Skip to main content
SpringerLink
Log in

Cloning and expression of mistletoe lectin III B-subunit

  • Published:
Biochemistry (Moscow) Aims and scope Submit manuscript

Abstract

Aqueous extracts of mistletoe (Viscum album L.) contain toxic proteins (lectins) MLI (viscumin), MLII, and MLIII. We previously cloned the gene encoding MLIII precursor. In the present study, a gene fragment encoding the carbohydrate-binding subunit of mistletoe toxic lectin MLIII was cloned and expressed in Escherichia coli cells. The structure and immunochemical properties of recombinant MLIII B-subunit were investigated using a panel of monoclonal antibodies against ML-toxins. Sugar-binding activity of recombinant MLIII B-subunit was determined by ELISA. Amino acid sequence analysis of the cloned MLIII compared with known mistletoe toxins and other ribosome inactivating type II proteins (ricin, abrin a, and nigrin b B-subunits) revealed essential features of the recombinant MLIIIB primary structure that could determine sugar specificity of the lectin as well as immunomodulating and anti-tumor properties of mistletoe extracts.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

ABB:

abrin a subunit B

GalNAc:

N-acetylgalactosamine

Gnd-HCl:

guanidine hydrochloride

IPTG:

isopropyl-β-D-thiogalactoside

MLI, MLII, and MLIII:

European mistletoe (Viscum album) lectins I (viscumin), II, and III

NK:

natural killers

MLIB and MLIIIB:

native B-subunits of MLI and MLIII

mAB:

monoclonal antibody

RIPI and RIPII:

ribosome-inactivating protein type I and II

rMLIA and rMLIB:

recombinant MLI subunits A and B

rMLIIIA and rMLIIIB:

recombinant MLIII subunits A and B

RTB:

ricin subunit B

SNAV:

nigrin b (Sambucus nigra agglutinin V)

SNAVB:

nigrin b subunit B

KML-1, KML-2, and KML-3:

lectins 1, 2, and 3 from Korean mistletoe Viscum album subsp. coloratum

KMLB1, KMLB2, and KMLB3:

B-subunits of KML-1, KML-2, and KML-3

VCA:

lectin from Korean mistletoe Viscum album subsp. coloratum (Viscum album subsp. coloratum agglutinin)

VCAB:

VCA subunit B

DTT:

dithiothreitol

REFERENCES

  1. Hajto, T., Hostanska, K., and Gabius, H.-J. (1989) Cancer Res., 49, 4803–4808.

    Google Scholar 

  2. Buessing, A., Suzart, K., Bergmann, J., Pfuller, U., Schietzel, M., and Schweizer, K. (1996) Cancer Lett., 99, 59–72.

    Google Scholar 

  3. Eifler, R., Pfueller, K., Goeckeritz, W., and Pfueller, U. (1993) in Lectins: Biology, Biochemistry, Clinical Biochemistry (Basu, J., Kundu, M., and Chakrabarti, P., eds.) Vol. 9, Wiley Eastern Ltd., India, pp. 144–151.

    Google Scholar 

  4. Barbieri, L., Battelli, M. G., and Stirpe, F. (1993) Biochim. Biophys. Acta, 1154, 237–282.

    Google Scholar 

  5. Endo, Y., Tsurugi, K., and Franz, H. (1988) FEBS Lett., 2, 378–380.

    Google Scholar 

  6. Franz, H., Ziska, P., and Kindt, A. (1981) Biochem. J., 195, 481–484.

    Google Scholar 

  7. Stirpe, F., Barbieri, L., Batelli, M. G., Soria, M., and Lappi, D. A. (1992) Biotechnology, 10, 405–424.

    Google Scholar 

  8. Dietrich, J. B., Ribereau-Gayon, G., Jung, M. L., Franz, H., Beck, J. P., and Anton, R. (1992) Anti-Cancer Drugs, 3, 507–511.

    Google Scholar 

  9. Tonevitsky, A. G., Agapov, I. I., Pevzner, I. B., Maluchenko, N. V., Moisenovich, M. M., Pfueller, U., and Kirpichnikov, M. P. (2004) Biochemistry (Moscow), 69, 642–650.

    Google Scholar 

  10. Ziska, P., Gelbin, M., and Franz, H. (1993) in Lectins: Biology, Biochemistry, Clinical Biochemistry (Van Driessche, E., Franz, H., and Beeckmans, S., eds.) Vol. 8, Textop, Hellerup, Denmark, pp. 10–13.

    Google Scholar 

  11. Wu, A. M., Chin, L.-K., Franz, H., Pfuller, U., and Herp, A. (1992) Biochim. Biophys. Acta, 1117, 232–234.

    Google Scholar 

  12. Kopp, J., Korner, I.-J., Pfueller, U., Gockeritz, W., Eifler, R., Pfuller, K., and Franz, H. (1993) in Lectins: Biology, Biochemistry, Clinical Biochemistry (Van Driessche, E., Franz, H., and Beeckmans, S., eds.) Vol. 8, Textop, Hellerup, Denmark, pp. 41–47.

