Skip to main content
Log in

Chemical Genetics: Drug Screens in Zebrafish

  • Published:
Bioscience Reports

High throughput chemical genetic screens for compounds with specific biological activity in a whole organism are feasible using zebrafish embryos. At least two medium to large scale drug screens have been carried out to date, leading to the identification of compounds that disturb zebrafish development. Chemical genetics using zebrafish embryos may become an important step in the discovery of drugs and their targets.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • T. R. Brummelkamp R. Bernards (2003) ArticleTitleNew tools for functional mammalian cancer genetics Nat. Rev. Cancer 3 781–789 Occurrence Handle10.1038/nrc1191 Occurrence Handle1:CAS:528:DC%2BD3sXotFChtLc%3D

    Article  CAS  Google Scholar 

  • J. Chan P. E. Bayliss J. M. Wood T. M. Roberts (2002) ArticleTitleDissection of angiogenic signaling in zebrafish using a chemical genetic approach Cancer Cell 1 257–267 Occurrence Handle1:CAS:528:DC%2BD38XjsFGlt74%3D

    CAS  Google Scholar 

  • A. Dodd P. M. Curtis L. C. Williams D. R. Love (2000) ArticleTitleZebrafish: bridging the gap between development and disease Hum. Mol. Genet. 16 2443–2449

    Google Scholar 

  • A. Dodd S. P. Chambers D. R. Love (2004) ArticleTitleShort interfering RNA-mediated gene targeting in the zebrafish FEBS Lett. 561 89–93 Occurrence Handle10.1016/S0014-5793(04)00129-2 Occurrence Handle1:CAS:528:DC%2BD2cXhvFansrc%3D

    Article  CAS  Google Scholar 

  • B. W. Draper P. A. Morcos C. B. Kimmel (2001) ArticleTitleInhibition of zebrafish fgf8 pre-mRNA slpicing with morpholino oligos: a quantifiable method for gene knockdown Genesis 30 154–156 Occurrence Handle10.1002/gene.1053 Occurrence Handle1:CAS:528:DC%2BD3MXmsVOlsrY%3D

    Article  CAS  Google Scholar 

  • W. Driever L. Solnica-Krezel A. F. Schier S. C. Neuhauss J. Malicki D. L. Stemple D. Y. Stainier F. Zwartkruis S. Abdelilah Z. Rangini J. Belak C. Boggs (1996) ArticleTitleA genetic screen for mutations affecting embryogenesis in zebrafish Development 123 37–46 Occurrence Handle1:CAS:528:DyaK2sXhtVOmt7c%3D

    CAS  Google Scholar 

  • Genesis (2001) ArticleTitleMorpholino gene knock down issue Genesis 30 89–200

    Google Scholar 

  • G. Golling A. Amsterdam Z. Sun M. Antonelli E. Maldonado W. Chen S. Burgess M. Haldi K. Artzt S. Farrington S. Y. Lin R. M. Nissen N. Hopkins (2002) ArticleTitleInsertional mutagenesis in zebrafish rapidly identifies genes essential for early vertebrate development Nat. Genet. 31 135–140 Occurrence Handle10.1038/ng896 Occurrence Handle1:CAS:528:DC%2BD38XktVKgtrY%3D

    Article  CAS  Google Scholar 

  • Habeck, H., Odenthal, J., Walderich, B., Maischein, H., Schulte-Merker S. and the Tuebingen 2000 screen consortium (2002) Analysis of a zebrafish VEGF receptor mutant reveals specific disruption of angiogenesis. Curr. Biol. 12:1405–1412

    Google Scholar 

  • P. Haffter M. Granato M. Brand M. C. Mullins M. Hammerschmidt D. A. Kane J. Odenthal F. J. Eeden Particlevan Y. J. Jiang C. P. Heisenberg R. N. Kelsh M. Furutani-Seiki E. Vogelsang D. Beuchle U. Schach C. Fabian C. Nusslein-Volhard (1996) ArticleTitleThe identification of genes with unique and essential functions in the development of the zebrafish, Danio rerio Development 123 1–36 Occurrence Handle1:STN:280:ByiC2MbntFQ%3D

    CAS  Google Scholar 

  • G. J. Hannon (2002) ArticleTitleRNA interference Nature 418 244–251 Occurrence Handle10.1038/418244a Occurrence Handle1:CAS:528:DC%2BD38XltFGmu7Y%3D

