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In vitro flowering red miniature rose

Abstract

Using aseptic plantlets obtained from stem node explants of hybrid red miniature rose (Rosa hybrida cv. Fairy Dance), the effects of shoot physiological status, medium ingredients, and culture thermoperiod on in vitro flowering were evaluated. Shoot height, subculture media for shoot multiplication, sucrose concentration, plant growth regulators (PGRs), mineral substances in media, and thermoperiod had a significant effect on the percentage of in vitro flowering. Shoots 3 ± 0.2 or 2 ± 0.2 cm in height cultured on Murashige and Skoog (MS) medium containing 2.0 mg dm−3 6-benzyladenine (BA), 0.2 mg dm−3 α-naphthaleneacetic acid (NAA), and 20 g dm−3 sucrose were more suitable for in vitro flowering than shoots 4 ± 0.2, or 5 ± 0.2 cm in height. The most suitable sucrose concentration for in vitro flowering was 50 g dm−3 and the most suitable PGRs were a combination of 3.0 mg dm−3 BA and 0.1 mg dm−3 NAA. Increasing the potassium nitrate to ammonium nitrate ratio or increasing the phosphate concentration in MS medium had a positive effect on in vitro flowering. The percentage of in vitro flowering was significantly higher at day/night temperature of 28/20 °C than at other constant temperatures. The percentage of in vitro flowering shoots reached 68.33 % despite the occurrence of abnormal flowers and some unique developmental patterns. It makes miniature rose a potentially new in vitro experimental platform for research on the molecular mechanisms of flowering ornamental plants.

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Abbreviations

BA:

6-benzyladenine

DMRT:

Duncan’s multiple range test

IBA:

indole-3-butyric acid

KIN:

kinetin

MS:

Murashige and Skoog medium

NAA:

α-naphthaleneacetic acid

PGR:

plant growth regulator

PPFD:

photosynthetic photon flux density

TDZ:

thidiazuron

ZT:

zeatin

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Correspondence to J. Duan.

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Acknowledgements: This study was supported by the Guangdong Key Technology Research and Development Program (2011B020304004; 2010B060200037) and by a fund to the Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences (211026). The first two authors contributed equally to this work.

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Zeng, S., Liang, S., Zhang, Y.Y. et al. In vitro flowering red miniature rose. Biol Plant 57, 401–409 (2013). https://doi.org/10.1007/s10535-013-0306-4

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Additional key words

  • abnormal flower
  • auxins
  • cytokinins
  • minerals
  • Rosa hybrida
  • thermoperiod