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Biologia Plantarum

, Volume 62, Issue 1, pp 194–199 | Cite as

Photoperiod and ethylene-dependent expression of gibberellin biosynthesis gene InEKO1 during flower induction of Ipomoea nil

  • K. Marciniak
  • E. Wilmowicz
  • A. Kućko
  • J. Kopcewicz
Brief Communication

Abstract

Ent-kaurene oxidase (EKO) catalyze three sequential oxidations in the early steps of gibberellin biosynthesis pathway. In this research, a cDNA sequence of InEKO1 gene in the model short-day plant Ipomoea nil was identified. Our studies revealed that inductive conditions for flowering caused an increase in the transcriptional activity of the examined gene in the cotyledons–the main organs for the perception of the photoperiodic stimulus. In contrast, in the second half of the 16 h long inductive night and after that, a decreased amount of InEKO1 mRNA in the apexes was detected. What is more, ethylene, the key inhibitor of flower induction in I. nil, elevated the InEKO1 expression exclusively in the cotyledons between 10 and 14 h of the inductive night.

Additional key words

ent-kaurene oxidase flowering phytohormones short-day plant 

Abbreviations

EKO

ent-kaurene oxidase

ET

ethylene

GAs

gibberellins

LD

long day

SD

short day

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Copyright information

© The Institute of Experimental Botany 2018

Authors and Affiliations

  • K. Marciniak
    • 1
    • 2
  • E. Wilmowicz
    • 1
    • 2
  • A. Kućko
    • 1
  • J. Kopcewicz
    • 1
  1. 1.Chair of Plant Physiology and BiotechnologyNicolaus Copernicus UniversityToruńPoland
  2. 2.Centre for Modern Interdisciplinary TechnologiesNicolaus Copernicus UniversityToruńPoland

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