Abstract
Resting naive CD4+CD45R0−CD45RA+ T cells are sensitive to ionomycin. In contrast, resting CD4+CD45RA−CD45R0+ memory T cells show resistance to this Ca2+ ionophore. In the present study, the ability of activated T lymphocytes to respond to ionomycin during the transition from naive precursors into memory T cells has been analyzed. Activated CD4+CD45RA+CD45R0+ T cells are always present both in human peripheral blood (HPB) and in the ionomycin-resistant (IR) fraction. Therefore, some activated T cells are resistant toward the Ca2+ ionophore. CD69 molecules are markers of the very early stage of T cell activation. However, CD4+CD69+ T cells have never been found in the IR fraction. Thus, the majority of CD4+ T lymphocytes at the early stage of activation are ionomycin-sensitive cells. The proportion of CD4+CD25+ T cells did not differ significantly in HPB and in the IR fraction. The presence of CD4+CD25+ T lymphocytes in the IR fraction reflects changes in the Ca2+-signaling pathway at this differentiation step of activated cells. Depending on the expression level of CD25 molecules, the population of CD4+CD25+ cells is divided in T-regulatory (CD25high) and proliferating (CD25low) subpopulations. The action of ionomycin results in a decrease in the portion of the CD4+CD25low T-cells, but it leads to an increase in the proportion of the CD4+CD25high T lymphocytes. Consequently, greater portion of CD4+CD25high T lymphocytes and smaller portion of CD4+CD25low T cells are IR cells. Expression of HLA-DR molecules can be used as the marker for the late activation step. The IR fraction is significantly rich in CD4+HLA-DR+ T lymphocytes in comparison to the blood of the same donor. The link between different differentiation steps of CD4+ T-lymphocytes and alterations in calcium ion homeostasis is discussed.
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Abbreviations
- HPB:
-
human peripheral blood
- IR:
-
ionomycin-resistant cells
- PE:
-
phycoerythrin
- HLA-DR:
-
class II human major histocompatibility complex antigen
- FITC:
-
fluorescein-isothiocyanate
- Cy5PE:
-
cyanide 5-phycoerythrin complex
- mAb:
-
monoclonal antibodies
- PBS:
-
phosphate-buffered saline
- FCS:
-
fetal calf serum
- Con A:
-
concanavalin A
- PHA:
-
Phaseolus vulgaris agglutinin
- PMA:
-
phorbol 12-myristate 13-acetate
REFERENCES
Ahmed, R., and Gray, D. (1996) Science, 272, 54–60.
Sprent, J. (1994) Cell, 76, 315–322.
Sanders, M. E., Makgoba, M. W., and Shaw, S. (1988) Immunol. Today, 9, 195–199.
Michie, C. A., McLean, A., Alcock, C., and Beverley, P. C. (1992) Nature, 360, 264–265.
Ishida, Y., and Chused, T. M. (1988) J. Exp. Med., 168, 839–852.
Miller, R. A., Flurkey, K., Molloy, M., Luby, T., and Stadecker, M. J. (1991) J. Immunol., 147, 3080–3086.
Sigova, A., Dedkova, E., Zinchenko, V., and Litvinov, I. (1999) FEBS Lett., 447, 34–38.
Haidukov, S. V., Kholodenko, I. V., and Litvinov, I. S. (2003) Tsitologiya, 45, 249–254.
Reimann, K. A., Chernoff, M., Wilkening, C. L., Nickerson, C. E., Landay, A. L., and the ACTG Immunology Advanced Technology Laboratories (2000) Clin. Diag. Lab. Immunol., 7, 352–359.
Gerosa, F., Tommasi, M., Scardoni, M., Accolla, R. S., Pozzan, T., Libonati, M., Tridente, G., and Carra, G. (1991) Mol. Immunol., 28, 159–168.
Mascher, B., Schlenke, P., and Seyfarth, M. (1999) J. Immunol. Meth., 223, 115–121.
Pala, P., Hussell, T., and Openshaw, P. J. M. (2000) J. Immunol. Meth., 243, 107–124.
Jonuleit, H., Schmitt, E., Stassen, M., Tuettenberg, A., Knop, J., and Enk, A. H. (2001) J. Exp. Med., 193, 1285–1294.
Baecher-Allan, C., Brown, J. A., Freeman, G. J., and Hafler, D. A. (2001) J. Immunol., 167, 1245–1253.
Shevach, E. M. (2002) Nat. Rev. Immunol., 2, 389–400.
Larsen, C. S., and Christiansen, N. O. (1989) Scand. J. Immunol., 30, 285–294.
Salgado, F. J., Lojo, J., Fernandez-Alonso, C. M., Vinuela, J., Cordero, O. J., and Nogueira, M. (2002) Immunol. Cell. Biol., 80, 138–147.
