Comparison of nitrogen uptake in the roots and rhizomes of Leymus chinensis
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Leymus chinensis (Trin.) Tzvel is a rhizomatous grass species in the Eastern Eurasian steppe zone that is often limited by low soil nitrogen availability. Although a previous study showed that the rhizomes of L. chinensis have the capacity to take up nitrogen, the importance of such uptake for nitrogen nutrition is unclear. Moreover, little is known regarding the inorganic nitrogen uptake kinetics of roots and rhizomes in response to nitrogen status. Here, we first found that ammonium is preferred over nitrate and glycine for L. chinensis growth. Using the 15N-labelling method, we found that the rate of ion influx into roots was approximately five-fold higher than into rhizomes under the same nitrogen content, and the ion influxes into roots and rhizomes under 0.05 mM N were greater than in the presence of 3 mM N, especially in the form of NH4+. Using a non-invasive micro-test technique, we characterised the patterns of NH4+ and NO3– fluxes in the root mature zone, root tip, rhizome mature zone, and rhizome tip following incubation in the solution with different N compounds and different N concentrations. These results suggest a dynamic balance between the uptake, utilisation, and excretion of nitrogen in L. chinensis.
Additional key wordsion fluxes HATS LATS 15N-labelling non-invasive micro-test
ammonium transport proteins
high-affinity transport system
low-affinity transport system
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- Chen, S., Huang, X., Yan, X., Liang, Y., Wang, Y., Li, X., Peng, X., Ma, X., Zhang, L., Cai, Y., Ma, T., Cheng, L., Qi, D., Zheng, H., Yang, X., Li, X., Liu, G.: Transcriptome analysis in sheepgrass (Leymus chinensis): a dominant perennial grass of the eurasian steppe. — PLoS ONE 8: e67974, 2013b.CrossRefGoogle Scholar
- Crawford, N.M., Glass, A.D.M.: Molecular and physiological aspects of nitrate uptake in plants. — Trends Plant Sci. 3: 1360–1385, 1998.Google Scholar
- Elashry, A., Okumoto, S., Siddique, S., Koch, W., Kreil, D.P., Bohlmann, H.: The AAP gene family for amino acid permeases contributes to development of the cyst nematode Heterodera schachtii in roots of Arabidopsis. — Plant Physiol. Biochem. 70: 379–386, 2013.CrossRefPubMedPubMedCentralGoogle Scholar
- Henriksen, G.H., Raman, D.R., Walker, L.P., Spanswick, R.M.: Measurement of net fluxes of ammonium and nitrate at the surface of barley roots using ion-selective microelectrodes: II. patterns of uptake along the root axis and evaluation of the microelectrode flux estimation technique. — Plant Physiol. 99: 734–747, 1992.CrossRefPubMedPubMedCentralGoogle Scholar
- Ju, X.-T., Xing, G.-X., Chen, X.-P., Zhang, S.-L., Zhang, L.-J., Liu, X.-J., Cui, Z.-L., Yin, B., Christie, P., Zhu, Z.-L., Zhang, F.-S.: Reducing environmental risk by improving N management in intensive Chinese agricultural systems. — Proc. nat. Acad. Sci. USA 106: 3041–3046, 2009.CrossRefPubMedPubMedCentralGoogle Scholar
- Kiba, T., Feria-Bourrellier, A.B., Lafouge, F., Lezhneva, L., Boutet-Mercey, S., Orsel, M., Brehaut, V., Miller, A., Daniel-Vedele, F., Sakakibara, H., Krapp, A.: The Arabidopsis nitrate transporter NRT2.4 plays a double role in roots and shoots of nitrogen-straved plants. — Plant Cell 24: 245–258, 2012.CrossRefPubMedPubMedCentralGoogle Scholar
- Sun, J., Chen, S., Dai, S., Wang, R., Li, N., Shen, X., Zhou, X., Lu, C., Zheng, X., Hu, Z., Zhang, Z., Song, J., Xu, Y.: NaCl-induced alternations of cellular and tissue ion fluxes in roots of salt-resistant and salt-sensitive poplar species. — Plant Physiol. 149: 1141–1153, 2009.CrossRefPubMedPubMedCentralGoogle Scholar
- Yuan, L., Loque, D., Kojima, S., Rauch, S., Ishiyama, K., Inoue, E., Takahashi, H., Von Wiren, N.: The organization of high-affinity ammonium uptake in Arabidopsis roots depends on the spatial arrangement and biochemical properties of AMT1-type transporters. — Plant Cell 19: 2636–2652, 2007.CrossRefPubMedPubMedCentralGoogle Scholar