Two established cancer cell lines, MCF-7 and Ishikawa, were both obtained directly from a cell repository and through another laboratory. The karyotypes from the two MCF-7 cell lines had up to 83 chromosomes and similarities for chromosomal gain and structural abnormalities. The two Ishikawa cell lines had up to 60 chromosomes with only a missing X as the common chromosome abnormality. CGH studies were performed by co-hybridizing the two Ishikawa or MCF-7 cell lines to normal metaphases. The differences seen between the two MCF-7 cell cultures reflect changes due to passage number and culture conditions. For Ishikawa, DNA polymorphic data and mutation studies suggest that the two cell lines are not derived from the same established tumor cell line. Our study shows the utilization of CGH in comparing cell lines originating from the same specimen. Our study also demonstrates the necessity for periodically evaluating cell lines to confirm their origin.
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J. M. Lapierre V. Cacheux F. Da Silva N. Collot N. Hervy J. Wiss G. Tachdjian (1998) Ann. Genet. 41 56–62 Occurrence Handle9599653
A. Kallioniemi O. Kallioniemi D. Sudar D. Rutovitz J. W. Gray F. Waldman D. Pinkel (1992) Science 258 818–821 Occurrence Handle1359641
N. Wang (2002) Am. J. Med. Genet. 115 118–124 Occurrence Handle10.1002/ajmg.10687 Occurrence Handle12407691
N. Joshi S. G. Grant (2002) Am. J. Hum. Genet. 71 IssueIDsuppl 251
S. R. Wolman G. H. Heppner (1992) J. Natl. Cancer Inst. 84 469–470 Occurrence Handle1545431
A. Fabarius R. Hehlmann P. H. Duesberg (2003) Cancer Genet. Cytogenet. 143 59–72 Occurrence Handle10.1016/S0165-4608(03)00003-7 Occurrence Handle12742157
C. Jones J. Payne D. Wells J. D. A. Delhanty S. R. Lakhani A. Kortenkamp (2000) Cancer Genet. Cytogenet. 117 153–158 Occurrence Handle10.1016/S0165-4608(99)00158-2 Occurrence Handle10704689
J. Whang-Peng E. C. Lee C.-S. Kao-Shan K. Seibert M. Lippman (1983) J. Natl. Cancer Inst. 71 687–695 Occurrence Handle6578363
M. Gold (1986) A Conspiracy of Cells: One Woman’s Immortal Legacy and the Medical Scandal it Caused State University of New York Press Albany
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Wenger, S.L., Senft, J.R., Sargent, L.M. et al. Comparison of Established Cell Lines at Different Passages by Karyotype and Comparative Genomic Hybridization. Biosci Rep 24, 631–639 (2004). https://doi.org/10.1007/s10540-005-2797-5
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DOI: https://doi.org/10.1007/s10540-005-2797-5