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Anthocyanin accumulation rate and the biosynthesis related gene expression in Dioscorea alata

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Biologia Plantarum

Abstract

In this study, the anthocyanin content and real-time quantitative expression of the anthocyanin biosynthesis-related genes were investigated in leaves, stems, and tubers of purple yam (Dioscorea alata L.). The anthocyanin content, its accumulation, and the expression of genes encoding phenylalanine ammonia lyase (PAL), flavanone-3-hydroxylase (F3H), anthocyanidin synthase (ANS), and UDP-glycose flavonoid glycosyl transferase (UFGT) were studied. The anthocyanin content in the leaves and stems was high at early stages of growth, but it decreased and remained at a similar level from the 35th day onward. The anthocyanin content in the tubers firstly increased, reached a high peak at the 110th day of growth, after which decreased. Anthocyanin accumulation rates and the expressions of the anthocyanin biosynthesis genes were high at the early stages of growth in the leaves and stems, but in tubers, two peaks were observed: at days 80 and 140 for the gene expression and at days 125 and 170 for the anthocyanin accumulation rate. Thus, there was coordination between the gene expressions and the anthocyanin accumulation rates in the various organs as well as in the entire plants.

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Abbreviations

ANS:

anthocyanidin synthase

CHS:

chalcone synthase

F3H:

flavanone-3-hydroxylase

DFR:

dihydroflavonol-4-reductase

PAL:

phenylalanine ammonia lyase

qRT-PCR:

quantitative real-time PCR

UFGT:

UDP-glycose flavonoid glycosyl transferase

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Authors and Affiliations

  1. Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, P.R. China

    J. M. Yin, R. X. Yan, P. T. Zhang, X. Y. Han & L. Wang

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  1. J. M. Yin
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  2. R. X. Yan
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  3. P. T. Zhang
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  4. X. Y. Han
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  5. L. Wang
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Corresponding author

Correspondence to J. M. Yin.

Additional information

Acknowledgements: This work was supported by the Jiangsu Agricultural Science and Technology Innovation Fund [No.CX(13)5008], China.

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Yin, J.M., Yan, R.X., Zhang, P.T. et al. Anthocyanin accumulation rate and the biosynthesis related gene expression in Dioscorea alata . Biol Plant 59, 325–330 (2015). https://doi.org/10.1007/s10535-015-0502-5

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  • DOI: https://doi.org/10.1007/s10535-015-0502-5

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