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Biologia Plantarum

, Volume 62, Issue 1, pp 166–172 | Cite as

The effects of silver ions and silver nanoparticles on cell division and expression of cdc2 gene in Allium cepa root tips

Original papers
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Abstract

The effects of silver nanoparticles (AgNPs), silver ions (Ag+), and polyvinylpyrrolidone (PVP) on mitosis and expression of a gene encoding cyclin-dependent kinase 2 (cdc2) in onion roots were compared. Three concentrations (5, 10, and 15 mg dm-3) were employed in combination with three incubation times (3, 6, and 9 h). PVP enhanced mitotic index and cdc2 expression. Both silver forms decreased mitotic index and cdc2 expression. Genotoxicity of both silver forms were indicated by three major distinguishable classes of chromosome aberrations: spindle disturbances, clastogenic aberrations, and chromosome stickiness. Concerning Ag+ treatments, significant enhancements in occurrence of any chromosome aberration type was associated with significant decrease in mitotic index. On the other hand, disturbed spindle in AgNPs treatments was observed even in absence of significant reduction in mitotic index suggesting that AgNPs inhibit cellular events occurring during mitosis to proceed normally rather than starting of cell division.

Additional key words

chromosome aberrations mitotic index mitotic abnormalities phase index real-time PCR 

Abbreviations

Ag+

silver ion

AgNPs

silver nanoparticles

CDKs

cyclin-dependent kinases

CKL

cyclin dependent kinase like

CYC

cyclin

DW

deionized water

ENPs

engineered nanoparticles

LSD

least significant difference

PVP

polyvinylpyrrolidone

TEM

transmission electron microscopy

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Supplementary material

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Copyright information

© The Institute of Experimental Botany 2018

Authors and Affiliations

  1. 1.Botany and Microbiology Department, Faculty of ScienceCairo UniversityCairoEgypt

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