Skip to main content

Advertisement

SpringerLink
Log in

Pre-UV-Treatment of Cells Results in Enhanced Host Cell Reactivation of a UV Damaged Reporter Gene in CHO-AA8 Chinese Hamster Ovary Cells but Not in Transcription-Coupled Repair Deficient CHO-UV61 Cells

  • Published:
Bioscience Reports

We have used a non-replicating recombinant adenovirus, Ad5MCMVlacZ, which expresses the β-galactosidase reporter gene, to examine both constitutive and inducible repair of UV-damaged DNA in repair proficient CHO-AA8 Chinese hamster ovary cells and in mutant CHO-UV61 cells which are deficient in the transcription-coupled repair (TCR) pathway of nucleotide excision repair. Host cell reactivation (HCR) of β-galactosidase activity for UV-irradiated Ad5MCMVlacZ was significantly reduced in non-irradiated CHO-UV61 cells compared to that in non-irradiated CHO-AA8 cells suggesting that repair in the transcribed strand of the UV-damaged reporter gene in untreated cells utilizes TCR. Prior UV-irradiation of cells with low UV fluences resulted in a transient enhancement of HCR for expression of the UV-damaged reporter gene in CHO-AA8 cells but not in TCR deficient CHO-UV61 cells. These results suggest the presence of an inducible DNA pathway in CHO cells that results from an enhancement of TCR or a mechanism that involves the TCR pathway.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Rainbow, A. J. (1981) Reactivation of viruses. In: Short Term Tests for Chemical Carcinogens, (H. F. Stich and R. H. C. San eds.), Springer-Verlag, New York. pp. 20–35

  2. M. J. Defais P. C. Hanawalt A. Sarasin (1983) ArticleTitleViral probes for DNA repair Adv. Radiat. Biol. 10 1–37

    Google Scholar 

  3. C. B. Bennett A. J. Rainbow (1988) ArticleTitleEnhanced reactivation and mutagenesis of UV-irradiated adenovirus in normal human fibroblasts Mutagenesis 3 157–164 Occurrence Handle3288839

    PubMed  Google Scholar 

  4. W. P. Jeeves A. J. Rainbow (1983) ArticleTitleUV enhanced reactivation of U.V. and gamma- irradiated adenovirus in Cockayne syndrome and Xeroderma pigmentosum fibroblasts Int. J. Radiat. Biol. 43 625–647

    Google Scholar 

  5. A. J. Rainbow B. C. McKay M. A. Francis (2000) ArticleTitleRecombinant adenoviruses as expression vectors and as probes for DNA repair in human cells Gene Ther. Mol. Biol. 5 87–101

    Google Scholar 

  6. Rainbow, A. J., Pitsikas, P., Caney, C., Boszko, I., McKay, B. C., and Francis, M. A. (2005) Reactivation of UV-damaged viruses and reporter genes in mammalian cells. In: From DNA Photolesions to Mutations, Skin Cancer and Cell Death (Regen Drouin, Evelene Sage and Mahmoud Roubhia eds.) Comprehensive Series in Photosciences (Donat-P. Hader, Giulio Jori Series eds.), Elsevier Science, Amsterdam (in press)

  7. I. Mellon V. A. Bohr C. A. Smith P. C. Hanawalt (1986) ArticleTitlePreferential DNA repair of an active gene in human cells Proc. Natl. Acad. Sci. USA 83 8878–8882 Occurrence Handle3466163

    PubMed  Google Scholar 

  8. I. Mellon G. Spivak P. C. Hanawalt (1987) ArticleTitleSelective removal of transcription-blocking DNA damage from the transcribed strand of the mammalian DHFR gene Cell 51 241–249 Occurrence Handle10.1016/0092-8674(87)90151-6 Occurrence Handle3664636

