Abstract
Eighteen clones representing copies of four Kunitz-type proteinase inhibitor group B genes (PKPI-B) obtained by polymerase chain reaction cloning of potato (Solanum tuberosum L. cv. Istrinskii) genomic DNA were sequenced and analyzed. Three new genes were found and named PKPI-B1, PKPI-B2, and PKPI-B10: these were represented by five, two, and seven clones, respectively. The remaining four clones corresponded to the formerly characterized PKPI-B9 gene. These data show that at least four PKPI-B encoding genes are harbored in the genome of potato cv. Istrinskii. Their analysis suggests that variability of PKPI-B encoding genes in potato is limited and could be explained by cross-hybridization events in the ancestor forms rather than by random mutagenesis.
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REFERENCES
Mosolov, V. V., and Valueva, T. A. (1993) Plant Protein Inhibitors of Proteolytic Enzymes [in Russian], VINITI, Moscow.
Ishikawa, A., Ohta, S., Matsuoka, K., Hattori, T., and Nakamura, K. (1994) Plant Cell Physiol., 35, 303–312.
Revina, T. A., Speranskaya, A. S., Kladnitskaya, G. V., Shevelev, A. B., and Valueva, T. A. (2004) Biochemistry (Moscow), 69, 1092–1098.
Poussereau, N., Creton, S., Billon-Grand, G., Rascl, C., and Fevre, M. (2001) Microbiology, 147, 717–726.
Valueva, T. A., Revina, T. A., Kladnitskaya, G. V., and Mosolov, V. V. (1998) FEBS Lett., 426, 131–134.
Valueva, T. A., Revina, T. A., Gvozdeva, E. L., Gerasimova, N. G., and Ozeretskovskaya, O. L. (2003) Bioorg Khim., 29, 499–504.
Heibges, A., Glaczinski, H., Ballvora, A., Salamini, F., and Gebhardt, C. (2003) Mol. Gen. Genom., 269, 526–534.
Heibges, A., Glaczinski, H., Ballvora, A., Salamini, F., and Gebhardt, C. (2003) Mol. Gen. Genom., 269, 535–541.
Banfalvi, Z., Molnar, A., Molnar, G., Lakatos, L., and Szabo, L. (1996) FEBS Lett., 383, 159–164.
Pouvreau, L., Gruppen, H., van Koningsveld, G. A., van den Broek, L. A. M., and Voragen, A. G. J. (2003) J. Agric. Food Chem., 51, 5001–5003.
Maganja, D. B., Strukelj, B., Pungercar, J., Gubensek, F., Turk, V., and Kregar, I. (1992) Plant Mol. Biol., 20, 311–313.
Speranskaya, A. S., Dorokhin, A. V., Novikova, S. I., Shevelev, A. B., and Valueva, T. A. (2003) Genetika, 39, 1490–1497.
Valueva, T. A., Revina, T. A., Mosolov, V. V., and Mentele, R. (2000) Biol. Chem., 381, 1215–1221.
Barrett, A. J., Rawlings, N. D., and O’Brien, E. A. (2001) J. Struct. Biol., 134, 95–102.
Mosolov, V. V., and Shul’gin, M. N. (1986) Planta, 167, 595–600.
Garcia-Olmedo, F., Salcedo, G., Sanchez-Monge, R., Gomez, L., Royo, J., and Carbonero, P. (1987) Oxford Surv. Plant Mol. Cell Biol., 4, 275–334.
Ezhova, T. A., Soldatova, O. P., Penin, A. A., and Shestakov, S. V. (2002) The Plant Genome Molecular-Genetic Mapping [in Russian], MAKS Press, Moscow.
Sambrook, J., Fritch, E., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd Edn., Vol. 3, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
Felsenstein, J. (1988) Ann. Rev. Genet., 22, 521–565.
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Translated from Biokhimiya, Vol. 70, No. 3, 2005, pp. 360–369.
Original Russian Text Copyright © 2005 by Speranskaya, Krinitsina, Poltronieri, Fasano, Santino, Shevelev, Valueva.
Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM04-234, February 6, 2005.
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Speranskaya, A.S., Krinitsina, A.A., Poltronieri, P. et al. Molecular cloning of Kunitz-type proteinase inhibitor group B genes from potato. Biochemistry (Moscow) 70, 292–299 (2005). https://doi.org/10.1007/s10541-005-0114-3
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DOI: https://doi.org/10.1007/s10541-005-0114-3