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Comparison of sucrose metabolism in wheat seedlings during drought stress and subsequent recovery

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Biologia Plantarum

Abstract

Sucrose is a dominant sugar transported to the sink organs of a plant where it is metabolized to other compounds or stored. Here, the importance of sucrose metabolism in a drought-tolerant wheat cultivar was compared to a drought-sensitive one. The 4-d-old Triticum aestivum L. seedlings were exposed to drought induced by water cessation for 7 d and recovery after re-watering for further 7 d. Under control conditions, constitutive expression of genes encoding vacuolar invertase (VI) and sucrose synthase (SuS) and activity of sucrose phosphate synthase (SPS) were significantly higher in the tolerant cultivar than in the sensitive one. Drought promoted the expressions of SPS and VI genes in the tolerant cultivar and increased their activities to 175 and 132 %, respectively, of those under control conditions. The activity of SuS and expression of its gene, however, were identical in both cultivars under drought stress. These changes resulted in more remarkable accumulation of sucrose in tolerant than in sensitive cultivar under water stress.

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Abbreviations

NI:

neutral invertase

SPP:

sucrose-phosphate phosphatase

SPS:

sucrose phosphate synthase

SuS:

sucrose synthase

UDP:

uridine diphosphate

VI:

vacuolar invertase

References

  • Bogdan, J., Zagdanska, B.: Alterations in sugar metabolism coincide with a transition of wheat seedlings to dehydration intolerance. - Environ. exp. Bot. 66: 186–194, 2009.

    Article  CAS  Google Scholar 

  • Bonfig, K.B., Gabler, A., Simon, U.K., Luschin-Ebengreuth, N., Hatz, M., Berger, S., Muhamma, N., Zeier, J., Sinha, A.K., Roitsch, T.: Post-translational derepression of invertase activity in source leaves via down-regulation of invertase inhibitor expression is part of the plant defense response. - Mol. Plant 3: 1037–1048, 2010.

    Article  PubMed  CAS  Google Scholar 

  • Castleden, C.K., Aoki, N., Gillespie, V.J., Mac Rae, E.A., Quick, W.P., Buchner, P., Foyer, C.H., Furbank, R.T., Lunn, J.E.: Evolution and function of the sucrose-phosphate synthase gene families in wheat and other grasses. - Plant Physiol. 135: 1753–1764, 2004.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Devi, M., Tenaud, P., Champigny, M.L.: A comparative study on the soluble invertase activities and sucrose content in different clones of sugar cane. - In: Sybesma, C. (ed.): Advances in Photosynthesis Research. Advances in Agricultural Biotechnology. Vol. 4. Pp. 881–884. Springer, Dordrecht 1984.

    Article  CAS  Google Scholar 

  • Djilianov, D., Ivanov, S., Moyankova, D., Miteva, L., Kirova, E., Alexieva, V., Joudi, M., Peshev, D., Van den Ende, W.: Sugar ratios, glutathione redox status and phenols in the resurrection species Haberlea rhodopensis and the closely related non-resurrection species Chirita eberhardtii. - Plant Biol. 13: 767–776, 2011.

    Article  PubMed  CAS  Google Scholar 

  • Fu, J., Huang, B., Fry, J.: Osmotic potential, sucrose level, and activity of sucrose metabolic enzymes in tall fescue in response to deficit irrigation. - J. amer. Soc. hort. Sci. 135: 506–510, 2010.

    Google Scholar 

  • Hammond, J.P., White, P.J.: Sugar signaling in root response to low phosphorus availability. - Plant Physiol. 156:1033–1040, 2011.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Huber, S.C., Rufty, T.W., Kerr, P.S.: Effect of photoperiod on photosynthate partitioning and diurnal rhythms in sucrose phosphate synthase activity in leaves of soybean (Glycine max L. [Merr.]) and tobacco (Nicotiana tabacum L.). - Plant Physiol. 75: 1080–1084, 1984.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Koch, K.: Sucrose metabolism: regulatory mechanisms and pivotal roles in sugar sensing and plant development. - Curr. Opin. Plant Biol. 7: 235–246, 2004.

    Article  PubMed  CAS  Google Scholar 

  • Koobaz, P., Ghanati, F., Hosseini Salekdeh, G., Moradi, F., Hadavand, H.: Drought tolerance in four-day-old seedlings of a drought-sensitive cultivar of wheat. - J. Plant Nutr. 40: 574–583, 2017.

    Article  CAS  Google Scholar 

  • Koster, K.L.: Glass formation and desiccation tolerance in seeds. - Plant Physiol. 96: 302–304, 1991.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Liao, W.B., Li, Y.Y., Lu, C., Peng, M.: Expression of sucrose metabolism and transport genes in cassava petiole abscission zones in response to water stress. - Biol. Plant. 61: 219–226, 2017.

