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Biologia Plantarum

, Volume 62, Issue 1, pp 69–79 | Cite as

High irradiance sensitive phenotype of Arabidopsis hit2/xpo1a mutant is caused in part by nuclear confinement of AtHsfA4a

  • H.-Y. Huang
  • K.-Y. Chang
  • S.-J. Wu
Original papers
  • 34 Downloads

Abstract

In Arabidopsis, EXPORTIN1A (HIT2/XPO1A) and EXPORTIN1B (XPO1B) mediate the translocation of nuclear export sequence (NES)-bearing proteins from nucleus to cytoplasm. However, a mutation in HIT2/XPO1A but not in XPO1B induces sensitivity to high irradiance (HI). Arabidopsis thaliana heat stress elements A4a and A5 (AtHsfA4a and AtHsfA5) are involved in plant responses to HI and possess NESs; therefore, their nucleo-cytoplasmic partitioning was analyzed. In wild-type and xpo1b mutant cells, AtHsfA4a normally remained in the cytoplasm but became concentrated in the nucleus following exposure to HI, whereas AtHsfA5 was constitutively distributed in both cytoplasm and nucleus. However, in hit2/xpo1a mutant, AtHsfA4a and AtHsfA5 were always confined to the nucleus, regardless of the irradiance. Although AtHsfA4a can enhance the ability of plants to scavenge H2O2, and AtHsfA5 is a repressor of AtHsfA4a, athsfa5 but not athsfa4a mutant plants exhibited HI sensitivity. Additionally, athsfa4a plants expressing AtHsfA4aΔNES were sensitive to HI, but athsfa5 plants expressing AtHsfA5ΔNES were not. Meanwhile, hit2/athsfa4a double mutant was more tolerant to HI than hit2. These results indicate that both AtHsfA4a and AtHsfA5 were HIT2/XPO1A-specific substrates. Long-term accumulation of AtHsfA4a contributed to the hit2 HI-sensitive phenotype independent of the scavenging ability of H2O2, and the presence of AtHsfA5 could mitigate this adverse effect.

Additional key words

exportin1 (XPO1) heat stress factor A4a nuclear transport receptor 

Abbreviations

APX

ascorbate peroxidase

BiFC

bimolecular fluorescent complementation

CAT

catalase

GFP

green fluorescent protein

Hsf

heat stress factor

HSE

heat stress element

HSP

heat shock protein

LMB

leptomycin B

MV

methyl viologen

NES

nuclear export sequence

NLS

nuclear localization sequence

ROS

reactive oxygen species

SOD

superoxide dismutase

WT

wild-type

XPO1

exportin1

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Copyright information

© The Institute of Experimental Botany 2018

Authors and Affiliations

  1. 1.Department of Life SciencesNational Central UniversityTaoyuan CityTaiwan

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