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Cryptic homoeology analysis in species and hybrids of genus Zea

  • Published:
Biologia Plantarum

Abstract

Cryptic intergenomic pairing of genus Zea was induced by the use of a diluted colchicine solution in order to elucidate the phylogenetic relations and differentiation of the homoeologous genomes. Results indicate that in species and hybrids with 2n = 20, there was chromosome pairing between the homoeologous A and B genomes with a maximum of 5IV, with the exception of Zea diploperennis and their interspecific hybrids where cryptic homoeologous chromosome pairing was not induced. In almost all 2n = 30 hybrids, observed cryptic pairing increased to a maximum of 10III although Z. mays × Z. mays with 2n = 30 did not show significant differences between treated and untreated materials. Pairing was also observed in species and hybrids with 2n = 40, in which a maximum of 10IV was observed, with the exception of Z. mays with 2n = 40 where treated and untreated cells did not differ significantly.

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Abbreviations

A:

subgenome A Zea

B:

subgenome B Zea

Bp1:

subgenome Bp1 Zea perennis

Bp2:

subgenome Bp2 Zea perennis

2,4-D:

2,4 dichlorophenoxyacetic

I:

chromosome monovalent

II:

chromosome bivalent

III:

chromosome trivalent

IV:

chromosome quadrivalent

pam1 :

gene plural abnormalities of meiosis

Ph :

gene pairing homoeologous

Ph1 :

pairing homoeologous 1

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Authors and Affiliations

  1. Instituto Fitotécnico de Santa Catalina, Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de la Plata, Garibaldi, 3400, Llavallol, Buenos Aires, Argentina

    M. del C. Molina & S. Staltari

  2. Consejo Nacional de Investigaciones Científicas y Técnicas, Rivadavia, 1917, Ciudad Autónoma de Buenos Aires, Argentina

    M. del C. Molina

  3. Facultad de Ciencias Agrarias, Universidad Nacional de Lomas de Zamora, Ruta 4 Km. 2, Buenos Aires, Argentina

    C. G. López & S. E. Chorzempa

  4. Departamento de Biotecnología, Universidad Politécnica de Valencia and Instituto de Biología Molecular y Celular de Plantas, Valencia, Spain

    V. Moreno Ferrero

Authors
  1. M. del C. Molina
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  2. C. G. López
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  3. S. Staltari
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  4. S. E. Chorzempa
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  5. V. Moreno Ferrero
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Correspondence to M. del C. Molina.

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Acknowedgments: This research was supported by the Universidad Nacional de la Plata, Universidad Nacional de Lomas de Zamora and CONICET.

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Molina, M.d.C., López, C.G., Staltari, S. et al. Cryptic homoeology analysis in species and hybrids of genus Zea . Biol Plant 57, 449–456 (2013). https://doi.org/10.1007/s10535-012-0299-4

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  • DOI: https://doi.org/10.1007/s10535-012-0299-4

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