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Analysis of tolerance to copper and zinc in Aechmea blanchetiana grown in vitro

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Biologia Plantarum

Abstract

The aim of this study was to evaluate the growth and development of Aechmea blanchetiana Baker L.B. Sm. in vitro on medium with 0.0, 0.145, 1.45 and 14.5 μM Cu and 0.0, 2.75, 27.5 and 275 μM Zn. Significant accumulation of Cu and Zn occurred at 14.5 μM Cu and 27.5 and 275 μM Zn, respectively, and there were no significant changes in contents of the other macro- and micronutrients. Superoxide dismutase (SOD) activity significantly changed in the presence of both metals. Spermine content increased as Zn concentration increased and decreased with increasing concentrations of Cu. There was an accumulation of H2O2 in the leaf tissue of plants grown in 1.45 and 14.5 μM Cu and 27.5 and 275 μM Zn. A. blanchetiana was found tolerant to the Cu and Zn in concentrations used in this study and displays the capacity to accumulate these metals.

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Abbreviations

AA:

ascorbic acid

APX:

ascorbate peroxidase

CAT:

catalase

DAB:

3,3′-diaminobenzidine

MS:

Murashige and Skoog medium

Put:

putrescine

ROS:

reactive oxygen species

SOD:

superoxide dismutase

Spd:

spermidine

Spm:

spermine

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Acknowledgements

This study was supported by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq/Proc. 305776/2009-6) and Fundação de Auxílio à Pesquisa de São Paulo (FAPESP).

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Authors and Affiliations

  1. Instituto de Botânica, P.O. Box 3005, 01061-970, São Paulo, SP, Brazil

    S. Kanashiro, E. S. Alves, M. Domingos & A. R. Tavares

  2. Programa de Pós-graduação em Biodiversidade Vegetal e Meio Ambiente, P.O. Box 3005, 01061-970, São Paulo, SP, Brazil

    P. Giampaoli & F. Tresmondi

  3. Universidade Estadual Paulista, P.O. Box 510, 18618-970, Botucatu, SP, Brazil

    G. P. P. Lima

Authors
  1. P. Giampaoli
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  2. F. Tresmondi
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  3. G. P. P. Lima
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  4. S. Kanashiro
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  5. E. S. Alves
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  6. M. Domingos
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  7. A. R. Tavares
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Correspondence to A. R. Tavares.

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Giampaoli, P., Tresmondi, F., Lima, G.P.P. et al. Analysis of tolerance to copper and zinc in Aechmea blanchetiana grown in vitro . Biol Plant 56, 83–88 (2012). https://doi.org/10.1007/s10535-012-0020-7

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  • DOI: https://doi.org/10.1007/s10535-012-0020-7

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