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Molecular cloning and characterization of nucleoside diphosphate kinase 1 cDNA in tea

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Biologia Plantarum

Abstract

Nucleoside diphosphate kinase (NDPK) operates in the homeostasis of cellular nucleoside triphosphate (NTP) pools and the cytosolic NDPK1 is the main NDPK isoform in plants, accounting for more than 70 % of total NDPK activity in plant. A full length cDNA (697 bp), designated as CsNDPK1 was cloned from Camellia sinensis (L.) O. Kuntze leaves. Sequence analysis of CsNDPK1 shows several motifs, binding and catalytic sites which are highly conserved among other NDPKs. Southern blot analysis revealed that tea genome has two copies of CsNDPK1. Transcription pattern analysis indicated that CsNDPK1 is expressed in all tissues examined, but expressed more in buds than in other organs.

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Abbreviations

DTT:

dithiothreitol

EST:

expressed sequence tag

NDPK:

nucleoside diphosphate kinase

NTP:

nucleoside triphosphate

PCR:

polymerase chain reaction

RACE:

rapid amplification of cDNA ends

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Acknowledgements

Authors are thankful to Dr. P. Mohan Kumar, Director and Dr. N. Muraleedharan, Adviser of UPASI Tea Research Foundation for their encouragement and support during the course of study. Financial assistance from NTRF, Tea Board, Kolkata is also gratefully acknowledged.

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Authors and Affiliations

  1. UPASI-Tea Research Foundation, Nirar Dam B.P.O, Valparai, 642127, Tamil Nadu, India

    G. R. Prabu, K. Thirugnanasambantham, A. K. A. Mandal & A. Saravanan

  2. Department of Biotechnology, Karpagam University, Coimbatore, 641021, Tamil Nadu, India

    G. R. Prabu

  3. School of Bio Sciences and Technology, VIT University, Vellore, 632014, Tamil Nadu, India

    A. K. A. Mandal

Authors
  1. G. R. Prabu
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  2. K. Thirugnanasambantham
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  3. A. K. A. Mandal
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  4. A. Saravanan
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Correspondence to A. K. A. Mandal.

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Prabu, G.R., Thirugnanasambantham, K., Mandal, A.K.A. et al. Molecular cloning and characterization of nucleoside diphosphate kinase 1 cDNA in tea. Biol Plant 56, 140–144 (2012). https://doi.org/10.1007/s10535-012-0030-5

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  • DOI: https://doi.org/10.1007/s10535-012-0030-5

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