    Article  PubMed  Google Scholar 

  9. J. Venema A. Hoffen Particlevan A. T. Natarajan A. A. Zeeland Particlevan L. H. Mullenders (1990) ArticleTitleThe residual repair capacity of xeroderma pigmentosum complementation group C fibroblasts is highly specific for transcriptionally active DNA Nucleic Acids Res. 18 443–448 Occurrence Handle2308842

    PubMed  Google Scholar 

  10. J. Venema A. Hoffen Particlevan V. Karcagi A. T. Natarajan A. A. Zeeland Particlevan L. H. Mullenders (1991) ArticleTitleXeroderma pigmentosum complementation group C cells remove pyrimidine dimers selectively from the transcribed strand of active genes Mol. Cell Biol. 11 4128–4134 Occurrence Handle1649389

    PubMed  Google Scholar 

  11. J. Venema L. H. Mullenders A. T. Natarajan A. A. Zeeland Particlevan L. V. Mayne (1990) ArticleTitleThe genetic defect in Cockayne syndrome is associated with a defect in repair of UV-induced DNA damage in transcriptionally active DNA Proc. Natl. Acad. Sci. USA 87 4707–4711 Occurrence Handle2352945

    PubMed  Google Scholar 

  12. M. A. Francis A. J. Rainbow (1999) ArticleTitleUV-enhanced reactivation of a UV-damaged reporter gene suggests transcription-coupled repair is UV inducible in human cells Carcinogenesis 20 19–26 Occurrence Handle10.1093/carcin/20.1.19 Occurrence Handle9934845

    Article  PubMed  Google Scholar 

  13. B. C. McKay M. A. Francis A. J. Rainbow (1997) ArticleTitleWild type p53 is required for heat shock and ultraviolet light enhanced repair of a UV-damaged reported gene Carcinogenesis 18 245–249 Occurrence Handle10.1093/carcin/18.2.245 Occurrence Handle9054614

    Article  PubMed  Google Scholar 

  14. G. Li V. C. Ho (1998) ArticleTitlep53-dependent DNA repair and apoptosis respond differently to high and low dose ultraviolet radiation Br. J. Dermatol. 139 3–10 Occurrence Handle10.1046/j.1365-2133.1998.02306.x

    Article  Google Scholar 

  15. I. P. Boszko A. J. Rainbow (2002) ArticleTitleRemoval of UV photoproducts from an adenovirus-encoded reporter gene following infection of unirradiated and UV-irradiated human fibroblasts Somat. Cell Mol. Genet. 25 301–315 Occurrence Handle10.1023/A:1019916415806

    Article  Google Scholar 

  16. J. Huang N. Logsdon F. I. Schmieg D. T. Simmons (1998) ArticleTitlep53-mediated transcription induces resistance of DNA to UV inactivation Oncogene 17 401–411 Occurrence Handle10.1038/sj.onc.1201951 Occurrence Handle9696032

    Article  PubMed  Google Scholar 

  17. M. Smith I. T. Chen Q. Zhan I. Bae C. Y. Chen T. M. Gilmer M. B. Kastan P. M. O’Connor A. J. Fornace SuffixJr (1994) ArticleTitleInteraction of the p53-regulated protein Gadd45 with proliferating cell nuclear antigen Science 266 1376–1380 Occurrence Handle7973727

    PubMed  Google Scholar 

  18. M. L. Smith I. T. Chen Q. Zhan P. M. O’Connor A. J. Fornace SuffixJr (1995) ArticleTitleInvolvement of the p53 tumor suppressor in repair of UV-type DNA damage Oncogene 10 1053–1059 Occurrence Handle7700629

    PubMed  Google Scholar 

  19. J. M. Ford P. C. Hanawalt (1995) ArticleTitleLi-Fraumeni syndrome fibroblasts homozygous for p53 mutations are deficient in global DNA repair but exhibit normal transcription-coupled repair and enhanced UV resistance Proc. Natl. Acad. Sci. USA 92 8876–8880 Occurrence Handle7568035

