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Transcriptome-wide identification and expression analyses of ABC transporters in dwarf polish wheat under metal stresses

  • Original Paper
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Biologia Plantarum

Abstract

ABC transporters, which comprise one of the largest protein families, are involved in maintaining osmotic homeostasis, nutrient uptake, pathogen resistance, and metal tolerance. In this study, 30 ABC genes in dwarf polish wheat were characterized and classified into seven subfamilies (ABCA - ABCG). Among them, 24 ABC transporters were newly found in wheat. The expressions of 13 ABC genes in roots and leaves under six metal stresses were also analyzed. All these genes were differentially regulated by Cd (except ABCE2, ABCF4, and ABCF6 in roots), suggesting that these genes participate in Cd transport, sequestration, or uptake. These genes were also differentially regulated by other metals including Cu, Mg, Zn, Fe, and Ni. Results suggest that the expressions of ABC transporters in dwarf polish wheat played important roles in metal transport and detoxification.

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Abbreviations

ABC:

ATP-binding cassette

ACT:

actin

DPW:

dwarf polish wheat

ORFs:

open reading frames

qPCR:

quantitative PCR

WC:

water content

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Authors and Affiliations

  1. Triticeae Research Institute, Sichuan Agricultural University, Sichuan, Wenjiang, 611130, P.R. China

    X. Wang, C. Wang, H. Sheng, Y. Wang, H. Kang, X. Fan, L. Sha, H. Zhang & Y. Zhou

  2. College of Resources, Sichuan Agricultural University, Wenjiang, Sichuan, 611130, P.R. China

    J. Zeng

Authors
  1. X. Wang
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  2. C. Wang
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  3. H. Sheng
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  4. Y. Wang
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  5. J. Zeng
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  6. H. Kang
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  7. X. Fan
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  8. L. Sha
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  9. H. Zhang
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  10. Y. Zhou
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Correspondence to Y. Zhou.

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Acknowledgements: The authors thank the National Natural Science Foundation of China (Nos. 31301349, 31270243 and 31470305), the Bureau of Science and Technology, and the Bureau of Education of Sichuan Province, China.

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Wang, X., Wang, C., Sheng, H. et al. Transcriptome-wide identification and expression analyses of ABC transporters in dwarf polish wheat under metal stresses. Biol Plant 61, 293–304 (2017). https://doi.org/10.1007/s10535-016-0697-0

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  • DOI: https://doi.org/10.1007/s10535-016-0697-0

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