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Isolation and functional characterization of MxCS1: a gene encoding a citrate synthase in Malus xiaojinensis

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Biologia Plantarum

Abstract

Iron is one of the essential micronutrients required by all living organisms. In this study, we isolated a gene encoding putative citrate synthase (CS) from Malus xiaojinensis, designated as MxCS1. The MxCS1 gene encodes a protein of 473 amino acid residues with a predicted molecular mass of 52.5 kDa and a theoretical isoelectric point of 8.67. The expression of MxCS1 was enriched in the leaf rather than in phloem and root, however, its expression was hardly detected in the xylem. The gene expression was strongly induced by Fe stress treatment in the M. xiaojinensis seedlings. Over-expression of MxCS1 improved Fe deficiency tolerance in transgenic Arabidopsis. We argued that MxCS1 is a new member of the CS genes, and it may function as a regulator in response to iron stress in plants.

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Abbreviations

BA:

6-benzylaminopurine

CaMV:

the cauliflower mosaic virus

CS:

citrate synthase

IBA:

indolebutyric acid

MS:

Murashige and Skoog

ORF:

open reading frame

RACE:

rapid amplification of cDNA ends

RT-PCR:

reverse transcriptasepolymerase chain reaction

WT:

wild type

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Acknowledgements

This project was supported by the genetically modified organisms breeding major projects (2009ZX08009-122B); New stars on Science and Technology of Beijing (2008B74); The National Natural Science Foundational of China (30800741) and Chinese universities scientific funds (2009-2-06).

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Authors and Affiliations

  1. Institute for Horticultural Plants, China Agricultural University and Key Laboratory of Beijing Municipality of Stress Physiology and Molecular Biology for Fruit Tree, Beijing, 100193, P.R. China

    D. -G. Han, Y. Wang, L. Zhang, L. Ma, X. -Z. Zhang, X. -F. Xu & Z. -H. Han

  2. College of Horticulture, Northeast Agricultural University, Haerbin, 150030, P.R. China

    D. -G. Han

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  1. D. -G. Han
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  2. Y. Wang
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  3. L. Zhang
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  4. L. Ma
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  5. X. -Z. Zhang
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  6. X. -F. Xu
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  7. Z. -H. Han
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Correspondence to Z. -H. Han.

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Han, D.G., Wang, Y., Zhang, L. et al. Isolation and functional characterization of MxCS1: a gene encoding a citrate synthase in Malus xiaojinensis . Biol Plant 56, 50–56 (2012). https://doi.org/10.1007/s10535-012-0015-4

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  • DOI: https://doi.org/10.1007/s10535-012-0015-4

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