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In vitro regeneration of Anethum graveolens, antioxidative enzymes during organogenesis and RAPD analysis for clonal fidelity

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Biologia Plantarum


An efficient in vitro regeneration protocol was developed for medicinally important aromatic plant Anethum graveolens. Nodal segments were cultured onto Murashige and Skoog (MS) basal medium supplemented with different auxins and cytokinins singly as well as in combinations. The optimum callus induction (93.33 %) was obtained on medium fortified with 2.2 μM N6-benzyladenine (BA) and 0.21 μM α-naphthaleneacetic acid. The best shoot regeneration (85.7 %) with 12.86 shoots per explant was achieved in two weeks when callus was subcultured on MS medium amended with 2.2 μM BA and 1.85 μM kinetin. The average length of regenerated shoots varied from 3.15 to 4.8 cm. The rooting of regenerated shoots was nearly 100 % on ¼ MS augmented with 4.9 μM indolebutyric acid with a maximum root length of 5.1 cm. Plantlets were successfully acclimatized with 60 % survival rate. During organogenesis, catalase and ascorbate peroxidase activity increased while superoxid dismutase activity decreased. Clonal fidelity of in vitro raised plants has been checked by random amplified polymorphic DNA using 10 selected decamer primers. It has been found that regenerated plants are true to type plants.

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scorbate peroxidase






cetyltrimethylammonium bromide


2,4-dichlorophenoxyacetic acid


ethidium bromide


indoleacetic acid


indolebutyric acid




Murashige and Skoog


α-naphthaleneacetic acid


polymerase chain reaction


random amplified polymorphic DNA


superoxidase dismutase.


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The financial support provided by Department of Science and Technology, New Delhi for the Project “Banasthali Centre for Education and Research in Basic Science” under their CURIE Programme is gratefully acknowledged. G.S. Shekhawat also acknowledges financial support received from the Department of Science and Technology, Rajasthan, Jaipur.

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Jana, S., Shekhawat, G.S. In vitro regeneration of Anethum graveolens, antioxidative enzymes during organogenesis and RAPD analysis for clonal fidelity. Biol Plant 56, 9–14 (2012).

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