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Molecular characterization of a gene induced during wheat hypersensitive reaction to stripe rust

  • Published:
Biologia Plantarum

Abstract

A novel gene induced during hypersensitive reaction (HIR) in wheat was identified using in silico cloning and designated as TaHIR2. The TaHIR2 gene was deduced to encode a 284-amino acid protein, whose molecular mass and isoelectric point (pI) were 31.05 kD and 5.18, respectively. Amino acid sequence analysis demonstrated the presence of stomatins, prohibitin, flotillins, HflK/C (SPFH) domain and prohibitin homologue for the TaHIR2 protein. Phylogenetic analysis of 13 HIR genes from different monocots indicated that TaHIR2 was highly homologous to HvHIR2. Transient expression analysis using particle-mediated bombardment showed that the TaHIR2 fusion protein was located in the onion epidermal cells. Quantitative RT-PCR analyses revealed that TaHIR2 transcripts were significantly accumulated in adult wheat leaves with maximum induction at 18 h post inoculation with the stripe rust, whereas slightly up-regulation could also be observed in the compatible reaction at the seedling stage. These results suggest that TaHIR2 may play an active role in wheat defense against stripe rust.

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Abbreviations

CT :

threshold value

GS:

growth stage

HIR:

hypersensitive induced reaction

hpi:

hour post inoculation

HR:

hypersensitive response

ORF:

open reading frame

qRT-PCR:

quantitative reverse transcription polymerase chain reaction

SAR:

systemic acquired response

RT-PCR:

reverse transcription PCR

SPFH:

stomatins, prohibitin, flotillins, HflK/C

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Acknowledgements

This study was supported by grants from the National Natural Science Foundation of China (30930064 and 30800712), the National Basic Research Program of China (2006CB708208), the National High Technology Research and Development Program of China (863 Program, 2006AA10A104), the earmarked fund for Modern Agro-industry Technology Research System, the Program for Changjiang Scholars and Innovative Research Team in University, Ministry of Education of China (200558), and the 111 Project from the Ministry of Education of China (B07049). The first two authors contributed equally to this paper.

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Author notes

  1. G. Zhang

    Present address: College of Pharmacy, Shaanxi University of Chinese Medicine, Xianyang, Shaanxi, 712046, China

  2. Y. -M. Li

    Present address: Division of Science & Technology, Shaanxi University of Chinese Medicine, Xianyang, Shaanxi, 712046, China

  3. J. -M. Wang

    Present address: Institute of Plant Protection, Henan Provincial Academy of Agricultural Sciences, Zhengzhou, Henan, 450002, China

  4. B. Liu

    Present address: Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

Authors and Affiliations

  1. State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, Shaanxi, 712100, P. R. China

    G. Zhang, Y. -M. Li, Y. -F. Sun, J. -M. Wang, B. Liu, J. Zhao, J. Guo, L. -L. Huang & Z. -S. Kang

  2. USDA-ARS and Department of Plant Pathology, Washington State University, Pullman, WA, 99164-6430, USA

    X. -M. Chen

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  1. G. Zhang
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Correspondence to Z. -S. Kang.

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Zhang, G., Li, Y.M., Sun, Y.F. et al. Molecular characterization of a gene induced during wheat hypersensitive reaction to stripe rust. Biol Plant 55, 696–702 (2011). https://doi.org/10.1007/s10535-011-0170-z

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  • DOI: https://doi.org/10.1007/s10535-011-0170-z

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