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Variability for resistance to Fusarium solani culture filtrate and fusaric acid among somaclones in pea

Abstract

Pea (Pisum sativum L.) somaclones of cultivars Adept, Komet and Bohatýr were obtained after selection in vitro with Fusarium solani filtrate and fusaric acid (FA). R2 regenerants were analysed by random amplification of polymorphic DNA (RAPD; OPAB4, P-14, UBC-556) and inter-retrotransposon amplification polymorphism (IRAP; Ogre) markers. Marker UBC-556 showed different banding patterns for each cultivar, but without specific bands for selected and control plants. Markers OPAB4, P14 and Ogre were useful for clear discrimination between selected and non-selected variants of all three cultivars. Flow cytometry analysis proved the same genome size of selected and non-selected pea lines. Therefore in vitro selection by pathogen derived agents could be the efficient method for obtaining of pea somaclones with increased resistance to F. solani.

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Abbreviations

BAP:

benzylaminopurine

FA:

fusaric acid

IRAP:

inter-retrotransposon amplification polymorphism

ISSR:

intersimple sequence repeat

NAA:

naphthaleneacetic acid

RAPD:

random amplification of polymorphic DNA

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Correspondence to A. Lebeda.

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Acknowledgements: This research was supported by grants of Czech Ministry of Education, Youth and Sports MSM 6198959215, MSM 2678424601 and National Agency for Agricultural Research QG60099. The authors thank to Dr. Petr Smýkal for providing primers for RAPD and IRAP analyses.

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Horáček, J., Švábová, L., Šarhanová, P. et al. Variability for resistance to Fusarium solani culture filtrate and fusaric acid among somaclones in pea. Biol Plant 57, 133–138 (2013). https://doi.org/10.1007/s10535-012-0131-1

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Additional key words

  • in vitro culture
  • fusarium wilt
  • IRAP
  • molecular markers
  • Pisum sativum
  • RAPD
  • selection agent