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Hydrogen sulfide is involved in the regulation of ascorbate and glutathione metabolism by jasmonic acid in Arabidopsis thaliana

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  • Published:
Biologia Plantarum

Abstract

This study investigated the role of hydrogen sulfide (H2S) in the regulation of the ascorbate (AsA) and glutathione (GSH) metabolism by jasmonic acid (JA) in the leaves of Arabidopsis thaliana by using H2S scavenger hypotaurine (HT) and H2S synthetic mutant (SALK_041918, designated Atl-cdes). The results showed that JA significantly increased the H2S content, the activities of L-cysteine desulfhydrase (L-CDes), D-cysteine desulfhydrase (D-CDes), ascorbate peroxidase (APX), glutathione reductase (GR), monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), L-galactono-1,4-lactone dehydrogenase (GalLDH) and γ-glutamylcysteine synthetase (γ-ECS), the ratio of AsA to dehydroascorbate (DHA), and decreased the content of malondialdehyde (MDA) and H2O2 in the wild type of A. thaliana, compared to control. The above effects of JA except the increased activities of L-CDes and D-CDes were suppressed by addition of HT. However, JA and HT+JA had no significant effects on the ratio of reduced GSH to oxidized GSH (GSSG) in the wild type of A. thaliana. Application of HT to the control decreased H2S content, AsA/DHA ratio, and activities of APX, GR, DHAR, MDHAR, γ-ECS, and GalLDH, but had no effects on MDA content, activities of L-CDes and D-CDes, and GSH/GSSG ratio. In the H2S synthetic mutant, JA had no obvious effects on above mentioned parameters except the D-CDes activity compared with the control. Our results suggest that JA-induced H2S, which is a signal that leads to the up-regulation of the AsA and GSH metabolism.

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Abbreviations

APX:

ascorbate peroxidase

AsA:

ascorbate

DHAR:

dehydroascorbate reductase

γ-ECS:

γ-glutamylcysteine synthetase

GalLDH:

L-galactono-1,4-lactone dehydrogenase

GR:

glutathione reductase

GSH:

glutathione

HT:

hypotaurine

JA:

jasmonic acid

MDA:

malondiadehyde

MDHAR:

monodehydroascorbate reductase

WT:

wild type

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Authors and Affiliations

  1. Postdoctoral Research Base, Henan Institute of Science and Technology, Xinxiang, 453003, P.R. China

    C. Shan & Y. Zhou

  2. Postdoctoral Research Centre, Henan Agricultural University, Zhengzhou, 450002, P.R. China

    C. Shan & W. Wang

  3. School of Science and Technology, Henan Institute of Science and Technology, Xinxiang, 453003, P.R. China

    C. Shan, T. Wang & Y. Zhou

  4. Collaborative Innovation Center of Modern Biological Breeding, Henan Province, Xinxiang, 453003, P.R. China

    C. Shan, T. Wang & Y. Zhou

Authors
  1. C. Shan
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  2. T. Wang
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  3. Y. Zhou
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  4. W. Wang
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Correspondence to Y. Zhou.

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Acknowledgements: Acknowledgements: This study was funded by the Startup Foundation for Postdoctors of Postdoctoral Research Centre, Henan Agricultural University and Postdoctoral Research Base, Henan Institute of Science and Technology, the Scientific Research Foundation for Postdoctors of Henan Province (2015104), Project of Supporting Young Backbone Teachers of Colleges and Universities in Henan province (2016GGJS-108) and the Innovation Fund of Science and Technology of Henan Institute of Science and Technology in 2015.

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Shan, C., Wang, T., Zhou, Y. et al. Hydrogen sulfide is involved in the regulation of ascorbate and glutathione metabolism by jasmonic acid in Arabidopsis thaliana. Biol Plant 62, 188–193 (2018). https://doi.org/10.1007/s10535-017-0740-9

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  • DOI: https://doi.org/10.1007/s10535-017-0740-9

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