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Effect of 24-epibrassinolide on drought stress-induced changes in Chorispora bungeana

Abstract

Brassinosteroids (BRs) have been proposed to increase the resistance of plants to drought stress. The effect of foliar application of 0.1 μM 24-epibrassinolide (EBR) on chlorophyll (Chl) content, photosystem 2 (PS 2) photochemistry, membrane permeability, lipid peroxidation, relative water content (RWC), proline content, and the antioxidant system in drought-stressed Chorispora bungeana plants was investigated. The results showed that polyethylene glycol (PEG) induced water stress decreased RWC, Chl content and variable to maximum Chl fluorescence ratio (Fv/Fm) less in plants pretreated with EBR than in non-pretreated plants. In addition, lipid peroxidation, measured in terms of malondialdehyde content, membrane permeability and proline content in drought-stressed plants were less increased in EBR pretreated plants, while antioxidative enzyme activities and reduced ascorbate and glutathione contents were more increased in EBR pretreated than in non-pretreated plants. These results suggested that EBR could improve plant growth under drought stress

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Abbreviations

APX:

ascorbate peroxidase

AsA:

reduced ascorbate

BRs:

brassinosteroids

CAT:

catalase

Chl:

chlorophyll

EBR:

24-epibrassinolide

EDTA:

ethylenediaminetetraacetic acid

EL:

electrolyte leakage

F0, Fm :

minimum and maximum fluorescence in dark-adapted leaves

Fv/Fm :

variable to maximum fluorescence ratio

GR:

glutathione reductase

GSH:

reduced glutathione

MDA:

malondialdehyde

PEG:

polyethylene glycol

PS 2:

photosystem 2

ROS:

reactive oxygen species

RWC:

relative water content

SOD:

superoxide dismutase

ΦPS2 :

the quantum yield of PS 2

References

  • Abassi, N.A., Kushad, M.M., Endress, A.G.: Active oxygen scavenging enzymes activities in developing apple flowers and fruits. — Sci. Hort. 74: 183–194, 1998.

    Article  Google Scholar 

  • Ábrahám, E., Rigó, G., Székely, G., Nagy, R., Koncz, C., Szabados, L.: Light-dependent induction of proline biosynthesis by abscisic acid and salt stress is inhibited by brassinosteroid in Arabidopsis. — Plant mol. Biol. 51: 363–372, 2003.

    PubMed  Article  Google Scholar 

  • Ali, B., Hayat, S., Ahmad, A.: 28-Homobrassinolide ameliorates the saline stress in chickpea (Cicer arietinum L). — Environ. exp. Bot. 59: 217–223, 2007.

    Article  CAS  Google Scholar 

  • Bajji, M., Lutts, S., Kinet, J.M.: Water deficit effect on solution contribution to osmotic adjustment as a function of leaf ageing in three durum wheat (Triticum durum Desf.) cultivars performing differently in arid conditions. — Plant Sci. 160: 669–681, 2001.

    PubMed  Article  CAS  Google Scholar 

  • Bates, L.S., Waldren, R.P., Teare, I.D.: Rapid determination of free proline for water-stress studies. — Plant Soil 39: 205–207, 1973.

    Article  CAS  Google Scholar 

  • Blum, A., Ebercon, A.: Cell membrane stability as a measure of drought and heat tolerance in wheat. — Crop Sci. 21: 43–47, 1981.

    Article  Google Scholar 

  • Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. — Anal. Biochem. 72: 248–254, 1976.

    PubMed  Article  CAS  Google Scholar 

  • Cai, Y.F., Zhang, S.B., Hu, H., Li, S.Y.: Photosynthetic performance and acclimation of Incarvillea delavayi to water stress. — Biol. Plant. 54: 89–96, 2010.

    Article  CAS  Google Scholar 

  • Clouse, S.D., Sasse, J.M.: Brassinosteroids: essential regulators of plant growth and development. — Annu. Rev. Plant Physiol. Plant mol. Biol. 49: 427–451, 1998.

    PubMed  Article  CAS  Google Scholar 

  • Correia, M.J., Osório, M.L., Osório, J., Barrote, I., Martins, M., David, M.M.: Influence of transient shade periods on the effects of drought on photosynthesis, carbohydrate accumulation and lipid peroxidation in sunflower leaves. — Environ. exp. Bot. 58: 75–84, 2006.

    Article  CAS  Google Scholar 

  • Fariduddin, Q., Yusuf, M., Hayat, S., Ahmad, A.: Effect of 28- homobrassinolide on antioxidant capacity and photosynthesis in Brassica juncea plants exposed to different levels of copper. — Environ. exp. Bot. 66: 418–424, 2009.

    Article  CAS  Google Scholar 

  • Foyer, C.H., Halliwell, B.: The presence of glutathione and glutathione reductase in chloroplasts: a proposed role in ascorbic acid metabolism. — Planta 133: 21–25, 1976.

    Article  Google Scholar 

  • Foyer, C.H., Lelandais, M., Kunert, K.J.: Photooxidative stress in plants. — Physiol. Plant. 92: 696–717, 1994.

    Article  CAS  Google Scholar 

  • Foyer, C.H., Noctor, G.: Oxidant and antioxidant signalling in plants: a re-evaluation of the concept of oxidative stress in a physiological context. — Plant Cell Environ. 28: 1056–1071, 2005.

    Article  CAS  Google Scholar 

  • Genty, B., Briantais, J.M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. — Biochim. biophys. Acta 990: 87–92, 1989.

    Article  CAS  Google Scholar 

  • Griffith, O.W.: Determination of glutathione and glutathione disulphide using glutathione reductase and 2-vinylpyridine. — Anal. Biochem. 106: 207–212, 1980.

