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GmPOI gene encoding a Pollen_Ole_e_I conserved domain is involved in response of soybean to various stresses

  • Published:
Biologia Plantarum

Abstract

In the previous research, a novel gene GmPOI (GenBank acc. No. HM235775) encoding a Pollen_Ole_e_I conserved domain was identified in roots of soybean drought resistant cv. Jindou 23. In the present study, GmPOI was cloned and functionally characterized. Real-time quantitative PCR indicated that the expression of GmPOI was induced by drought, cold, salt and abscisic acid in wild-type soybean. The soybean plants overexpressing GmPOI showed higher tolerance to drought stress than wild types. We concluded that GmPOI is probably a novel gene that is involved in the response to various stresses in soybean.

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Abbreviations

ABA:

abscisic acid

EST:

expressed sequence tag

ROS:

reactive oxygen species

RT-PCR:

reverse transcription polymerase chain reaction

SOD:

superoxide dismutase

WT:

wild type

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Authors and Affiliations

  1. College of Crop Protection and Agronomy, Qingdao Agricultural University, Qingdao, 266109, P.R. China

    W. W. Song, F. M. Duan, Q. Lin & H. X. Zhou

  2. College of Agronomy, Northeast Agricultural University, Harbin, 150030, P.R. China

    W. B. Li & J. A. Wang

  3. The Crop Research and Breeding Center of Heilongjiang Land-Reclamation, Harbin, 150090, P.R. China

    X. Han

Authors
  1. W. W. Song
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  2. F. M. Duan
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  3. W. B. Li
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  4. Q. Lin
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  5. H. X. Zhou
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  6. X. Han
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  7. J. A. Wang
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Correspondence to J. A. Wang.

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Acknowledgements: This work was funded by the Taishan Mountain Scholar Constructive Engineering Foundation of Shandong and financially supported by the Key Project of Transgenic Organisms in China (2009ZX08009-089B). The first two authors contributed equally to the work.

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Song, W.W., Duan, F.M., Li, W.B. et al. GmPOI gene encoding a Pollen_Ole_e_I conserved domain is involved in response of soybean to various stresses. Biol Plant 57, 85–90 (2013). https://doi.org/10.1007/s10535-012-0258-0

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  • DOI: https://doi.org/10.1007/s10535-012-0258-0

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