    PubMed  Google Scholar 

  20. J. M. Ford P. C. Hanawalt (1997) ArticleTitleExpression of wild-type p53 is required for efficient global nucleotide excision repair in UV-irradiated human fibroblasts J. Biol. Chem. 272 28073–28080 Occurrence Handle10.1074/jbc.272.44.28073 Occurrence Handle9346961

    Article  PubMed  Google Scholar 

  21. J. M. Ford E. L. Baron P. C. Hanwalt (1998) ArticleTitleHuman fibroblasts expressing the human papillomavirus E6 gene are deficient in global genomic nucleotide excision repair and sensitive to ultraviolet irradiation Cancer Res. 58 599–603 Occurrence Handle9485006

    PubMed  Google Scholar 

  22. J. P. Therrien R. Drouin C. Baril E. A. Drobetsky (1999) ArticleTitleHuman cells compromised for p53 function exhibit defective global and transcription-coupled nucleotide excision repair, whereas cells compromised for Rb function are defective only in global repair Proc. Natl. Acad. Sci. USA 96 15038–15043 Occurrence Handle10.1073/pnas.96.26.15038 Occurrence Handle10611334

    Article  PubMed  Google Scholar 

  23. G. Mathonnet C. Leger J. Desnoyers R. Drouin J. P. Therrien E. A. Drobetsky (2003) ArticleTitleUV-wavelength dependent regulation of transcription-coupled nucleotide excision repair in p53-deficient human cells Proc Natl Acad. Sci. USA 100 7219–7224 Occurrence Handle10.1073/pnas.1232161100 Occurrence Handle12775760

    Article  PubMed  Google Scholar 

  24. B. C. McKay M. Ljungman A. J. Rainbow (1999) ArticleTitlePotential roles for p53 in nucleotide excision repair Carcinogenesis 20 1389–1396 Occurrence Handle10.1093/carcin/20.8.1389 Occurrence Handle10426782

    Article  PubMed  Google Scholar 

  25. M. L. Smith R. S. Young (2002) ArticleTitleP53 regulation of DNA excision repair pathways Mutagenesis 17 149–156 Occurrence Handle10.1093/mutage/17.2.149 Occurrence Handle11880544

    Article  PubMed  Google Scholar 

  26. M. L. Smith H. U. Kontny Q. Zhan A. Sreenath P. M. O’Connor A. J. Fornace SuffixJr (1996) ArticleTitleAntisense GADD45 expression results in decreased DNA repair and sensitizes cells to UV-irradiation or cisplatin Oncogene 13 2255–2263 Occurrence Handle8950993

    PubMed  Google Scholar 

  27. B. J. Hwang S. Toering U. Francke G. Chu (1998) ArticleTitlep48 activates a UV-damaged-DNA binding factor and is defective in xeroderma pigmentosum group E cells that lack binding activity Mol. Cell. Biol. 18 4391–4399 Occurrence Handle9632823

    PubMed  Google Scholar 

  28. B. J. Hwang J. M. Ford P. C. Hanawalt G. Chu (1999) ArticleTitleExpression of the p48 xeroderma pigmentosum gene is p53-dependent and involved in global genomic repair Proc. Natl. Acad. Sci. USA 96 424–428

    Google Scholar 

  29. T. Tan G. Chu (2002) ArticleTitleExpression of the p48 xeroderma pigmentosum gene is p53-dependent and involved in global genomic repair Mol. Cell. Biol. 22 3247–3254 Occurrence Handle10.1128/MCB.22.10.3247-3254.2002 Occurrence Handle11971958

    Article  PubMed  Google Scholar 

  30. M. E. Fitch I. V. Cross S. T. Turner S. Adimoolam C. X. Lin K. G. Williams J. M. Ford (2003) ArticleTitleThe DDB2 nucleotide excision repair gene product p48 enhances global genomic repair in p53 deficient human fibroblasts DNA Repair 2 819–826 Occurrence Handle10.1016/S1568-7864(03)00066-1 Occurrence Handle12826282

