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Biologia Plantarum

, Volume 62, Issue 1, pp 121–128 | Cite as

The effect of boron availability, CO2, and irradiance on relative accumulation of the major boron transport proteins, BOR1 and NIP5;1

  • S. Mishra
  • S. A. Heckathorn
  • J. M. Frantz
  • C. Krause
Original papers
  • 66 Downloads

Abstract

Boron (B) is an essential plant micronutrient. Two major B-transport proteins have been recently identified and partially characterized: BOR1, a high-affinity B efflux transporter involved in xylem loading, and NIP5;1, a plasma-membrane boric-acid channel involved in B uptake. To date, studies of these B transporters have investigated their expression individually (mainly as mRNA), and only in response to variation in B availability (mostly B deficiency); the influence of other factors, such as plant resource status, has not been studied. To address this, we grew geranium (Pelargonium × hortorum cv. Maverick White) plants under ambient or elevated CO2 concentration, different sub-saturating irradiances, and different B availability. For comparison we also grew three other species (Arabidopsis thaliana, Azolla caroliniana, and Hordeum vulgare) under broad range of B supply. Relative accumulation of BOR1 and NIP5;1 proteins were measured using protein-specific antibodies and Western blotting or ELISA. Elevated CO2 significantly increased content of NIP5;1, while increases in irradiance increased BOR1 content, but decreased NIP5;1 content. Across species, content of both transporters often decreased with increasing B availability, but sometimes remained unchanged or even increased, depending on CO2, irradiance, species, or transporter. Content of BOR1 and NIP5;1 was correlated with root proteins, B content, and sugar content (for high CO2 only), as well as B uptake, but CO2 and irradiance often affected these relationships. Thus, relative accumulation of BOR1 and NIP5;1 is influenced not only by B content, as expected, but by other environmental factors as well.

Additional key words

Arabidopsis thaliana Azolla caroliniana B uptake Hordeum vulgare Pelargonium × hortorum photosynthesis 

Abbreviations

BOR1

boron transporter protein 1

CE

carboxylation efficiency of photosynthesis

EDTA

ethylenediaminetetraacetic acid

NIP

NOD26-like intrinsic proteins

PAR

photosynthetically active radiation

pNPP

para-nitrophenylphosphate

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Supplementary material

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Copyright information

© The Institute of Experimental Botany 2018

Authors and Affiliations

  • S. Mishra
    • 1
  • S. A. Heckathorn
    • 1
  • J. M. Frantz
    • 2
  • C. Krause
    • 2
  1. 1.Department of Environmental SciencesUniversity of ToledoToledoUSA
  2. 2.United States Department of Agriculture, Agricultural Research ServiceUniversity of ToledoToledoUSA

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