Skip to main content
SpringerLink
Log in

Characterization of Molybdenum-Free Nitrate Reductase from Haloalkalophilic Bacterium Halomonas sp. Strain AGJ 1-3

  • Published:
Biochemistry (Moscow) Aims and scope Submit manuscript

Abstract

Nitrate reductase from the haloalkalophilic denitrifying bacterium Halomonas sp. Strain AGJ 1-3 was isolated and purified to homogeneity. The isolated enzyme belongs to a novel family of molybdenum-free nitrate reductases. It presents as a 130–140 kD monomeric protein with specific activity of 250 µmol/min per mg protein. The enzyme reduces not only nitrate, but also other anions, thus showing polyoxoanion reductase activity. Enzyme activity was maximal at pH 7.0 and 70–80°C.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. Richardson, D. J., Berks, B. C., Russell, D. A., Spiro, S., and Taylor, C. J. (2001) Cell Mol. Life Sci., 58, 165–178.

    PubMed  Google Scholar 

  2. Campbell, W. H. (2001) Cell Mol. Life Sci., 58, 194–204.

    PubMed  Google Scholar 

  3. Stolz, J. F., and Basu, P. (2002) ChemBioChem., 3, 198–206.

    Article  PubMed  Google Scholar 

  4. Moreno-Vivian, C., Cabello, P., Martinez-Luque, M., Blasco, R., and Castillo, F. (1999) J. Bacteriol., 181, 6573–6584.

    PubMed  Google Scholar 

  5. Philippot, L., and Hojberg, O. (1999) Biochim. Biophys. Acta, 1446, 1–23.

    PubMed  Google Scholar 

  6. Zumft, W. G. (1997) Microbiol. Mol. Biol. Rev., 61, 553–616.

    Google Scholar 

  7. Yoshimatsu, K., Sakurai, T., and Fujiwara, T. (2000) FEBS Lett., 470, 216–220.

    Article  PubMed  Google Scholar 

  8. Afshar, S., Jonson, E., de Vries, S., and Schroder, I. (2001) J. Bacteriol., 183, 5491–5495.

    Article  PubMed  Google Scholar 

  9. L’vov, N. P. (1989) Molybdenum in Nitrogen Assimilation in Plants and Microorganisms. 43rd Bach Meeting [in Russian], Nauka, Moscow.

    Google Scholar 

  10. Deng, M., Moureax, T., and Caboshe, M. (1989) Plant Physiol., 91, 304–309.

    Google Scholar 

  11. Antipov, A. N., Lyalikova, N. N., Khijniak, T. V., and L’vov, N. P. (1998) FEBS Lett., 441, 257–260.

    Article  PubMed  Google Scholar 

  12. Sorokin, D. Y., and Mityushina, L. L. (1998) Mikrobiologiya, 67, 93–101.

    Google Scholar 

  13. Sorokin, D. Y. (2003) Mikrobiologiya, 72, 725–739.

    Google Scholar 

  14. Pfennig, N., and Lippert, K. (1966) Arch. Microbiol., 55, 245–256.

    Google Scholar 

  15. Garret, R. H., and Nason, A. (1969) J. Biol. Chem., 244, 2870–2882.

    PubMed  Google Scholar 

  16. Hawkes, T. R., and Bray, R. C. (1984) Biochem. J., 219, 481–493.

    PubMed  Google Scholar 

  17. Davis, B. J. (1965) Ann. N. Y. Acad. Sci., 121, 404–407.

    Google Scholar 

  18. Laemmli, U. (1970) Nature, 227, 680–683.

    PubMed  Google Scholar 

  19. Nesterenko, M. V. (1994) Biochim. Biophys. Meth., 28, 231–242.

    Google Scholar 

  20. Afshar, S., Kim, C., Monbouquette, H. G., and Schroder, I. (1998) Appl. Environ. Microbiol., 64, 3004–3008.

    PubMed  Google Scholar 

  21. Antipov, A. N., Lyalikova, N. N., Khijniak, T. V., and L’vov, N. P. (1999) Biochemistry (Moscow), 64, 483–487.

    Google Scholar 

  22. Antipov, A. N., Sorokin, D. Y., L’vov, N. P., and Kuenen, J. G. (2003) Biochem. J., 369, 185–189.

    Article  PubMed  Google Scholar 

  23. Horikoshi, K. (1999) Microbiol. Mol. Biol. Rev., 63, 735–750.

    PubMed  Google Scholar 

  24. Duckworth, A. W., Grant, W. D., Jones, B. E., Meijer, D., Marquez, M. C., and Ventosa, A. (2000) Extremophiles, 4, 53–60.

    PubMed  Google Scholar 

  25. Murillo, M. F., Gugliuzza, T., Senko, J., Basu, P., and Stolz, J. F. (1999) Arch. Microbiol., 172, 313–320.

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Bach Institute of Biochemistry, Russian Academy of Sciences, Leninsky pr. 33, 119071, Moscow, Russia

    A. N. Antipov, E. V. Morozkina, L. I. Golubeva, R. A. Zvyagilskaya & N. P. L’vov

  2. Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7/2, 117811, Moscow, Russia

    D. Yu. Sorokin

Authors
  1. A. N. Antipov
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. V. Morozkina
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. Yu. Sorokin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. L. I. Golubeva
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. R. A. Zvyagilskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. N. P. L’vov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. N. Antipov.

Additional information

__________

Translated from Biokhimiya, Vol. 70, No. 7, 2005, pp. 968–973.

Original Russian Text Copyright © 2005 by Antipov, Morozkina, Sorokin, Golubeva, Zvyagilskaya, L’vov.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM04-165, February 13, 2005.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Antipov, A.N., Morozkina, E.V., Sorokin, D.Y. et al. Characterization of Molybdenum-Free Nitrate Reductase from Haloalkalophilic Bacterium Halomonas sp. Strain AGJ 1-3. Biochemistry (Moscow) 70, 799–803 (2005). https://doi.org/10.1007/s10541-005-0186-0

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10541-005-0186-0

Key words

Search

Navigation