Inhibition of putrescine biosynthesis enhanced salt stress sensitivity and decreased spermidine content in rice seedlings
- 158 Downloads
The effect of polyamine biosynthesis inhibitors on the salt stress response of rice seedlings was investigated. For this, DL-α-difluoromethylarginine (DFMA) and DL-α-difluoromethylornithine (DFMO), two competitive inhibitors of arginine decarboxylase (ADC) and ornithine decarboxylase (ODC), were used. The ADC and ODC are rate-limiting enzymes involved in synthesis of putrescine. The effective quantum yield of photosynthetic energy conversion (ΦPSII) decreased with the salt stress, and this decrease was highly significant in the treatments with DFMA and DFMO. Interestingly, addition of exogenous putrescine reduced the decline of ΦPSII. Putrescine content strongly decreased after one day of the inhibitor treatment. Although the content of spermidine (converted from putrescine) also showed an initial decrease in response to the inhibitors, it recovered to a similar level to that in the control after 3 d of treatment. Under the salt stress, the effect of the inhibitors on the different compounds was similar. Moreover, the addition of exogenous putrescine partially suppressed the decrease in spermidine and spermine content. A positive correlation between the spermidine and spermine content and the ΦPSII was observed. The results suggest that, under salt stress, a decrease in polyamine biosynthesis and/or polyamine content has a strong negative effect on leaves and increases salt stress sensitivity.
Additional key wordsarginine decarboxylase chlorophyll fluorescence ornithine decarboxylase polyamines spermine
effective quantum yield of photosynthetic energy conversion
Unable to display preview. Download preview PDF.
- Alcázar, R., Planas, J., Saxena, T., Zarza, X., Bortolotti, C., Cuevas, J., Bitrián, M., Tiburcio, A.F., Altabella, T.: Putrescine accumulation confers drought tolerance in transgenic Arabidopsis plants over-expressing the homologous Arginine decarboxylase 2 gene. — Plant Physiol. Biochem. 48: 547–552, 2010.CrossRefPubMedGoogle Scholar
- Cuevas, J.C., López-Cobollo, R., Alcázar, R., Zarza, X., Koncz, C., Altabella, T., Salinas, J., Tiburcio, A.F., Ferrando, A.: Putrescine is involved in Arabidopsis freezing tolerance and cold acclimation by regulating abscisic acid levels in response to low temperature. — Plant Physiol. 148: 1094–1105, 2008.CrossRefPubMedPubMedCentralGoogle Scholar
- Flores, H.E.: Changes in polyamine metabolism in response to abiotic stress. - In: Slocum, R.D., Flores, H.E. (ed.): Biochemistry and Physiology of Polyamines in Plants. Pp. 213–228. CRC Press, Boca Raton 1991.Google Scholar
- Fu, X.Z., Xing, F., Wang, N.Q., Peng, L.Z., Chun, C.P., Cao, L., Ling, L.L., Jiang, C.L.: Exogenous spermine pretreatment confers tolerance to combined hightemperature and drought stress in vitro in trifoliate orange seedlings via modulation of antioxidative capacity and expression of stress-related genes. — Biotechnol. Biotech. Eq. 28: 192–198, 2015.CrossRefGoogle Scholar
- Kasugai, S.: Studies on the hydroponic cultures. — J. Sci. Soil Manure 13: 669–822, 1939.Google Scholar
- Marler, T.E., Mickelbart, M.V.: Growth and chlorophyll fluorescence of Spondias purpurea L. as influenced by salinity. — Trop. Agr. 70: 245–247, 1993.Google Scholar
- Yamamoto, A., Sawada, H., Shim, I.S., Usui, K., Fujihara, S.: Effect of salt stress on physiological response and leaf polyamine content in NERICA rice seedlings. — Plant Soil Environ. 57: 571–576, 2011.Google Scholar