Johannisson, A., and Festin, R. (1995) Cytometry, 19, 343–352.
Neidhart, M., Pataki, F., Schonbachler, J., and Bruhlmann, P. (1996) Rheumatol. Int., 16, 77–87.
Raju, B., Tung, C. F., Cheng, D., Yousefzadeh, N., Condos, R., Rom, W. N., and Tse, D. B. (2001) Infect. Immun., 69, 4790–4798.
Kern, F., Docke, W. D., Reinke, P., and Volk, H. D. (1994) Int. Arch. Allergy Immunol., 104, 17–26.
Jones, D., Dang, N. H., Duvic, M., Washington, L. T., and Huh, Y. O. (2001) Am. J. Clin. Pathol., 115, 885–892.
Burnet, F. M. (1957) Australian J. Sci., 20, 67–69.
Whitney, R. B., and Sutherland, R. M. (1972) J. Cell. Physiol., 80, 329–337.
Nusslein, H. G., Frosch, K. H., Woith, W., Lane, P., Kalden, J. R., and Manger, B. (1996) Eur. J. Immunol., 26, 846–850.
Gelfand, E. W., Cheung, R. K., Mills, G. B., and Grinstein, S. (1988) Eur. J. Immunol., 18, 917–922.
Song, S., Goodwin, J., Zhang, J., Babbitt, B., and Lathey, J. L. (2002) Clin. Diagn. Lab. Immunol., 9, 708–712.
Kaiser, N., and Edelman, I. S. (1978) Cancer Res., 38, 3599–3603.
Kay, J. E. (1971) Exp. Cell. Res., 68, 11–16.
Bard, E., Colwill, R., L’Anglais, R., and Kaplan, J. G. (1978) Can. J. Biochem., 56, 900–904.
Greene, W. C., Parker, C. M., and Parker, C. W. (1976) Cell. Immunol., 25, 74–89.
Vandenberghe, P., Verwilghen, J., van Vaeck, F., and Ceuppens, J. L. (1993) Immunology, 78, 210–217.
Komada, H., Nakabayashi, H., Hara, M., and Izutsu, K. (1996) Cell. Immunol., 173, 215–220.
Mills, G. B., Cheung, R. K., Grinstein, S., and Gelfand, E. W. (1985) J. Immunol., 134, 1640–1643.
Larsen, C. S., Christiansen, N. O., and Esmann, V. (1988) Scand. J. Immunol., 28, 167–175.
Kanner, S. B., Grosmaire, L. S., Blake, J., Schieven, G. L., Masewicz, S., Odum, N., and Ledbetter, J. A. (1995) Tissue Antigens, 46, 145–54.
Mackay, C. R. (1991) Immunol. Today, 12, 189–192.
McCloskey, T. W., Bakshi, S., Than, S., Arman, P., and Pahwa, S. (1999) Blood, 94, 1829–1833.
Sallusto, F., Mackay, C. R., and Lanzavecchia, A. (2000) Annu. Rev. Immunol., 18, 593–620.
Haidukov, S. V., and Litvinov, I. S. (2003) Biol. Membr. (Moscow), 20, 333–340.
Miller, R. A., Philosophe, B., Ginis, I., Weil, G., and Jacobson, B. (1989) J. Cell. Physiol., 138, 175–182.
Grossmann, A., Ledbetter, J. A., and Rabinovitch, P. S. (1990) J. Gerontol., 45, 81–86.
Bocharova, I. V., Mezhlutova, M. B., Haidukov, S. V., Litvinov, I. S., Yakhin, R. T., and Nikonenko, B. V. (1997) Byul. Eksp. Biol. Med., 123, 553–555.
Nikonenko, B. V., Haidukov, S. V., Litvinov, I. S., Bocharova, I. V., and Apt, A. S. (2001) Byul. Eksp. Biol. Med., 131, 548–549.
Agafonova, S. A., Khaidukov, S. V., Kotel’nikova, O. V., and Litvinov, I. S. (1997) Abst. Fourth Int. Symp. Clinical Immunology, Amsterdam, 19–22 June, p. 55.
Schwinzer, R., and Siefken, R. (1996) Transpl. Immunol., 4, 61–63.
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Translated from Biokhimiya, Vol. 70, No. 6, 2005, pp. 838–849.
Original Russian Text Copyright © 2005 by Khaidukov, Litvinov.
Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM04-154, October 24, 2004.
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Khaidukov, S.V., Litvinov, I.S. Calcium Homeostasis Change in CD4+ T Lymphocytes from Human Peripheral Blood during Differentiation in vivo . Biochemistry (Moscow) 70, 692–702 (2005). https://doi.org/10.1007/s10541-005-0170-8
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DOI: https://doi.org/10.1007/s10541-005-0170-8