    Article  PubMed  Google Scholar 

  31. M. E. Fitch I. V. Cross J. M. Ford (2003) ArticleTitlep53 responsive nucleotide excision repair gene products p48 and XPC, but not p53, localize to sites of UV-irradiation-induced DNA damage, in vivo Carcinogenesis 24 843–850 Occurrence Handle10.1093/carcin/bgg031 Occurrence Handle12771027

    Article  PubMed  Google Scholar 

  32. S. A. Amundson K. T. Do S. Shahab M. Bittner P. Meltzer J. Trent Fornace SuffixJr. A. J. (2000) ArticleTitleIdentification of potential mRNA biomarkers in peripheral blood lymphocytes for human exposure to ionizing radiation Radiat. Res. 154 342–346 Occurrence Handle11012342

    PubMed  Google Scholar 

  33. S. A. Amundson A. Patterson K. T. Do A. J. Fornace SuffixJr (2002) ArticleTitleA nucleotide excision repair master-switch: p53 regulated coordinate induction of global genomic repair genes Cancer Biol. Ther. 1 145–149 Occurrence Handle12170774

    PubMed  Google Scholar 

  34. S. Adimoolam J. M. Ford (2002) ArticleTitlep53 and DNA damage-inducible expression of the xeroderma pigmentosum group C gene Proc. Natl. Acad. Sci. USA 99 12985–12990 Occurrence Handle10.1073/pnas.202485699 Occurrence Handle12242345

    Article  PubMed  Google Scholar 

  35. B. C. McKay M. Ljungman A. J. Rainbow (1998) ArticleTitlePersistent DNA damage induced by ultraviolet light inhibits p21waf1 and bax expression: implications for DNA repair, UV sensitivity and the induction of apoptosis Oncogene 17 545–555 Occurrence Handle10.1038/sj.onc.1201963 Occurrence Handle9704920

    Article  PubMed  Google Scholar 

  36. A. Collins (1993) ArticleTitleMutant rodent cell lines sensitive to ultraviolet light, ionizing radiation and cross-linking agents: a comprehensive survey of genetic and biochemical characteristics Mutat. Res. 293 99–118 Occurrence Handle7678147

    PubMed  Google Scholar 

  37. A. Collins R. T. Johnson (1987) ArticleTitleDNA repair mutants in higher eukaryotes J. Cell Sci. 6 IssueIDSuppl. 61–82

    Google Scholar 

  38. N. J. Jones (1994) ArticleTitleGenetic analysis of mitomycin C-hypersensitive Chinese hamster cell mutant Mutagenesis 9 477–482 Occurrence Handle7837983

    PubMed  Google Scholar 

  39. D. K. Orren G. L. Dianov V. A. Bohr (1996) ArticleTitleThe human CSB(ERCC6) gene corrects the transcription coupled repair defect in the CHO cell mutant UV61 Nucleic Acids Res. 24 3317–3322 Occurrence Handle10.1093/nar/24.17.3317 Occurrence Handle8811084

    Article  PubMed  Google Scholar 

  40. C. Troelstra H. Odijk J. Wit Particlede A. Westerveld L. H. Thompson D. Bootsma J. H. J. Hoeijmakers (1990) ArticleTitleMolecular cloning of the human DNA excision repair gene ERCC6 Mol. Cell. Biol. 10 5806–5813 Occurrence Handle2172786

    PubMed  Google Scholar 

  41. V. A. Bohr C. A. Smith D. S. Okumoto P. C. Hanawalt (1985) ArticleTitleDNA repair in an active gene: removal of pyrimidine dimmers from the DHFR gene of CHO cells is much more efficient than in the genome overall Cell 40 359–369 Occurrence Handle10.1016/0092-8674(85)90150-3 Occurrence Handle3838150

