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Early transcription of defence-related genes in Platanus × acerifolia leaves following treatment with cerato-platanin

  • Brief Communication
  • Published:
Biologia Plantarum

Abstract

The protein elicitor cerato-platanin (CP) is known to induce defence-related responses in various plants. Some of these responses occur very quickly. In the present work, transcriptional changes caused by CP in leaves from Platanus × acerifolia (Aiton) Willd. were studied. With a cDNA microarray, 131 differentially regulated transcripts were identified as responsive to CP after 24 h of treatment. Eighty-six of these were cold-or ozone-modulated transcripts, thus revealing a significant overlap between genes responsive to CP and to cold/ozone stress. The transcriptional changes caused by CP were compared with the CP-orthologous protein Pop1 in a time-course analysis performed after 3, 6, 12, and 24 h of treatment by real-time RT-PCR on five defence-related genes. Despite some differences, CP and Pop1 were both able to induce early transcriptional changes (WRKY was overexpressed after only 3 h) confirming that pathogenassociated molecular patterns (PAMPs) act very quickly on gene transcription.

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Abbreviations

CP:

cerato-platanin

PAMP:

pathogen-associated molecular pattern

Pop1:

cerato-populin

RT-PCR:

reverse transcriptase-polymerase chain reaction

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Authors and Affiliations

  1. Dipartimento di Scienze delle Produzioni Agroalimentari e dell’Ambiente, Università di Firenze, Firenze, 50134, Italy

    I. Baccelli & A. Scala

  2. Dipartimento di Scienze Biomediche Cliniche e Sperimentali, Università di Firenze, Firenze, 50134, Italy

    L. Pazzagli

  3. Dipartimento di Scienze Agrarie, Alimentari e Agro-ambientali, Università di Pisa, Pisa, 56124, Italy

    R. Bernardi

Authors
  1. I. Baccelli
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  2. A. Scala
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  3. L. Pazzagli
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  4. R. Bernardi
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Corresponding author

Correspondence to I. Baccelli.

Additional information

Acknowledgments: The work was supported by the Ministero Italiano dell’Università e della Ricerca Scientifica, Progetti di Ricerca di Interesse Nazionale 2009.

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Baccelli, I., Scala, A., Pazzagli, L. et al. Early transcription of defence-related genes in Platanus × acerifolia leaves following treatment with cerato-platanin. Biol Plant 57, 571–575 (2013). https://doi.org/10.1007/s10535-013-0308-2

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  • DOI: https://doi.org/10.1007/s10535-013-0308-2

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