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Microsatellite markers designed for tree-fern species Dicksonia sellowiana

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Biologia Plantarum

Abstract

Microsatellite markers were developed for Dicksonia sellowiana (Dicksoniaceae), an overexploited and endangered tree-fern species native to Brazil. From an enriched genomic library, 11 primer pairs were selected and used to characterize 36 D. sellowiana individuals from six Brazilian populations. Eight primer pairs amplified dinucleotide and hexanucleotide repeats with two to ten alleles per locus; three primer pairs were monomorphic. For the set of polymorphic markers, the mean observed and expected heterozygosity ranged from 0.29 to 0.44 and from 0.27 to 0.56, respectively. Eight of the primer pairs were also successfully amplified for Cyathea vestita (Cyatheaceae). These molecular markers can be useful tools for genetic studies aiming to analyze the impact of deforestation and overexploitation on the population structure and genetic diversity of fern species from various botanical families.

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Abbreviations

CITES:

Convention on International Trade in Endangered Species

CTAB:

cetyltrimethylammonium bromide

PCR:

polymerase chain reaction

SSR:

simple sequence repeat

TBE:

Tris/borate/EDTA

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Authors and Affiliations

  1. Avenida Admar Gonzaga 1346, Universidade Federal de Santa Catarina, Florianópolis, 88040-000, Santa Catarina, Brazil

    A. G. Nazareno & M. S. Reis

  2. Empresa Brasileira de Pesquisa Agropecuária, Centro de Pesquisa Agroflorestal da Amazônia Ocidental, Rodovia AM 010, Km 29, CP319, Manaus, 69010-970, Amazonas, Brazil

    P. C. S. Angelo

  3. Programa de Pós-Graduação em Ecologia e Conservação, Laboratório de Ecologia Molecular Vegetal, Centro Politécnico — Universidade Federal do Paraná, CP19031, Curitiba, 81531-990, Paraná, Brazil

    V. C. Muschner & J. Santos

  4. Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis, 88040-970, Santa Catarina, Brazil

    A. D. Schlindwein

Authors
  1. A. G. Nazareno
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  2. P. C. S. Angelo
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  3. V. C. Muschner
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  4. J. Santos
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  5. A. D. Schlindwein
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  6. M. S. Reis
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Corresponding author

Correspondence to A. G. Nazareno.

Additional information

Acknowledgments: The authors gratefully acknowledge the editors and reviewers for their useful comments and suggestions in improving the manuscript. We also thank the Rainforest Research Department, Physiology of Development and the Plant Genetics Laboratory at the Federal University of Santa Catarina (UFSC) for logistical support. A.G. Nazareno was supported by a doctoral fellowship from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). The authors thank Dr. Evelyn R. Nimmo for editing the English of the manuscript.

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Nazareno, A.G., Angelo, P.C.S., Muschner, V.C. et al. Microsatellite markers designed for tree-fern species Dicksonia sellowiana . Biol Plant 57, 563–566 (2013). https://doi.org/10.1007/s10535-013-0311-7

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  • DOI: https://doi.org/10.1007/s10535-013-0311-7

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