Molecular cloning and characterization of a chlorophyll degradation regulatory gene from bamboo
- 378 Downloads
Leaf senescence constituted the final stage of leaf development and it is always accompanied by the leaf yellowing. The non-yellowing gene (NYE1), initially identified from Arabidopsis in our laboratory, is a key regulatory gene responsible for chlorophyll degradation during senescence. In this study, an orthologue of AtNYE1 was isolated from the bamboo (Bambusa emeiensis cv. Viridiflavus) and tentatively named BeNYE1. The full length sequence of 1 386 bp contains an open reading frame of 801 bp. The protein encoded by BeNYE1 consists of 266 amino acids. Sequence analysis revealed that BeNYE1 had high similarity with other NYE/SGR proteins from various monocotyledon species. BeNYE1 was strongly induced by natural senescence and dark-induced senescence in bamboo. Driven by a 1.5 kb upstream fragment of AtNYE1, BeNYE1 could rescue the stay-green phenotype of nye1-1. The constitutive over-expression of BeNYE1 could accelerate the chlorophyll degradation. These results indicated that BeNYE1 might play an important role in the regulation of chlorophyll degradation during leaf senescence in bamboo.
Additional key wordsBambusa emeiensis leaf senescence NYE1 stay-green phenotype
open reading frame
rapid amplification of cDNA ends
Unable to display preview. Download preview PDF.
- Alos, E., Roca, M., Iglesias, D.J., Minguez-Mosquera, M.I., Damasceno, C.M., Thannhauser, T.W., Rose, J.K., Talon, M., Cercos, M.: An evaluation of the basis and consequences of a stay-green mutation in the navel negra citrus mutant using transcriptomic and proteomic profiling and metabolite analysis. — Plant Physiol. 147:1300–1315, 2008.PubMedCrossRefGoogle Scholar
- Armstead, I., Donnison, I., Aubry, S., Harper, J., Hortensteiner, S., James, C., Mani, J., Moffet, M., Ougham, H., Roberts, L., Thomas, A., Weeden, N., Thomas, H., King, I.: From crop to model to crop: identifying the genetic basis of the stay-green mutation in the Lolium/Festuca forage and amenity grasses. — New Phytol. 172: 592–597, 2006.PubMedCrossRefGoogle Scholar