Abstract
The mechanism of the increased accumulation (overproduction) of citric acids in the yeast Yarrowia lipolytica while growing in the presence of glucose under nitrogen deficiency was investigated. The limitation of the yeast growth by the source of nitrogen decreases the total content of nucleotides and increases the ratios of ATP/AMP and NADH/NAD+. NAD+-Dependent isocitrate dehydrogenase, an enzyme of the tricarboxylic acid cycle playing a key role in the regulation of biosynthesis of citric and isocitric acids, was isolated from Y. lipolytica. The molecular weights of the native enzyme and its subunits were found to be 412 and 52 kD, respectively. It is concluded that the enzyme is a homooligomer consisting of eight subunits. Investigation of the effect of some intermediates of the tricarboxylic acid cycle on the activity of this enzyme suggests that the enhanced excretion of citric acids can be caused by the inhibition of NAD+-dependent isocitrate dehydrogenase due to the decrease in the content of AMP and increase in the NADH/NAD+ ratio in the cells of Y. lipolytica under depletion of nitrogen.
Similar content being viewed by others
REFERENCES
C. Ratledge (1978) NoChapterTitle A. H. Rose (Eds) Economic Microbiology Academic Press N.-Y.-London 263–301
T. V. Finogenova A. V. Grinchak V. I. Illarionova N. V. Shishkanova (1979) Prikl. Biokhim. Mikrobiol. 15 811–816 Occurrence Handle538009 Occurrence Handle1:CAS:528:DyaL3cXhsl2jt7k%3D
T. V. Finogenova N. V. Shishkanova I. A. Kataeva (1989) Mikrobiologiya 58 387–392 Occurrence Handle1:CAS:528:DyaL1MXltlSntr4%3D
T. V. Finogenova (1982) Doctoral dissertation IBFM Pushchino
Finogenova, T. V. (1991) in Metabolism of Alkanes and Oversynthesis of Products by Microorganisms [in Russian], Pushchino, pp. 96–114.
P. R. Burkholder (1943) Amer. J. Botany 30 206 Occurrence Handle1:CAS:528:DyaH3sXitlegsg%3D%3D
J. W. T. Wimpenny A. J. Firth (1972) J. Bacteriol. 111 24–32 Occurrence Handle4360220 Occurrence Handle1:CAS:528:DyaE38XksFymt7c%3D
G. A. Kimmich J. Randless J. S. Brand (1975) Analyt. Biochem. 69 187–206 Occurrence Handle2029 Occurrence Handle1:CAS:528:DyaE28XktVCguw%3D%3D
C. Bernofsky M. Swan (1973) Analyt. Biochem. 53 452–458 Occurrence Handle4351948 Occurrence Handle1:CAS:528:DyaE3sXktVansro%3D
P. A. Srere (1969) Meth. Enzymol. 13 3–11 Occurrence Handle10.1016/0076-6879(69)13005-0 Occurrence Handle1:CAS:528:DyaE3cXks1aktrk%3D
C. B. Anfinsen (1955) Meth. Enzymol. 1 695–698 Occurrence Handle1:CAS:528:DC%2BD1cXhtFWjsro%3D
A. Kornberg W. E. J. Pricer (1951) J. Biol. Chem. 189 123–126 Occurrence Handle14832224 Occurrence Handle1:CAS:528:DyaG3MXivFyrsA%3D%3D
G. H. Dixon H. L. Kornberg (1959) Biochem. J. 72 3
A. K. Romanova (1980) Biochemical Methods of Study of Autotrophism in Microorganisms Nauka Moscow
M. M. Bradford (1976) Analyt. Biochem. 72 248–254 Occurrence Handle942051 Occurrence Handle1:CAS:528:DyaE28XksVehtrY%3D
P. A. Botham C. J. Ratledge (1979) Gen. Microbiol. 114 361–375 Occurrence Handle1:CAS:528:DyaL3cXhtVOjsbo%3D
L. Webb (1966) Inhibitors of Enzymes and Metabolism. General Principles of Inhibition Mir Moscow
C. L. Slayman (1973) J. Bacteriol. 114 752–766 Occurrence Handle4267534 Occurrence Handle1:CAS:528:DyaE3sXhsFKgsbk%3D
D. E. Atkinson (1977) Cellular Energy Metabolism and Its Regulation Academic Press N. Y.-San Francisco-London
C. J. Knowles (1977) Symp. Soc. Gen. Microbiol. 27 241–283 Occurrence Handle1:CAS:528:DyaE1MXhvVOqug%3D%3D
L. D. Barnes J. J. McGuire D. E. Atkinson (1972) Biochemistry 11 4322–4329 Occurrence Handle4342903 Occurrence Handle1:CAS:528:DyaE3sXhsVGr
C. T. Evans C. Ratledge (1985) Can. J. Microbiol. 31 845–850 Occurrence Handle1:CAS:528:DyaL2MXmtV2lsrg%3D Occurrence Handle10.1139/m85-157
D. M. Sokolov N. Yu. Solodovnikova A. A. Sharyshev T. B. Finogenova (1995) Prikl. Biokhim. Mikrobiol. 31 528–533 Occurrence Handle1:CAS:528:DyaK2MXptlSqu7g%3D
J. A. Hathaway D. E. Atkinson (1963) J. Biol. Chem. 238 2875–2881 Occurrence Handle14063317 Occurrence Handle1:CAS:528:DyaF3sXkt1aqs7s%3D
D. E. Atkinson J. A. Hathaway (1965) J. Biol. Chem. 240 2682–2690 Occurrence Handle14304886 Occurrence Handle1:CAS:528:DyaF2MXktVCmurw%3D
J. L. Gabriel C. W. E. Plaut (1991) Biochemistry 30 2594–2599 Occurrence Handle2001349 Occurrence Handle1:CAS:528:DyaK3MXhtFOlsL4%3D
J. L. Gabriel C. W. E. Plaut (1990) Biochemistry 29 3528–3533 Occurrence Handle2162196 Occurrence Handle1:CAS:528:DyaK3cXhvFCns7w%3D
A.-P. Lin M. T. McCammon L. McAlister-Henn (2001) Biochemistry 40 14291–14301 Occurrence Handle11714283 Occurrence Handle1:CAS:528:DC%2BD3MXnvVGmsLk%3D
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Biokhimiya, Vol. 69, No. 12, 2004, pp. 1706–1714.
Original Russian Text Copyright © 2004 by Morgunov, Solodovnikova, Sharyshev, Kamzolova, Finogenova.
Rights and permissions
About this article
Cite this article
Morgunov, I.G., Solodovnikova, N.Y., Sharyshev, A.A. et al. Regulation of NAD+-dependent isocitrate dehydrogenase in the citrate producing yeast Yarrowia lipolytica . Biochemistry (Moscow) 69, 1391–1398 (2004). https://doi.org/10.1007/s10541-005-0086-3
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/s10541-005-0086-3