    PubMed  Article  CAS  Google Scholar 

  • Hodges, M.D., Delong, J.M., Forney, C.F., Prange, R.K.: Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. — Planta 207: 604–611, 1999.

    Article  CAS  Google Scholar 

  • Jain, M., Mathur, G., Koul, S., Sarin, N.B.: Ameliorative effects of proline on salt stress-induced lipid peroxidation in cell lines of groundnut (Arachis hypogea L.). — Plant Cell Rep. 20: 463–468, 2001.

    Article  CAS  Google Scholar 

  • Law, M.Y., Charles, S.A., Halliwell, B.: Glutathione and ascorbic acid in spinach (Spinacia oleracea) chloroplasts. The effect of hydrogen peroxide and paraquat. — Biochem. J. 210: 899–903, 1983.

    PubMed  CAS  Google Scholar 

  • Lee, B.R., Li, L.S., Jung, W.J., Jin, Y.L., Avice, J.C., Ourry, A., Kim, T.H.: Water deficit-induced oxidative stress and the activation of antioxidant enzymes in white clover leaves. — Biol. Plant. 53: 505–510, 2009.

    Article  Google Scholar 

  • Lichtenthaler, H.K.: Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. — Methods Enzymol. 148: 350–382, 1987.

    Article  CAS  Google Scholar 

  • Lin, K.H., Pu, S.F.: Tissue- and genotype-specific ascorbate peroxidase expression in sweet potato in response to salt stress. — Biol. Plant. 54: 664–670, 2010.

    Article  CAS  Google Scholar 

  • Maxwell, K., Johnson, G.N.: Chlorophyll fluorescence - a practical guide. — J. exp. Bot. 51: 659–668, 2000.

    PubMed  Article  CAS  Google Scholar 

  • Moran, J.F., Becana, M., Iturbe-Ormaetxe, I., Frechilla, S., Klucas, R.V., Aparicio-Tejo, P.: Drought induces oxidative stress in pea plants. — Planta 194: 346–352, 1994.

    Article  CAS  Google Scholar 

  • Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. — Physiol. Plant. 15: 473–497, 1962.

    Article  CAS  Google Scholar 

  • Nakano, Y., Asada, K.: Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. — Plant Cell Physiol. 22: 867–880, 1981.

    CAS  Google Scholar 

  • Ogweno, J.O., Song, X.S., Shi, K., Hu, W.H., Yu, J.Q., Nogues, S.: Brassinosteroids alleviate heat-induced inhibition of photosynthesis by increasing carboxylation efficiency and enhancing antioxidant systems in Lycopersicon esculentum. — J. Plant Growth Regul. 27: 49–57, 2008.

    Article  CAS  Google Scholar 

  • Reddy, M.P., Vora, A.B.: Changes in pigment composition, Hill reaction activity and saccharide metabolism in bajra (Pennisetum typhoides S&H) leaves under NaCl salinity. — Photosynthetica 20: 50–55, 1986.

    CAS  Google Scholar 

  • Santos, M.G., Ribeiro, R.V., Machado, E.C., Pimentel, C.: Photosynthetic parameters and leaf water potential of five common bean genotypes under mild water deficit. — Biol. Plant. 53: 229–236, 2009.

    Article  CAS  Google Scholar 

  • Stewart, R.R.C., Bewley, J.D.: Lipid peroxidation associated with accelerated aging of soybean axes. — Plant Physiol. 65: 245–248, 1980.

    PubMed  Article  CAS  Google Scholar 

  • Stobart, A.K., Griffits, W.T., Bukhari, I., Sherwood, R.P.: The effect of Cd2+ on the biosynthesis of chlorophyll in leaves of barley. — Physiol. Plant. 63: 293–298, 1985.

    Article  CAS  Google Scholar 

  • Veljovic-Jovanovic, S., Kukavica, B., Stevanovic, B., Navari-Izzo, F.: Senescence and drought-related changes in peroxidase and superoxide dismutase isoforms in leaves of Ramonda serbica. — J. exp. Bot. 57: 1759–1768, 2006.

    PubMed  Article  CAS  Google Scholar 

  • Xia, X.J., Wang, Y.J., Zhou, Y.H., Tao, Y., Mao, W.H., Shi, K., Asami, T., Chen, Z.X., Yu, J.Q.: Reactive oxygen species are involved in brassinosteroid-induced stress tolerance in cucumber. — Plant Physiol. 150: 801–814, 2009.

    PubMed  Article  CAS  Google Scholar 

  • Yu, J.Q., Huang, L.F., Hu, W.H., Zhou, Y.H., Mao, W.H., Ye, S.F., Nogues, S.: A role of brassinosteroids in the regulation of photosynthesis in Cucumis sativus. — J. exp. Bot. 55: 1135–1143, 2004.

    PubMed  Article  CAS  Google Scholar 

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (grant No. 30900172), the National Outstanding Youth Foundation of China (No. 30625008), and the Major Project of Cultivating New Varieties of Transgenic Organisms (2009ZX08009-029B, 2008ZX08009-003)

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Correspondence to H. Zhang.

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Li, Y.H., Liu, Y.J., Xu, X.L. et al. Effect of 24-epibrassinolide on drought stress-induced changes in Chorispora bungeana . Biol Plant 56, 192–196 (2012). https://doi.org/10.1007/s10535-012-0041-2

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  • DOI: https://doi.org/10.1007/s10535-012-0041-2

Additional key words

  • antioxidative enzymes
  • brassinosteroids
  • chlorophyll
  • lipid peroxidation
  • membrane permeability
  • photosystem
  • proline
  • reactive oxygen species