    Article  CAS  Google Scholar 

  • A. F. Hurlstone A. P. Haramis E. Wienholds H. Begthel J. Korving F. Eeden ParticleVan E. Cuppen D. Zivkovic R. H. Plasterk H. Clevers (2003) ArticleTitleThe Wnt/beta-catenin pathway regulates cardiac valve formation Nature 425 633–637 Occurrence Handle10.1038/nature02028 Occurrence Handle1:CAS:528:DC%2BD3sXnvV2hsL8%3D

    Article  CAS  Google Scholar 

  • S. M. Khersonsky D. W. Jung T. W. Kang D. P. Walsh H. S. Moon H. Jo E. M. Jacobson V. Shetty T. A. Neubert Y. T. Chang (2003) ArticleTitleFacilitated forward chemical genetics using a tagged triazine library and zebrafish embryo screening J. Am. Chem. Soc. 125 11804–11805 Occurrence Handle10.1021/ja035334d Occurrence Handle1:CAS:528:DC%2BD3sXnt1OgsrY%3D

    Article  CAS  Google Scholar 

  • R. S. Lokey (2003) ArticleTitleForward chemical genetics: progress and obstacles on the path to a new pharmacopoeia Curr. Opin. Chem. Biol. 7 91–96 Occurrence Handle10.1016/S1367-5931(02)00002-9 Occurrence Handle1:CAS:528:DC%2BD3sXltlejsw%3D%3D

    Article  CAS  Google Scholar 

  • A. Nasevicius S. C. Ekker (2000) ArticleTitleEffective targeted gene ‘knockdown’ in zebrafish Nat. Genet. 26 216–220 Occurrence Handle1:CAS:528:DC%2BD3cXntlGmsL8%3D

    CAS  Google Scholar 

  • A. C. Oates A. E. Bruce R. K. Ho (2000) ArticleTitleToo much interference: injection of double-stranded RNA has nonspecific effects in the zebrafish embryo Dev. Biol. 224 20–28 Occurrence Handle10.1006/dbio.2000.9761 Occurrence Handle1:CAS:528:DC%2BD3cXkvVWqtLw%3D

    Article  CAS  Google Scholar 

  • R. T. Peterson B. A. Link J. E. Dowling S. L. Schreiber (2000) ArticleTitleSmall molecule developmental screens reveal the logic and timing of vertebrate development Proc. Natl. Acad. Sci. USA 97 12965–12969 Occurrence Handle1:CAS:528:DC%2BD3cXosVKqtrk%3D

    CAS  Google Scholar 

  • R. T. Peterson J. D. Mably J. N. Chen M. C. Fishman (2001) ArticleTitleConvergence of distinct pathways to heart patterning revealed by the small molecule concentramide and the mutation heart-and-soul Curr. Biol. 11 1481–1491 Occurrence Handle10.1016/S0960-9822(01)00482-1 Occurrence Handle1:CAS:528:DC%2BD3MXnsF2msrs%3D

    Article  CAS  Google Scholar 

  • A. Sar ParticleVan der D. Zivkovic J. Hertog Particleden (2002) ArticleTitleEye defects in receptor protein-tyrosine phosphatase alpha knock-down zebrafish Dev. Dyn. 223 292–297

    Google Scholar 

  • E. Wienholds S. Schulte-Merker B. Walderich R. H. A. Plasterk (2002) ArticleTitleTarget-selected inactivation of the zebrafish rag-1 gene Science 297 99–102 Occurrence Handle10.1126/science.1071762 Occurrence Handle1:CAS:528:DC%2BD38XltFGrurk%3D

    Article  CAS  Google Scholar 

  • E. Wienholds M. J. Koudijs F. J. Eeden Particlevan E. Cuppen R. H. Plasterk (2003) ArticleTitleThe microRNA-producing enzyme Dicer1 is essential for zebrafish development Nat. Genet. 35 217–218 Occurrence Handle10.1038/ng1251 Occurrence Handle1:CAS:528:DC%2BD3sXosFWrtr0%3D

    Article  CAS  Google Scholar 

  • L. I. Zon (1999) ArticleTitleZebrafish: a new model for human disease Genome Res. 9 99–100 Occurrence Handle1:CAS:528:DyaK1MXhsFyjsb8%3D

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Jeroen den Hertog.

Rights and permissions

Reprints and permissions

About this article

Cite this article

den Hertog, J. Chemical Genetics: Drug Screens in Zebrafish. Biosci Rep 25, 289–297 (2005). https://doi.org/10.1007/s10540-005-2891-8

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10540-005-2891-8

Keywords

Navigation