Biologia Plantarum

, Volume 61, Issue 2, pp 349–358 | Cite as

Altered gibberellin content affects growth and development in transgenic tobacco lines overexpressing a wheat gene encoding F-box protein

  • S. Yin
  • S. Zhou
  • X. Kong
  • Y. Han
  • W. WangEmail author
Original Paper


In a previous study, we have identified and characterized gene from wheat (Triticum aestivum L.) encoding F-box protein and named it TaFBA. In this paper, transgenic tobacco (Nicotiana tabacum L.) plants overexpressing TaFBA1 displayed accelerated growth early, but the rate slowed gradually at later stages of growth, and the mature transgenic plants were even shorter in stature and flowered later than did the wild type (WT). Treatment with gibberellin (GA) conferred an accelerated growth rate to the transgenic tobacco plants at later stages, similar to that of WT, whereas growth was inhibited more seriously in WT than in transgenic tobacco when plants were treated with a GA biosynthesis inhibitor. The content of GA in transgenic tobacco plants was higher at early developmental stages, but it was lower at later growth stages than in WT. Some GA biosynthesis genes were down regulated, which was accompanied with elevated expression of a GA catabolism gene. Thus, our results suggest that TaFBA1 is possibly involved in the regulation of plant growth and development, and that it may be related to the production, metabolism, and proper function of GA.

Additional key words

ELISA Nicotiana tabacum paclobutrazol RT-qPCR Triticum aestivum 



enzyme-linked immunosorbent assay






ubiquitin-26S proteasome system




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Supplementary material

10535_2017_707_MOESM1_ESM.pdf (69 kb)
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Copyright information

© The Institute of Experimental Botany 2017

Authors and Affiliations

  1. 1.State Key Laboratory of Crop Biology, Shandong Key Laboratory of Crop Biology, College of Life ScienceShandong Agricultural UniversityTai’an, ShandongP.R. China

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