    Article  PubMed  Google Scholar 

  42. V. A. Bohr D. S. Okumoto P. C. Hanawalt (1986) ArticleTitleSurvival of UV-irradiated mammalian cells correlates with efficient DNA repair in an essential gene Proc. Natl. Acad. Sci. USA 83 3830–3833 Occurrence Handle3459159

    PubMed  Google Scholar 

  43. L. H. Thompson D. L. Mitchell J. D. Regan S. D. Bouffler S. A. Stewart W. L. Carrier R. S. Nairn R. T. Johnson (1989) Mutagenesis 4 140–146 Occurrence Handle2659925

    PubMed  Google Scholar 

  44. L. Lommel P. C. Hanawalt (1991) ArticleTitleThe genetic defect in the Chinese hamster ovary cell mutant UV61 permits moderate selective repair of cyclobutane pyrimidine dimers in an expressed gene Mutat. Res. 255 183–191 Occurrence Handle1922150

    PubMed  Google Scholar 

  45. A. S. Balajee L. P. DeSantis R. M. Brosh R. Selzer V. A. Bohr (2000) ArticleTitleRole of the ATPase domain of the Cockayne syndrome group B protein in UV-induced apoptosis Oncogene 19 477–489 Occurrence Handle10.1038/sj.onc.1203372 Occurrence Handle10698517

    Article  PubMed  Google Scholar 

  46. W. R. G. Arnold A. J. Rainbow (1996) ArticleTitleHost cell reactivation of irradiated adenovirus in UV sensitive Chinese hamster ovary cell mutants Mutagenesis 11 89–94 Occurrence Handle8671722

    PubMed  Google Scholar 

  47. M. Germanier M. Defais V. A. Bohr F. Larminat (2000) ArticleTitleTranscription-coupled repair is inducible in hamster cells Nucleic Acids Res. 28 4674–4678 Occurrence Handle10.1093/nar/28.23.4674 Occurrence Handle11095677

    Article  PubMed  Google Scholar 

  48. L. H. Thompson S. Fong K. Brookman (1980) ArticleTitleValidation of conditions for efficient detection of hprt and aprt mutations in suspension-cultured Chinese hamster ovary cells Mutat. Res. 74 21–36 Occurrence Handle7360155

    PubMed  Google Scholar 

  49. L. H. Thompson J. S. Rubin J. E. Cleaver G. F. Whitmore K. Brookman (1980) ArticleTitleA screening method for isolating DNA repair-deficient mutants of CHO cells Somat. Cell Genet. 6 391–405 Occurrence Handle10.1007/BF01542791 Occurrence Handle7404270

    Article  PubMed  Google Scholar 

  50. C. L. Addison M. M. Hitt D. Kunsken F. L. Graham (1997) ArticleTitleComparison of the human versus murine cytomegalovirus immediate early promoters for transgene expression by adenoviral vectors J. Gen. Virol. 78 1653–1661 Occurrence Handle9225042

    PubMed  Google Scholar 

  51. Graham, F. L. and Prevec, L. (1991) Manipulation of adenovirus vectors. In: Gene Transfer and Expression Protocols. (E. J. Murray ed.) The Human press Inc., Clifton, NJ, 7, 109–128

  52. L. C. Santis ParticleDe C. L. Garcia A. D. Balajee P. Latini P. Pichierri O. Nikaido M. Stefanini F. Palitti (2002) ArticleTitleTranscription coupled repair efficiency determines the cell cycle progression and apoptosis after UV exposure in hamster cells DNA Repair 1 209–223 Occurrence Handle10.1016/S1568-7864(01)00017-9 Occurrence Handle12509253

