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Aluminum-induced changes in reactive oxygen species accumulation, lipid peroxidation and antioxidant capacity in wheat root tips

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Biologia Plantarum


The present study investigated the effects of aluminum on lipid peroxidation, accumulation of reactive oxygen species and antioxidative defense systems in root tips of wheat (Triticum aestivum L.) seedlings. Exposure to 30 μM Al increased contents of malondialdehyde, H2O2, suproxide radical and Evans blue uptake in both genotypes, with increases being greater in Al-sensitive genotype Yangmai-5 than in Al-tolerant genotype Jian-864. In addition, Al treatment increased the activity of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), glutathione reductase (GR) and glutathione peroxidase (GPX), as well as the contents of ascorbate (AsA) and glutathione (GSH) in both genotypes. The increased activities SOD and POD were greater in Yangmai-5 than in Jian-864, whereas the opposite was true for the activities of CAT, APX, MDHAR, GR and GPX and the contents of AsA and GSH. Consequently, the antioxidant capacity in terms of 2,2-diphenyl-1-picrylhydrazyl (DPPH)-radical scavenging activity and ferric reducing/antioxidant power (FRAP) was greater in Jian-864 than in Yangmai-5.

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ascorbate peroxidase






dehydroascorbate reductase




ferric reducing/antioxidant power


glutathione peroxidase


glutathione reductase


reduced glutathione


oxidized glutathione


inhibition of root elongation




monodehydroascorbate reductase


programmed cell death


plasma membrane




reactive oxygen species


relative root elongation


superoxide dismutase


thiobarbituric acid


trichloroacetic acid


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This research was financially supported by the National Basic Research Program of China (the 973 Program, No. 2007CB109305), National Natural Science Foundation of China (grant Nos. 30771292, 30900920, 30270784), the Foundation for the University Ph.D.-Granting Discipline of the Ministry of Education (grant No. 20060335014) and IPNI. Great thanks are given to Prof. Caixian Tang of La Trobe University, Australia, for his critical review of the manuscript.

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Correspondence to X. Y. Lin.

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Xu, F.J., Li, G., Jin, C.W. et al. Aluminum-induced changes in reactive oxygen species accumulation, lipid peroxidation and antioxidant capacity in wheat root tips. Biol Plant 56, 89–96 (2012).

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