Abstract
In this study, we report a function of myo-inositol-O-methyltransferase (Imt1) in response to low temperature stress using transgenic Arabidopsis thaliana. Imt1 gene was constructed identical to the Imt1 gene from a halophyte Mesembryanthemum crystallinum. After cold stress, the Imt1 transgenic plants exhibited stronger growth than the wild type plants. The elevated cold tolerance of the Imt1 over-expressing plants was confirmed by the lower electrolyte leakage and accumulation of malondialdehyde, but higher proline and soluble sugar contents in transgenic than wild type plants.
This is a preview of subscription content, log in via an institution to check access.
Abbreviations
- Imt1:
-
myo-inositol O-methyltransferase
- MDA:
-
malondialdehyde
- nt:
-
nucleotide
- OE-PCR:
-
overlap extension polymerase chain reaction
- PTDS:
-
PCR-based two-step DNA synthesis
References
Alferez, F., Pozo, L., Burns, J.K.: Physiological changes associated with senescence and abscission in mature citrus fruit induced by 5-chloro-3-methyl-4-nitro-1H-pyrazole and ethephon application. — Physiol. Plant. 127: 66–73, 2006.
Bates, L.S., Waldren, R.P., Teare, I.D.: Rapid determination of free proline in water-stress studies. — Plant Soil 39: 205–207, 1973.
Cakmak, I., Horst, W.J.: Effect of aluminium on lipid peroxidation, superoxide dismutase, catalase, and peroxidase activities in root tips of soybean (Glycine max). — Physiol. Plant. 83: 463–468, 1991.
Clough, S.J., Bent, A.F.: Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. — Plant J. 16: 735–743, 1998.
James, G.W., Wang, H.Y., Daniel, J.G.: Expression of a synthetic antifreeze protein in potato reduces electrolyte release at freezing temperatures. — Plant mol. Biol. 35: 323–330, 1997.
Oberschall, A., Deak, M., Torok, K., Sass, L., Vass, I., Kovacs, I., Feher, A., Dudits, D., Horvath, G.V.: A novel aldose/ aldehyde reductase protects transgenic plants against lipid peroxidation under chemical and drought stresses. — Plant J. 24: 437–446, 2000.
Peng, R.H., Huang, X.M., Li, X., Sun, A.J., Yao, Q.H., Peng, Y.L.: Construction of a plant binary expression vector containing intron-kanamycin gene and transformation in Nicotiana tabacum. — Acta phytophysiol. sin. 27: 55–60, 2001.
Peng, R.H., Xiong, A.S., Yao, Q.H.: A direct and efficient PAGE-mediated overlap extension method for gene multiple-site mutagenesis. — Appl. Microbiol. Biotechnol. 73: 234–240, 2006.
Rammesmayer, G., Pichorner, H.D., Adams, P., Jensen, R.G., Bohnert, H.J.: Characterization of IMT1, myo-Inositol Omethyltransferase, from Mesembryanthemum crystallinum. — Arch. Biochem. Biophys. 322: 183–188, 1995.
Sanchez, F.J., Manzaranes, M., De Andres, E.F., Tenorio, J.L., Ayerbe, L.: Turgor maintenance, osmotic adjustment and soluble sugar and proline accumulation in 49 pea cultivars in response to water stress. — Field Crops Res. 59: 225–235, 1998.
Seki, M., Narusaka, M., Ishida, J., Nanjo, T., Fujita, M., Oono, Y., Kamiya, A., Nakajima, M, Enju, A., Sakurai, T., Satou, M, Akiyama, K., Taji, T., Yamaguchi-Shinozaki, K., Carninci, P., Kawai, J., Hayashizaki, Y., Shinozaki, K.: Monitoring the expression profiles of 7000 Arabidopsis genes under drought, cold and high-salinity stresses using a full-length cDNA microarray. — Plant J. 31: 279–292, 2002.
Sheveleva, E., Chmara, W., Bohnert, H.J., Jensen, R.G..: Increased salt and drought tolerance by D-ononitol production in transgenic Nicotiana tabacum L. — Plant Physiol. 115: 1211–1219, 1997.
Sunkar, P., Bartels, D., Kirch, H.H.: Overexpression of a stressinducible aldehyde dehydrogenase gene from Arabidopsis thaliana in transgenic plants improves stress tolerance. — Plant J. 35: 452–464, 2003.
Thomashow, M.F.: Role of cold-responsive genes in plant freezing tolerance. — Plant Physiol. 118: 1–7, 1998.
Thomashow, M.F.: Plant cold acclimation, freezing tolerance genes and regulatory mechanisms. — Annu. Rev. Plant. Physiol. Plant. mol. Biol. 50: 571–599, 1999.
Tsutsui, T., Kato, W., Asada, Y., Sako, K., Sako, K., Sonoda, Y., Kidokoro, S., Yamaguchi-Shinozaki, K., Tamaoki, M., Arakawa, K., Ichikawa, T., Nakazawa, M., Seki, M., Shinozaki, K., Matsui, M., Ikeda, A., Yamaguchi, J.: DEAR1, a transcriptional repressor of DREB protein that mediates plant defense and freezing stress responses in Arabidopsis. — J. Plant Res. 122: 633–643, 2009.
Vernon, D.M., Bohnert, H.J.: A novel methyl transferase induced by osmotic stress in the facultative halophyte Mesembryanthemum cytstallinum. — The EMBO J. 11: 2077–2085, 1992.
Watanabe, S., Kojima, K., Ide, Y., Sasaki, S.: Effects of saline and osmotic stress on proline and sugar accumulation in Populus euphratica in vitro. — Plant Cell Tissue Organ Cult. 63: 199–206, 2000.
Xiong, A.S., Yao, Q.H., Peng, R.H., Li, X., Fan, H.Q., Cheng, Z.M., Li, Y.: A simple, rapid, high-fidelity and costeffective PCR-based two-step DNA synthesis method for long gene sequence. — Nucl. Acids Res. 32 (Suppl.): e98, 2004.
Xiong, A.S., Yao, Q.H., Peng, R.H., Duan, H., Li, X., Fan, H.Q., Cheng, Z.M., Li, Y.: PCR-based accurate synthesis of long DNA sequences. — Nat. Protocols 1: 791–797, 2006.
Xue, Y., Peng, R., Xiong, A., Li, X., Zha, D., Yao, Q.: Overexpression of heat shock protein gene hsp26 in Arabidopsis thaliana enhances heat tolerance. — Biol. Plant. 54: 105–111, 2010.
Yemm, E.W., Willis, A.J.: The estimation of carbohydrates in plant extracts by anthrone. — Biochem. J. 57: 508–514, 1954.
Acknowledgments
The research was supported by 863 Program No. 2006AA10Z117 and Shanghai Basic Research Project No. 08JC1418000.
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
Zhu, B., Peng, R.H., Xiong, A.S. et al. Transformation with a gene for myo-inositol O-methyltransferase enhances the cold tolerance of Arabidopsis thaliana . Biol Plant 56, 135–139 (2012). https://doi.org/10.1007/s10535-012-0029-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10535-012-0029-y