    Google Scholar 

  13. Eck, J., Langer, M., Moeckel, B., Witthohn, K., Zinke, H., and Lentzen, H. (1999) Eur. J. Biochem., 265, 788–797.

    Google Scholar 

  14. Saitou, N., and Nei, S. (1987) Mol. Biol. Evol., 4, 406–425.

    Google Scholar 

  15. Agapov, I. I., Tonevitsky, A. G., Maluchenko, N. V., Moisenovich, M. M., Bulah, Y. A., and Kirpichnikov, M. P. (1999) FEBS Lett., 464, 63–66.

    Google Scholar 

  16. Moisenovich, M. M., Egorova, S. G., Chelnokova, O. V., Demina, I. A., Polosukhina, E. R., Kozlovskaya, N. V., Popova, E. N., Fattakhova, G. V., Solopova, O. N., and Agapov, I. I. (2000) Russ. J. Immunol., 5, 376–384.

    Google Scholar 

  17. Tonevitsky, A. G., Agapov, I., Temiakov, D., Moysenovich, M. M., Maluchenko, N. V., Solopova, O. N., Wü rzner, G., and Pfueller, U. (1999) Arzneim.-Forsch./Drug Res., 49, 970–975.

    Google Scholar 

  18. Fattakhova, G. V., Agapov, I. I., Solopova, O. N., Moisenovich, M. M., and Tonevitsky, A. G. (2001) Biotekhnologiya, 3, 59–70.

    Google Scholar 

  19. Rutenber, E., and Robertus, J. D. (1991) Proteins, 10, 260–269.

    Google Scholar 

  20. Hatakeyama, T., Yamasaki, N., and Funatsu, G. (1986) J. Biochem., 100, 781–788.

    Google Scholar 

  21. Wu, A. M., Wu, J. H., Herp, A., Chow, L.-P., and Lin, J.-Y. (2001) Life Sci., 69, 2027–2038.

    Google Scholar 

  22. Van Damme, E. J. M., Barre, A., Rouge, P., van Leuven, F., and Peumans, W. J. (1996) Eur. J. Biochem., 237, 505–513.

    Google Scholar 

  23. Niwa, H., Tonevitsky, A. G., Agapov, I. I., Saward, S., Pfüller, U., and Palmer, R. A. (2003) Eur. J. Biochem., 270, 2739–2749.

    Google Scholar 

  24. Zimmermann, R., and Pfueller, U. (1998) in COST 98 “Effect of Antinutrients on the Nutritional Value of Legume Diets” (Bardocz, S., Pfueller, U., and Pustzai, A., eds.) Vol. 5, European Communities, Brussels, pp. 55–62.

    Google Scholar 

  25. Tonevitsky, A. G., Rakhmanova, V. A., Agapov, I. I., Shamshiev, A. T., Usacheva, E. A., Prokof’ev, S. A., Denisenko, O. N., Alekseev, Yu. O., and Pfueller, U. (1995) Immunol. Lett., 44, 31–34.

    Google Scholar 

  26. Tonevitsky, A. G., Agapov, I. I., Pevzner, I. B., Maluchenko, N. V., Moisenovich, M. M., Yurkova, M., and Pfueller, U. (2004) Arzneim.-Forsch./Drug Res., 54, 242–249.

    Google Scholar 

  27. Soler, M. H., Stoeva, S., Schwamborn, C., Wilhelm, S., Stiefel, T., and Voelter, W. (1996) FEBS Lett., 399, 153–157.

    Google Scholar 

  28. Park, C. H., Lee, D. W., Kang, T. B., Lee, K. H., Yoon, T. J., Kim, J. B., Do, M. S., and Song, S. K. (2001) Mol. Cells, 12, 215–220.

    Google Scholar 

  29. Pfueller, U. (1996) in Proc. II Scientific Workshop of COST 98, Budapest (Bardocz, S., Gelencser, E., and Pusztai, A., eds.) Vol. 11, Office for Official Publications of the European Communities, Luxemburg, p. 46.

    Google Scholar 

  30. Tonevitsky, A. G., Marx, U., Agapov, I., and Moisenovich, M. (2002) Arzneim.-Forsch./Drug Res., 52, 67–71.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Faculty of Biology, Lomonosov Moscow State University, 119899, Moscow, Russia

    I. B. Pevzner, I. I. Agapov, N. V. Maluchenko, M. M. Moisenovich, A. G. Tonevitsky & M. P. Kirpichnikov

  2. Institute of Phytochemistry, University of Witten/Herdecke, Stockumer Str. 10, D-58448, Witten, Germany

    U. Pfueller & K. Pfueller

Authors
  1. I. B. Pevzner
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. I. Agapov
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. U. Pfueller
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. K. Pfueller
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. N. V. Maluchenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M. M. Moisenovich
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. A. G. Tonevitsky
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. M. P. Kirpichnikov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. G. Tonevitsky.

Additional information

Translated from Biokhimiya, Vol. 70, No. 3, 2005, pp. 378–389.

Original Russian Text Copyright © 2005 by Pevzner, Agapov, Pfueller, Pfueller, Maluchenko, Moisenovich, Tonevitsky, Kirpichnikov.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pevzner, I.B., Agapov, I.I., Pfueller, U. et al. Cloning and expression of mistletoe lectin III B-subunit. Biochemistry (Moscow) 70, 306–315 (2005). https://doi.org/10.1007/s10541-005-0116-1

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10541-005-0116-1

Key words

Search

Navigation