Biologia Plantarum

, Volume 57, Issue 1, pp 174–178 | Cite as

The role of cytokinins during micropropagation of wych elm

  • J. Malá
  • P. Máchová
  • H. Cvrčková
  • M. Karady
  • O. Novák
  • J. Mikulík
  • J. Dostál
  • M. Strnad
  • K. DoležalEmail author
Brief Communication


We have compared the influence of two aromatic cytokinin derivatives, N 6-benzyladenine (BA) and meta-topolin (mT), on the in vitro multiplication and senescence of wych elm (Ulmus glabra Huds.). After 3 months of cultivation, the micropropagation rate was higher (approx. six times more shoots developed) on Murashige and Skoog (MS) medium supplemented with mT than on MS supplemented with BA. Quantification of 50 endogenous cytokinins, using a recently developed UPLC-ESI(+)-MS/MS method, showed significant differences in the cytokinin metabolites (especially different glucosides) in explants cultivated on media supplemented with BA or mT. Differences in efficiency of photosystem II was also detected.

Additional key words

benzylaminopurine chlorophyll fluorescence meta-topolin organogenesis rooting senescence 



N 6-benzyladenine






variable to maximum chlorophyll fluorecence ratio


9-β-D-glucopyranosyl derivative


immunoaffinity chromatography


indole-3-butyric acid


N 6-isopentenyladenine


5′-monophosphate derivative


liquid chromatography combined with mass spectrometry


Murashige and Skoog medium


meta-topolin [6-(3-hydroxybenzylamino)purine]


O-β-D-glucopyranosyl derivative




photosystem II


9-β-D-ribofuranosyl derivative




ultra performance liquid chromatography combined with positive electrospray mass spectrometry


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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • J. Malá
    • 1
  • P. Máchová
    • 1
  • H. Cvrčková
    • 1
  • M. Karady
    • 2
  • O. Novák
    • 2
  • J. Mikulík
    • 2
  • J. Dostál
    • 1
  • M. Strnad
    • 2
    • 3
  • K. Doležal
    • 2
    • 3
    Email author
  1. 1.Forestry and Game Management Research InstituteJílovištěCzech Republic
  2. 2.Laboratory of Growth RegulatorsPalacký University and Institute of Experimental Botany AS CROlomouc-HoliceCzech Republic
  3. 3.Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of SciencePalacký UniversityOlomoucCzech Republic

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