    Article  PubMed  Google Scholar 

  53. M. A. Francis A. J. Rainbow (2000) ArticleTitleUV-enhanced expression of a reporter gene is induced at lower UV fluences in transcription-coupled repair deficient compared to normal human fibroblasts, and is absent in SV40-transformed counterparts Photochem. Photobiol. 72 554–561 Occurrence Handle10.1562/0031-8655(2000)072<0554:UEEOAR>2.0.CO;2 Occurrence Handle11045729

    Article  PubMed  Google Scholar 

  54. L. V. Mayne A. R. Lehmann (1982) ArticleTitleFailure of RNA synthesis to recover after UV irradiation: an early defect in cells from individuals with Cockayne’s syndrome and xeroderma pigmentosum Cancer Res. 42 1473–1478 Occurrence Handle6174225

    PubMed  Google Scholar 

  55. M. Protic-Sabljic K. H. Kraemer (1985) ArticleTitleOne pyrimidine dimer inactivates expression of a transfected gene in xeroderma pigmentosum cells Proc. Natl. Acad. Sci. USA 82 6622–6626 Occurrence Handle2995975

    PubMed  Google Scholar 

  56. B. A. Donahue S. Yin J. S. Taylor D. Reines P. C. Hanawalt (1994) ArticleTitleTranscript cleavage by RNA polymerase arrested by a cyclobutane pyrimidine dimer in the DNA template Proc. Natl. Acad. Sci. USA 91 8502–8506 Occurrence Handle8078911

    PubMed  Google Scholar 

  57. B. C. McKay A. J. Rainbow (1996) ArticleTitleHeat-shock enhanced reactivation of a UV-damaged reported gene in human cells involves the transcription coupled DNA repair pathway Mutat. Res. 363 125–135 Occurrence Handle8676926

    PubMed  Google Scholar 

  58. H. Lee J. M. Larner J. L. Hamlin (1997) ArticleTitleCloning and characterization of Chinese hamster p53 cDNA Gene 184 177–183 Occurrence Handle10.1016/S0378-1119(96)00592-6 Occurrence Handle9031625

    Article  PubMed  Google Scholar 

  59. D. Walker J. P. Bond R. E. Tarone C. C. Harris W. Makalowski M. S. Boguski M. S. Greenblatt (1999) ArticleTitleEvolutionary conservation and somatic mutation hotpot map of p53: correlation with p53 protein structural and functional features Oncogene 19 211–218 Occurrence Handle10.1038/sj.onc.1202298

    Article  Google Scholar 

  60. B. Tzang Y. Lai M. Hsu H. Chang C. Chang P. Huang Y. Liu (1999) ArticleTitleFunction and sequence analyses of tumor suppressor gene p53 of CHO.K1 cells Cell Biol. 18 315–321

    Google Scholar 

  61. M. L. Smith J. M. Ford M. C. Hollander R. A. Bortnick S. A. Amundson Y. R. Seo C. Deng P. C. Hanawalt A. J. Fornace (2000) ArticleTitlep53-Mediated DNA repair response to UV radiation: Studies of mouse cell lacking p53, p21, and/or gadd45 gene Mol. Cell. Biol. 2000 3705–3714 Occurrence Handle10.1128/MCB.20.10.3705-3714.2000

    Article  Google Scholar 

  62. M. Yamaizumi T. Sugano (1994) ArticleTitleUV-induced nuclear accumulation of p53 is evoked through DNA damage of actively transcribed genes independent of the cell cycle Oncogene 9 2775–2784 Occurrence Handle8084582

    PubMed  Google Scholar 

  63. M. Ljungman F. Zhang (1996) ArticleTitleBlockage of RNA polymerase as a possible trigger for UV. light-induced apoptosis Oncogene 13 823–831 Occurrence Handle8761304

    PubMed  Google Scholar 

  64. M. Ljungman F. Zhang F. Chen A. J. Rainbow B. C. McKay (1999) ArticleTitleInhibition of RNA polymerase II as a trigger for the p53 response Oncogene 18 583–592 Occurrence Handle10.1038/sj.onc.1202356 Occurrence Handle9989808

