifiers in Infectious Pathology [in Russian], Gamaleya Institute of Experimental Medicine, USSR Academy of Medical Sciences, Moscow, pp. 55–56.

    Google Scholar 

  27. Butakov, A. A. (1991) Elaboration of Express Assessment of the Phagocytic Component of Immunity for Immune Epidemiology: Author's abstract of candidate's dissertation [in Russian], Institute of Immunology, Moscow.

    Google Scholar 

  28. Hope, B. T., and Vinsent, S. R. (1989) J. Histochem. Cytochem., 37, 653–661.

    PubMed  Google Scholar 

  29. Pigarevskii, V. E., and Mazing, Yu. A. (1985) A Search for and Development of New Tests for Diagnosis of Nonspecific Resistance: Conclusion of the Institute of Experimental Medicine, Academy of Medical Sciences, St. Petersburg.

    Google Scholar 

  30. Hayhoe, F. G. J., and Quaglino, D. (1983) Hematological Cytochemistry [Russian translation], Meditsina, Moscow.

    Google Scholar 

  31. Garlinski, P., Mlynarczyk, G., Roszkowski, W., Pulverer, G., and Jeljaszewicz, A. (1987) J. Zbl. Bact. Hyg., A 266, 43–51.

    Google Scholar 

  32. Kislyak, N. S., and Lenskaya, R. V. (1978) Blood Cells in Children in Health and Disease [in Russian], Meditsina, Moscow.

    Google Scholar 

  33. Kirilicheva, G. B., Solov'eva, M. S., Baturina, I. G., Shchukovskaya, T. N., and Maslennikov, G. A. (1992) Byull. Eksp. Biol. Med., 3, 296–297.

    Google Scholar 

  34. Conboy, I. M., Manoli, D., Mhaiskar, V., and Jones, P. P. (1999) Proc. Natl. Acad. Sci. USA, 96, 6324–6329.

    Article  PubMed  Google Scholar 

  35. Mayanskii, A. N., and Mayanskii, D. N. (1989) Essays about Neutrophil and Macrophage [in Russian], Nauka, Novosibirsk.

    Google Scholar 

  36. Nathan, C., and Shiloh, M. U. (2000) Proc. Natl. Acad. Sci. USA, 97, 8841–8848.

    Article  PubMed  Google Scholar 

  37. Pope, M., Marsden, P. A., Cole, E., Sloan, S., Fung, L. S., Ning, Q., Ding, J. W., Leibowitz, J. L., Phillips, M. J., and Levy, G. A. (1998) J. Virol., 72, 7084–7090.

    PubMed  Google Scholar 

  38. Gonzalez-Scarano, F., and Nathanson, N. (1996) in Virology (Fields, B. N., Knipe, M. D., and Howley, P. M., eds.) 3rd Edn., Vol. 1, Lippincott Raven, Philadelphia, pp. 1473–1504.

    Google Scholar 

  39. Temonen, M., Vapalahti, O., Holthofer, H., Brummer-Korvenkontio, M., Vaheri, A., and Lankinen, H. (1995) J. Gen. Virol., 74, 515–518.

    Google Scholar 

  40. Pensiero, M. N., Sharefkin, J. B., Dieffenbach, C. W., and Hay, J. (1992) J. Virol., 66, 5929–5936.

    PubMed  Google Scholar 

  41. Plekhova, N. G., Somova-Isachkova, L. M., Slonova, R. A., Kompanets, G. G., Pustovalov, E. V., and Drobot, E. I. (2003) Tsitologiya, 45, 770–779.

    Google Scholar 

  42. Zemskov, V. M., Nikolaeva, E. N., and Rodionov, S. V. (1993) Usp. Sovr. Biol., 113, 336–350.

    Google Scholar 

  43. Choukroun, G. J., Marshansky, V., Gustafson, C. E., McKee, M., Hajjar, R. J., Rosenzweig, A., Brown, D., and Bonventre, J. V. (2000) J. Clin. Invest., 106, 983–993.

    PubMed  Google Scholar 

  44. Wintroub, B. V. (1982) in Advances in Inflammation (Weisman, G., ed.) Vol. 4, New York, pp. 131–136.

  45. Golubev, D. B. (1968) Enzymatic Changes in Viral Reproduction [in Russian], Meditsina, Leningrad.

    Google Scholar 

  46. Vorob'ev, L. I. (2002) in Guidebook on Hematology (Vorob'ev, L. I., ed.) [in Russian], Nyudiamed, Moscow, pp. 136–145.

    Google Scholar 

  47. Kim, S. A., Vacratsis, P. O., Firestein, R., Cleary, M. L., and Dixon, J. E. (2003) Proc. Natl. Acad. Sci. USA, 100, 4492–4497.

    Article  PubMed  Google Scholar 

  48. MacMicking, J., Xie, Q., and Nathan, C. (1997) Annu. Rev. Immunol., 15, 323–350.

    Article  PubMed  Google Scholar 

  49. Blond, D., Raoul, H. R., Grand, L., and Dormont, D. J. (2000) J. Virol., 74, 8904–8912.

    Article  PubMed  Google Scholar 

  50. Lin, Y. L., Huang, Y. L., Ma, S. H., Yeh, C. T., Chiou, S. Y., Chen, L. K., and Liao, C. L. (1997) J. Virol., 71, 5227–5235.

    PubMed  Google Scholar 

  51. Wu, L., and Morahan, P. S. (1992) Curr. Top. Microbiol., 179, 89–110.

    Google Scholar 

  52. Al'stein, A. D. (1982) in General and Special Virology (Zhdanov, V. M., and Gaidamovich, S. Ya., eds.) [in Russian], Meditsina, Moscow.

    Google Scholar 

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Authors and Affiliations

  1. Institute of Epidemiology and Microbiology, Siberian Division, Russian Academy of Medical Sciences, ul. Sel'skaya 1, 690087, Vladivostok, Russia

    N. G. Plekhova, L. M. Somova, R. A. Slonova, G. G. Companets, V. V. Luk'yanova & N. V. Yakubovich

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  1. N. G. Plekhova
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  2. L. M. Somova
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  3. R. A. Slonova
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  5. V. V. Luk'yanova
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Translated from Biokhimiya, Vol. 70, No. 9, 2005, pp. 1198–1208.

Original Russian Text Copyright © 2005 by Plekhova, Somova, Slonova, Companets, Luk'yanova, Yakubovich.

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Plekhova, N.G., Somova, L.M., Slonova, R.A. et al. Metabolic Activity of Macrophages Infected with Hantavirus, an Agent of Hemorrhagic Fever with Renal Syndrome. Biochemistry (Moscow) 70, 990–997 (2005). https://doi.org/10.1007/s10541-005-0214-0

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  • DOI: https://doi.org/10.1007/s10541-005-0214-0

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