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Molecular hydrogen can take part in phytohormone signal pathways in wild rice

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Biologia Plantarum

Abstract

Molecular hydrogen (H2) could be a novel signal in phytohormone signaling pathways in response to biotic and abiotic stresses. Here, we employed two wild rice species (Oryza rufipogon Griff. and O. minuta J. Presl) to test this hypothesis using hydrogen-rich water (HW). The expression differences of phytohormone and hydrogenase genes between conventional rice (Oryza sativa L,) and wild rice were determined by real-time quantitative polymerase chain reaction, and the effects of HW on gene expression of wild rice were detected during three growth stages. Expression of hydrogenase genes, synthesis genes, and receptor genes of salicylic acid (SA), jasmonic acid (JA), and ethylene (ET) signalling pathways was higher in six wild rice types than in conventional rice. Hydrogen-rich water up-regulated expression of two hydrogenase genes, SA, JA, and ET receptor genes and synthesis genes in the seedling stage of wild rice. But this positive regulation by HW was less significant in the vegetative and reproductive stages.

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Abbreviations

ABA:

abscisic acid

AJ:

Anjiashan

CL:

Chaling

CK:

cytokinin

DX:

Dongxiang

ET:

ethylene

GA:

gibberellic acid

GORK:

guard cell outwardly rectifying K+ channel protein

HW:

hydrogen-rich water

JA:

jasmonic acid

LS:

Lingshui

NC:

Nanchang

qPCR:

quatitative polymerase chain reaction

ROS:

reactive oxygen species

SA:

salicylic acid

ST:

Shuitao

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Authors and Affiliations

  1. State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, P.R. China

    F. Liu, J. Li & Y. Liu

Authors
  1. F. Liu
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  2. J. Li
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  3. Y. Liu
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Correspondence to Y. Liu.

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Acknowledgements: This work was financially supported by the National Environmental Protection Public Welfare Science and Technology Research Program of China (grant No. 201309038) and the Special Program for New Transgenic Variety Breeding of the Ministry of Science and Technology, China (grant No. 2012ZX08011002).

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Liu, F., Li, J. & Liu, Y. Molecular hydrogen can take part in phytohormone signal pathways in wild rice. Biol Plant 60, 311–319 (2016). https://doi.org/10.1007/s10535-016-0591-9

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  • DOI: https://doi.org/10.1007/s10535-016-0591-9

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