    Article  CAS  Google Scholar 

  • Marafla, C., Garcia-Olmedo, F., Carbenero, P.: Differential expression of two types of sucrose synthase-encoding genes in wheat in response to anaerobiosis, cold shock and light. - Gene 88: 167–172, 1990.

    Article  Google Scholar 

  • Medici, A., Laloi, M., Atanassova, R.: Profiling of sugar transporter genes in grapevine coping with water deficit. - FEBS Lett. 588: 3989–3997, 2014.

    Article  PubMed  CAS  Google Scholar 

  • Merlo, L., Passera, C.: Changes in carbohydrate and enzyme levels during development of leaves of Prunus persica, a sorbitol synthesizing species. - Physiol. Plant. 83: 621–626, 1991.

    Article  CAS  Google Scholar 

  • Miazek, A., Bogdan, J., Zagdanska, B.: Effects of water deficit during germination of wheat seeds. - Biol. Plant. 44: 397–403, 2001.

    Article  Google Scholar 

  • Pour-Aboughadareh, A., Ahmadi, J., Mehrabi, A.A., Etminan, A., Moghaddam, M., Siddique, K.H.: Physiological responses to drought stress in wild relatives of wheat: implications for wheat improvement. - Acta Physiol. Plant. 39: 1–16, 2017.

    Article  CAS  Google Scholar 

  • Roitsch, T., Balibrea, M.E., Hofmann, M., Proels, R., Sinha, A.K.: Extracellular invertases: key metabolic enzyme and PR protein. - J. exp. Bot. 54: 513–24, 2003.

    Article  PubMed  CAS  Google Scholar 

  • Roitsch, T., Gonzalez, M.C.: Function and regulation of plant invertases: sweet sensations. - Trends Plant Sci. 9: 606–613, 2004.

    Article  PubMed  CAS  Google Scholar 

  • Rosa, M., Prado, C., Podazza, G., Interdonato, R., Gonzalez, J.A., Hilal, M. Prado F.E.: Soluble sugars, metabolism, sensing and abiotic stress. - Plant Signal. Behav. 4: 388–393, 2009.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Saeedipour, S., Moradi, F.: Comparison of the drought stress responses of tolerant and sensitive wheat cultivars during grain filling: impact of invertase activity on carbon metabolism during kernel development. - J. agr. Sci. 3: 32–44, 2011.

    Google Scholar 

  • Verspreet, J., Cimini, S., Vergauwen, R., Dornez, E., Locato, V., Le Roy, K., De Gara, L., Van den Ende, W., Delcour, J.A., Courtin, C.M.: Fructan metabolism in developing wheat (Triticum aestivum L.) kernels. - Plant Cell Physiol. 54: 2047–2057, 2013.

    Article  PubMed  CAS  Google Scholar 

  • Verspreet, J., Pollet, A., Cuyvers, S., Vergauwen, R., Van den Ende, W., Delcour, J.A., Courtin, C.M.: A simple and accurate method for determining wheat grain fructan content and average degree of polymerization. - J. agr. Food Chem. 60: 2102–2107, 2012.

    Article  CAS  Google Scholar 

  • Winter, H., Huber, S.C.: Regulation of sucrose metabolism in higher plants: localization and regulation of activity of key enzymes. - Crit. Rev. Biochem. mol. Biol. 35: 253–289, 2000.

    Article  PubMed  CAS  Google Scholar 

  • Yu, S.M., Lo, S.F., Ho, T.H.D.: Source-sink communication: regulated by hormone, nutrient, and stress cross signaling. - Trends Plant Sci. 20: 844–857, 2015

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Plant Biology, Faculty of Biological Science, Tarbiat Modares University, 14115-154, Tehran, Iran

    F. Nemati, F. Ghanati & M. Sharifi

  2. Department of Food Science and Technology, Faculty of Agriculture, Tarbiat Modares University, 14115-336, Tehran, Iran

    H. Ahmadi Gavlighi

Authors
  1. F. Nemati
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  2. F. Ghanati
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  3. H. Ahmadi Gavlighi
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  4. M. Sharifi
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Corresponding author

Correspondence to F. Ghanati.

Additional information

Acknowledgments: The authors would like to thank Tarbiat Modares University for providing the laboratory facilities and financial support for this project.

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Nemati, F., Ghanati, F., Ahmadi Gavlighi, H. et al. Comparison of sucrose metabolism in wheat seedlings during drought stress and subsequent recovery. Biol Plant 62, 595–599 (2018). https://doi.org/10.1007/s10535-018-0792-5

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  • DOI: https://doi.org/10.1007/s10535-018-0792-5

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