    Article  PubMed  Google Scholar 

  65. L. J. Ko C. Prives (1996) ArticleTitlep53: puzzle and paradigm Genes Dev. 1 1054–1072

    Google Scholar 

  66. C. Sachsenmaier A. Radler-Pohl R. Zinck A. Nordheim P. Herrlich H. J. Rahmsdorf (1994) ArticleTitleInvolvement of growth factor receptors in the mammalian UVC response Cell 78 963–972 Occurrence Handle10.1016/0092-8674(94)90272-0 Occurrence Handle7923365

    Article  PubMed  Google Scholar 

  67. K. Bender C. Blattner A. Knebel M. Iordanov P. Herrlich H. J. Rahmsdorf (1997) ArticleTitleUV-induced signal transduction J. Photochem. Photobiol. B: Biol. 37 1–17 Occurrence Handle10.1016/S1011-1344(96)07459-3

    Article  Google Scholar 

  68. M. Schorpp U. Mallick H. J. Rahmsdorf P. Herrlich (1984) ArticleTitleUV-induced extracellular factor from human fibroblasts communicates the UV response to nonirradiated cells Cell 37 861–868 Occurrence Handle10.1016/0092-8674(84)90421-5 Occurrence Handle6744414

    Article  PubMed  Google Scholar 

  69. B. Stein H. J. Rahmsdorf A. Steffen M. Litfin P. Herrlich (1989) ArticleTitleUV-induced DNA damage is an intermediate step in UV-induced expression of human immunodeficiency virus type 1, collagenase, c-fos, and metallothionein Mol. Cell. Biol. 9 5169–5181 Occurrence Handle2557547

    PubMed  Google Scholar 

  70. C. Blattner K. Bender P. Herrlich H. J. Rahmsdorf (1998) ArticleTitlePhotoproducts in transcriptionally active DNA induce signal transduction to the delayed U.V.-responsive genes for collagenase and metallothionein Oncogene 16 2827–2834 Occurrence Handle10.1038/sj.onc.1201827 Occurrence Handle9671403

    Article  PubMed  Google Scholar 

  71. B. Stein M. Kramer H. J. Rahmsdorf H. Ponta P. Herrlich (1989) ArticleTitleUV-induced transcription from the human immunodeficiency virus type 1 (HIV-1) long terminal repeat and UV-induced secretion of an extracellular factor that induces HIV-1 transcription in nonirradiated cells J. Virol. 63 4540–4544 Occurrence Handle2795711

    PubMed  Google Scholar 

  72. P. Herrlich H. Ponta H. J. Rahmsdorf (1992) ArticleTitleDNA damage-induced gene expression: signal transduction and relation to growth factor signaling Rev. Physiol. Biochem. Pharmacol. 119 187–223 Occurrence Handle1604153

    PubMed  Google Scholar 

  73. M. Kramer C. Sachsenmaier P. Herrlich H. J. Rahmsdorf (1993) ArticleTitleUV irradiation-induced interleukin-1 and basic fibroblast growth factor synthesis and release mediate part of the UV response J. Biol. Chem. 268 6734–6741 Occurrence Handle8454646

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology, McMaster University, L8S 4K1, Hamilton, Ontario, Canada

    Lili Liu & Andrew J. Rainbow

Authors
  1. Lili Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Andrew J. Rainbow
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Andrew J. Rainbow.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Liu, L., Rainbow, A.J. Pre-UV-Treatment of Cells Results in Enhanced Host Cell Reactivation of a UV Damaged Reporter Gene in CHO-AA8 Chinese Hamster Ovary Cells but Not in Transcription-Coupled Repair Deficient CHO-UV61 Cells. Biosci Rep 24, 559–576 (2004). https://doi.org/10.1007/s10540-005-2792-x

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10540-005-2792-x

Keywords

Search

Navigation